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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,765 results for: Pol

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Showing: 4241 - 4260 of 7765 results

  1. pCAG-Halo-Tag-128-425-Akap9

    Plasmid
    #196873
    Purpose
    Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to Halo-Tag. Used to displace endogenous Akap9 from Golgi
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Halo-Tag-Akap9 (128-425) (Akap9 Rat)
    Expression
    Mammalian
    Promoter
    Quimeric CAG
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196873-plasmid-map-sequence-id-390445

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  2. pBactin-Nedd1-mOrange2-hCM1

    Plasmid
    #196863
    Purpose
    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nedd1-mOrange2-hCM1 (Nedd1 Human, Rat)
    Expression
    Mammalian
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196863-plasmid-map-sequence-id-391832

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  3. AV44_pCAG.Cas9-D10A.gRNA.S1

    Plasmid
    #199258
    Purpose
    Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SpCas9-D10A nickase
    AAVS1-targeting gRNA
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CAG promoter and RNA polymerase III promoter for …
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199258-plasmid-map-sequence-id-391654

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  4. pBetaActin-FKBP-mScarlet-Dync1h1motor

    Plasmid
    #191330
    Purpose
    Expresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Publication
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
    Expression
    Mammalian
    Mutation
    DYNC1H1 motor domain aa 1453-4644
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    191330-plasmid-map-sequence-id-381732

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  5. pBa.Halo-Kif13B tail 442-1843

    Plasmid
    #162721
    Purpose
    Mammalian expression of WT mouse Kif13b with deletion of the 441 N-terminal residues
    Depositor
    Marvin Bentley
    Publication
    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Kif 13B (Kif13b Mouse)
    Expression
    Mammalian
    Mutation
    WT with deletion of the 441 N-terminal residues.
    Promoter
    Chicken Beta actin
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    162721-plasmid-map-sequence-id-319476

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  8. pGL4.23-CD69 CaRE3 (minus strand)

    Plasmid
    #91845
    Purpose
    Luciferase reporter for CD69 enhancer (IGI-P0622)
    Depositor
    Jacob Corn
    Publication
    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD69 CaRE3 (minus strand) (CD69 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Missing 1 base from 13 base polyT tract beginning…
    Available since
    May 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    91845-plasmid-map-sequence-id-179912

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  12. pGL4_10xUAS-CMVmin-BC0396-luc2

    Plasmid
    #227115
    Purpose
    Barcoded assay, UAS sensor for split TEV assays; barcode BC0396
    Depositor
    Michael Wehr
    Publication
    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    10x clustered UAS element linked to CMV minimal promoter driving barcode 0396 and a luciferase reporter gene
    Expression
    Mammalian
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227115-plasmid-map-sequence-id-455201

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  14. pKE13-ISceI-fabp10a:CreERT2

    Plasmid
    #198242
    Purpose
    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter driving tamoxifen-inducible Cre recombinase
    Depositor
    Didier Stainier
    Publication
    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.
    Inserts
    Zebrafish fabp10a promoter:Cre recombinase (Cre) fused to a mutant estrogen ligand-binding domain (ERT2), polyA (fabp10a Zebrafish)
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
    Use
    Zebrafish transgenesis
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    198242-plasmid-map-sequence-id-393628

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  15. pB80-hsKIF5B(1-560)Rigor-L-2xHA

    Plasmid
    #193718
    Purpose
    Expression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules
    Depositor
    Lukas Kapitein
    Publication
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hsKIF5B(1-560)Rigor-L-2xHA (KIF5B Synthetic, Human)
    Tags
    2xHA
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193718-plasmid-map-sequence-id-383730

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  16. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid
    #190598
    Purpose
    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter
    Depositor
    Claudia Rueckert
    Publication
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    mCherry - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190598-plasmid-map-sequence-id-379953

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  20. pB80-hsKIF5B(1-560)Rigor-L-mVenus

    Plasmid
    #193720
    Purpose
    Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules
    Depositor
    Lukas Kapitein
    Publication
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hsKIF5B(1-560)Rigor-L-mVenus (KIF5B Synthetic, Human)
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193720-plasmid-map-sequence-id-383731

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Showing: 4241 - 4260 of 7765 results