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p3XFlag-Mettl4

Plasmid

#86665

Purpose

Expresses murine Mettl4 containing N-terminal 3XFlag tag

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

Mettl4 (Mettl4 Mouse)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-ciRS7 Exon

Plasmid

#69900

Purpose

Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ciRS7 (stw, CDR1-AS Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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HCA3-Tango

Plasmid

#66397

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

HCA3 (HCAR3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pMTTH+H1.4ΔCTD

Plasmid

#157797

Purpose

Expression of human linker histone H1.4 without c-terminal tail

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H1.4ΔCTD (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-Climp63(1–192)

Plasmid

#186955

Purpose

Expresses mCherry-Climp63 with partial luminal domain

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Ckap4 (Ckap4 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only remain partial luminal domain

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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V5-bArr1

Plasmid

#201491

Purpose

Expression of the beta-arrestin1 containing a N-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

V5-bArr1 (ARRB1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-MTOR HEAT

Plasmid

#120709

Purpose

Expresses Flag-HA-6xHIS-Mtor HEAT Domain fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MTOR HEAT 1-9 (MTOR Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Amino acids 1-409

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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p5072 pcDNA4c hBrd4 (1224-1362)

Plasmid

#14451

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1224-1362) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1224-1362

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
p5082 pcDNA4c hBrd4 (1047-1133)

Plasmid

#14458

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1047-1133) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1047-1133

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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p3xFlag-Alkbh4

Plasmid

#105240

Purpose

Expresses murine Alkbh4 containing an N-ternimal 3xFlag tag

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

Alkbh4 (Alkbh4 Mouse)

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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p5080 pcDNA4c hBrd4 (1134-1362)

Plasmid

#14456

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1134-1362) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1134-1362

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
TPI-PTC1

Plasmid

#130699

Purpose

Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid)

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

TPI-PTC1 (TPI1 Human)

Expression

Mammalian

Mutation

PTC1

Promoter

Pcmv

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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HP21-TPI-WT-no_introns

Plasmid

#131044

Purpose

Expresses TPI-Wildtype NMD reporter without introns and with a 21 nt hairpin in 5'UTR

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

TPI-Wildtype-no introns (TPI1 Human)

Expression

Mammalian

Promoter

Pcmv

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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p5073 pcDNA4c hBrd4 (1047-1223)

Plasmid

#14452

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1047-1223) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1047-1223

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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p5081 pcDNA4c hBrd4 (1134-1223)

Plasmid

#14457

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1134-1223) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1134-1223

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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ps226

Plasmid

#133557

Purpose

RNAi construct for knock-down of CDKN1A (Cip1, p21)

Depositor

David Livingston

Article

Chan et al Genes Dev. 2005 Jan 15;19(2):196-201. do

Insert

CDKN1A (CDKN1A Human)

Use

Retroviral

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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mKO-Mito-7

Plasmid

#57848

Purpose

Localization: Mitochondria, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Mito (COX8A Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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GST-GAR

Plasmid

#34697

Depositor

Steven Clarke

Article

Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-8

Insert

Fibrillarin (FBL Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-145 of human fibrillarin with K2E and …

Promoter

tac

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only
mTFP1-H3-23

Plasmid

#55489

Purpose

Localization: Nucleus/Histones, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

H3 (H3C2 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCI-FLAG-mRRP6

Plasmid

#60045

Purpose

Mammalian expression of FLAG-tagged mouse RRP6

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

RRP6 (Exosc10 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only