We narrowed to 26,892 results for: EMB

1. mbYFPQS

   Plasmid

   #80742

   Purpose

   membrane-targeted biosensor for monitoring changes in intracellular chloride

   Depositor

   Susan Amara, Susan Ingram

   Article

   Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371

   Insert

   YFPQS
   

   Tags

   palmitoylation signal sequence of neuromodulin

   Expression

   Mammalian

   Mutation

   H148Q (CAC to CAG) and V163S (GTG to AGC).

   Promoter

   CMV

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pMM2-hPMCA1b

   Plasmid

   #47758

   Purpose

   mammalian expression of human PMCA1b

   Depositor

   John Penniston, Emanuel Strehler

   Article

   Adamo et al Biochem J. 1992 Aug 1;285 ( Pt 3):791-7.

   Insert

   PMCA1 (ATP2B1 Human)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV116

   Plasmid

   #63183

   Purpose

   pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-hKCNE1

   Plasmid

   #53050

   Purpose

   Expresses beta subunit for IKs channel in mammalian cells

   Depositor

   Michael Sanguinetti

   Article

   Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

   Insert

   Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-BiNLS-fYap

   Plasmid

   #176623

   Purpose

   expresses optofYap in zebrafish

   Depositor

   Timothy Saunders

   Article

   Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

   Insert

   Yes associated protein (yap1 Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   mCherry

   Promoter

   SP6

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. MAC_C_TYRO3

   Plasmid

   #187793

   Purpose

   MAC-tagged gene expression of human TYRO3

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   TYRO3 (TYRO3 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pN1-Lck-GCaMP3

   Plasmid

   #26974

   Depositor

   Baljit Khakh

   Article

   Shigetomi et al Neuron Glia Biol. 2010 Dec 16:1-9. [Epub

   Insert

   Lck-GCaMP3
   

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   Plasmid contains N-terminal 26 amino acid membran…

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAV125

   Plasmid

   #63191

   Purpose

   pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. NET29A.Hs pEGFP-N2 (1181)

   Plasmid

   #62040

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

   Insert

   NET29A (TMEM120A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Splice variant

   Promoter

   CMV

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDS2142

    Plasmid

    #184273

    Purpose

    Tet-off genome insertion system

    Depositor

    Dominique Sanglard

    Article

    Lemberg et al PLoS Pathog. 2022 Apr 11;18(4):e1010012.

    Insert

    CatTA
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMV_AA089

    Plasmid

    #216105

    Purpose

    gRNA expression for Cas12a (guide only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    gRNA expression
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBTK107

    Plasmid

    #110575

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    CP25 Promoter with RBS
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. MAC_C_EphA7

    Plasmid

    #187765

    Purpose

    MAC-tagged gene expression of human EphA7

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    EphA7 (EPHA7 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBTK519

    Plasmid

    #110603

    Purpose

    BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. MAC_C_MUSK

    Plasmid

    #187781

    Purpose

    MAC-tagged gene expression of human MUSK

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    MUSK (MUSK Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. myc-hIFITM3-delta59-68

    Plasmid

    #104361

    Purpose

    Expresses human IFITM3-delta59-68 with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

    Insert

    IFITM3 (IFITM3 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    deleted amino acids 59 through 68

    Promoter

    CMV

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOPS0254

    Plasmid

    #115506

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celB

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Rlv3841pNifH pOGG082, celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCIP-mB2M

    Plasmid

    #154091

    Purpose

    Self-Cutting and Integrating CRISPR backbone targeting murine B2M

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    sgRNA and BAIT targeting murine B2M locus
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-IRAlus-Apobec3G

    Plasmid

    #92349

    Purpose

    Plasmid for expression of GFP gene with a Apobec3G gene intron derived IR Alu element containing 3'UTR.

    Depositor

    Gordon Carmichael, Ling-Ling Chen

    Article

    Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

    Insert

    Apobec3G gene intron derived IR Alu element containing 3'UTR (APOBEC3G Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-Alus-Apobec3G

    Plasmid

    #92348

    Purpose

    Plasmid for expression of GFP gene with a Apobec3G gene intron derived Alu element containing 3'UTR.

    Depositor

    Gordon Carmichael, Ling-Ling Chen

    Article

    Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

    Insert

    Apobec3G gene intron derived Alu element containing 3'UTR (APOBEC3G Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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