We narrowed to 30,799 results for: Ide

1. pUB-nHyPer2 (NLS:HyPer2:NLS)

   Plasmid

   #84730

   Purpose

   Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   HyPer2 targeted to nucleus
   

   Tags

   NLS (nuclear localisation signal and NLS (nuclear…

   Expression

   Plant

   Promoter

   ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA_DNMT3a-T2

   Plasmid

   #41822

   Purpose

   Expresses a guide RNA (gRNA) to target DNMT3a (T2 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_DNMT3a-T2 (DNMT3A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

3. gRNA_DNMT3b

   Plasmid

   #41823

   Purpose

   Expresses a guide RNA (gRNA) to target DNMT3b for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_DNMT3b (DNMT3B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pEJS2603_AAV-Nme2-ABE8e-i1-U6-Rosa26

   Plasmid

   #201685

   Purpose

   All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 driven sgRNA targeting the mouse Rosa26 gene

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-i1
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDECKO_mCherry_CD19.4

   Plasmid

   #140101

   Purpose

   CRISPR-Cas9 Knock down

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. psgRNA (GB0645)

   Plasmid

   #68210

   Purpose

   Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   SgRNA
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. A3B-Cas9n-UGI-NLS

   Plasmid

   #198889

   Purpose

   Express full-length A3B BE3-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
   

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-Lmo2

   Plasmid

   #60666

   Purpose

   PiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoter

   Depositor

   James Thomson

   Article

   Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

   Insert

   Lmo2 (Lmo2 Mouse)
   

   Use

   Piggybac

   Expression

   Mammalian

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDECKO-mCherry MALAT1_Enhancer.1

   Plasmid

   #78536

   Purpose

   Experimental plasmid

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (for expressing sgRNA) and U6 promoter

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCold I-Hero7

    Plasmid

    #187926

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero7

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero7 (SERF2 Human)
    

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p7612 MSCV-P C-FlagHA 16E7 E10K

    Plasmid

    #163303

    Purpose

    Expresses HPV16 E7 E10K

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    HPV16 E7 E10K
    

    Use

    Retroviral

    Tags

    FlagHA

    Mutation

    E10K

    Promoter

    MSCV LTR

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDECKO-mCherry MALAT1_Exon.1

    Plasmid

    #78540

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-KD-mAbl

    Plasmid

    #69028

    Purpose

    Retorviral vector encoding Kinase Defective mouse c-Abl

    Depositor

    Jean Wang

    Article

    Sridevi et al PLoS One. 2013 Oct 11;8(10):e77495. doi:

    Insert

    mouse c-Abl Type VI - kinase defective
    

    Use

    Retroviral

    Tags

    FLAG tag inserted at Internal SalI site (2934 bp)

    Expression

    Mammalian

    Mutation

    The KD (kinase defective) mutation is at Lysine 2…

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

14. mutpRL-TK 3xUTR

    Plasmid

    #40758

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    3 copies of the CXCR4 small RNA target site
    

    Use

    Luciferase

    Tags

    luciferase

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available Since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pGL2-basic mt E2F-luciferase

    Plasmid

    #133708

    Purpose

    Expression of E2F1-mt-luc.

    Depositor

    James A. DeCaprio

    Article

    Zalvide et al Mol Cell Biol 1998 Mar;18(3):1408-15.

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Trxn factor bind site

    Expression

    Mammalian

    Mutation

    the three binding site copies are mutated form

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPTXcGMPRELUC

    Plasmid

    #68503

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
    

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

    Promoter

    OPTX promoter with cGMPRE

    Available Since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-Xl-VSP1 R152Q GFP

    Plasmid

    #51884

    Purpose

    expression of Xl-VSP1 mutant fused to GFP in Xenopus oocytes or mammalian cells

    Depositor

    Laurinda Jaffe

    Article

    Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

    Insert

    VSP1 R152Q (tpte2 Frog)
    

    Use

    Xenopus oocyte expression

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R152Q: near the extracellular side of the fourth …

    Promoter

    CMV

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDECKO-mCherry TFRC_B

    Plasmid

    #78544

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPTXGARE

    Plasmid

    #68542

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter with GARE (AT1G33440 Mustard Weed)
    

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    5x GARE inserted 190 bp 5' pf ATG (GARE = ta…

    Promoter

    OPTX promoter with GARE

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDECKO-mCherry MALAT1_Exon.2

    Plasmid

    #78541

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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