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TFORF0297

Plasmid

#141565

Purpose

Lentiviral vector for overexpressing the CREM transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CREM (CREM Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-Blast

Plasmid

#171952

Purpose

All-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, truncated SRSF3

Depositor

Sujatha Jagannathan

Article

Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

Insert

serine and arginine rich splicing factor 3 (SRSF3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized, truncated at amino acid 114 with…

Promoter

TRE

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pJA14

Plasmid

#59929

Purpose

gRNA for cleavage at rde-1(H974) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(H974) gRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mouse NT

Plasmid

#242421

Purpose

Expression of a non-targeting shRNA in mouse cells

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

Non-targeting shRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1a FLAG-CHMP2B

Plasmid

#232012

Purpose

Expression of FLAG-tagged CHMP2B

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniSOG-C-Far

Plasmid

#193914

Purpose

Control plasmid that expresses miniSOG targeted to the cell membrane in mammalian cells

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Farnesylation signal sequence and Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniSOG

Plasmid

#193913

Purpose

Control plasmid that expresses non-targeted miniSOG in mammalian cells

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET11-PC-H6

Plasmid

#183777

Purpose

Express PC-H6 in E. coli. Purified PC-H6 was used for for generating antibodies in rabbits and for GST-CBP pull-down assays.

Depositor

Peter Harte

Article

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb (Pc Fly)

Tags

6 His tag at the C-terminus

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMIG II-BTLA-mGFP

Plasmid

#180798

Purpose

Expression of mouse BTLA fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLA (Btla Mouse)

Use

Retroviral

Tags

mEGFP

Promoter

MSCV

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A

Plasmid

#163988

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

M1457A

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B CARD8 UPA-CARD-mCherry R464E

Plasmid

#164013

Purpose

Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

mCherry

Expression

Mammalian

Mutation

R464E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

Plasmid

#163991

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 CARD (NLRP1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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Zebrafish-gRNA-0009

Plasmid

#42249

Purpose

gRNA targeted to zebrafish gene tph1a

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-tph1a (tph1a Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only
pET28a_SUMO_AcCas12n

Plasmid

#203808

Purpose

pET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

SUMO_AcCas12n

Use

CRISPR

Promoter

T7

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet-SARS-CoV-2-SL1 alone

Plasmid

#191485

Purpose

Lentiviral expression of SARS-CoV-2 5_ UTR SL1 alone

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

SARS-CoV-2 5' UTR SL1 alone (NEWENTRY Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

Plasmid

#176839

Purpose

To recruit SAM complex to human CYP3A4 proximal enhancer.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer sgRNA for activation

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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yap1_R (OZ590)

Plasmid

#35230

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting yap1 (yap1 Zebrafish)

Use

Zebrafish targeting

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
yap1_L (OZ589)

Plasmid

#35229

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting yap1 (yap1 Zebrafish)

Use

Zebrafish targeting

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
NFATc1_L (OZ543)

Plasmid

#28068

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting NFATc1 (nfatc1 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
NFATc1_R (OZ544)

Plasmid

#28069

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting NFATc1 (nfatc1 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only