We narrowed to 10,324 results for: otos

1. pAP1638

   Plasmid

   #137089

   Purpose

   Plasmid for PCR-generated donor DNA for rapid tagging of proteins with 4*(GFP11)

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

   Insert

   4*(GFP11)
   

   Use

   Pcr template for donor dna

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDest_pK7WG2_pRPS5A_CTP OleGFP

   Plasmid

   #171723

   Purpose

   ptpTALECD vector assembly, Destination vector;

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

   Insert

   RPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. E2_pENTR_R4R3_T CTP

   Plasmid

   #171736

   Purpose

   ptpTALECD vector assembly, Entry clone 2 with attR4 and R3

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

   Insert

   A terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal
   

   Use

   Multisite gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. mOrange2-Endo-14

   Plasmid

   #57955

   Purpose

   Localization: Endosomes, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Endo (RHOB Human)
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pAP1836

   Plasmid

   #137087

   Purpose

   Plasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherry

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

   Insert

   mCherry
   

   Use

   Pcr template for donor dna

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS315e_pGal-dCas9-SH3

   Plasmid

   #79613

   Purpose

   Expresses dCas9-SH3 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   SH3 domain

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS426-TUB1-internal6xHis

   Plasmid

   #60390

   Purpose

   Expression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB1 (TUB1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   internal 6xHis (see supplement figure 4)

   Promoter

   GAL

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-hSyn-ChrimsonR-LSSmOrange

   Plasmid

   #149691

   Purpose

   For packaging and expression of ChrimsonR-LSSmOrange in neurons via AAV

   Depositor

   Robert Malenka

   Article

   Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

   Insert

   ChrimsonR
   

   Use

   AAV

   Tags

   LSSmOrange

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGFPmAID_htz-1

   Plasmid

   #174214

   Purpose

   Donor plasmid for tagging htz-1 with GFP::mAID

   Depositor

   Masato Kanemaki

   Article

   Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

   Insert

   GFP::mAID::htz-1 tagging donor
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFLIPglu-3.2mDelta13

    Plasmid

    #12992

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A S112A Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    F16A, S112A in binding protein

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

11. Khc73-GFP

    Plasmid

    #31746

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Khc73 (Khc-73 Fly)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Metallothionein

    Available since

    Feb. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pFLIPglu-170nDelta13

    Plasmid

    #12989

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16F Delta13
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    WT binding protein (no F16A)

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pJAZ10::LUC

    Plasmid

    #141172

    Purpose

    Luminescent reporter for jasmonic acid signaling

    Depositor

    Patrick Schäfer, Vardis Ntoukakis

    Article

    Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

    Insert

    AtJAZ10 promoter
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtJAZ10, AT5G13220

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJH3577

    Plasmid

    #179612

    Purpose

    Pceh-12::Chrimson::Cherry unc-54 3' UTR C.elegans VB-MNs expression of Chrimson::Cherry

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    Chrimson
    

    Use

    Tags

    Cherry

    Expression

    Worm

    Mutation

    Promoter

    Pceh-12

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-TAF4(827-909)

    Plasmid

    #105620

    Purpose

    bacterially express TAF4 HFD

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF4-HFD(827-909) (Taf4 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T1-TAF12(50-130)

    Plasmid

    #105618

    Purpose

    bacterially express GST tagged TAF12 HFD

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF12-HFD amino acid 50-130 (Taf12 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mOrange2-Vinculin-23

    Plasmid

    #57978

    Purpose

    Localization: Focal Adhesions, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    V234L in vinculin

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pFIN-EF1a-CHER-WPRE

    Plasmid

    #44353

    Depositor

    Susan Semple-Rowland

    Article

    Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

    Insert

    EF1a-CHER
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    human elongation factor -1a (pos 2526-3969)

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMB38-HF380

    Plasmid

    #37332

    Depositor

    Takahide Kon

    Article

    Kon et al Nature. 2012 Mar 7;484(7394):345-50. doi

    Insert

    Cytoplasmic Dynein Heavy Chain (dhcA Dictyostelium discoideum)
    

    Use

    Dictyostelium expression

    Tags

    His6 and FLAG tag

    Expression

    Mutation

    deleted amino acids 1-1387

    Promoter

    TRE-Pmin

    Available since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

20. Tol2 8xSTAR-mScarletI. PGK-puro

    Plasmid

    #136266

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mScarletI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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