We narrowed to 25,030 results for: Nes

1. pRK5-HA-Ubiquitin-KO

   Plasmid

   #17603

   Purpose

   Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   KO: all lysines mutated to arginines

   Promoter

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. hCMV-IE1:FLuc

   Plasmid

   #118063

   Purpose

   Transcriptional unit for constitutive expression of FLuc in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:FLuc:bGHF
   

   Use

   Luciferase and Synthetic Biology; Assembly of gb2…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-dCas9

   Plasmid

   #47106

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Flag and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-sbGLuc-B7-EYFP

   Plasmid

   #114110

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Gomez-Ramirez et al J Neurosci Res. 2020 Mar;98(3):471-480.

   Inserts

   Gaussia luciferase, slow burn
   B7 hinge region
   Enhanced Yellow Fluorescent Protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV GFAP CaBLAM

   Plasmid

   #244228

   Purpose

   Bioluminescent reporter for calcium signaling in astrocytes

   Depositor

   Ute Hochgeschwender

   Article

   Lambert et al bioRxiv. 2023 Jun 26:2023.06.25.546478.

   Inserts

   mNeonGreen
   CaBLAM
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6xHis-SNAP-PI(3)P Probe

   Plasmid

   #211508

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   FYVE-Hrs x 2 (PIKFYVE Human)
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pV1524

   Plasmid

   #111431

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pV1393

   Plasmid

   #111430

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDule2-Acd82

   Plasmid

   #174100

   Purpose

   Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

   Depositor

   Ryan Mehl

   Article

   Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

   Insert

   Acridone 82 tRNA synthetase and tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N311S, C313G, V366A, W382T

   Promoter

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-FLEX-SaCas9-U6-sgSlc17a7

    Plasmid

    #124846

    Purpose

    Mutagenesis of Slc17a7

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a7 (Slc17a7 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. YE1-BE4max-NG

    Plasmid

    #138159

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A + …

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. AALN-BE4max

    Plasmid

    #138161

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(AALN)-nSpCas9-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 R33A + K34A + H122L + D124N; with…

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPathBrick

    Plasmid

    #65006

    Purpose

    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive native promoters

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC1-SypHer3s-dmito

    Plasmid

    #108119

    Purpose

    Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s-dmito
    

    Use

    Tags

    ratiometric YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC-GFP-AT

    Plasmid

    #133306

    Purpose

    "Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

    Depositor

    Chris Barnes

    Article

    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

    Insert

    axe/txe toxin-antitoxin cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T-1 3xFlag-STAT1

    Plasmid

    #82722

    Purpose

    Expresses human STAT1

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Tags

    3xFLAG and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

17. AAEL010097-Cas9

    Plasmid

    #100707

    Purpose

    The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL010097-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL010097 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEasyG2_mic

    Plasmid

    #184916

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. 7xSMAD_RE::FLuc

    Plasmid

    #124531

    Purpose

    SMAD2 FLuc luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    SMAD FLuc reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMax_mOrange_G299C

    Plasmid

    #177829

    Purpose

    Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mOrange_G299C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G299C

    Promoter

    CMV

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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