We narrowed to 7,284 results for: Ank

1. pAAV-GFAP-JEDI-2P-WPRE

   Plasmid

   #179469

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for astrocytes specific expression using the promoter GFAP

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Insert

   JEDI-2P
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   GFAP

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TMEM230-WT-DsRed(isoform 2)

   Plasmid

   #99563

   Purpose

   mammalian expression of TMEM230-dsRed (isoform 2)

   Depositor

   Han-Xiang Deng

   Article

   Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

   Insert

   TMEM230 (TMEM230 Human)
   

   Tags

   DsRed

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/Puro-CAG-JEDI-2P-Kv

   Plasmid

   #179462

   Purpose

   Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Insert

   JEDI-2P-Kv
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-Flag-CDK19-W105M-IRES-ZsGreen

   Plasmid

   #68859

   Purpose

   Expresses Human CDK19 W105M Mutant

   Depositor

   Matthew Shair

   Article

   Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

   Insert

   Cyclin-Dependent Kinase 19 (CDK19 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   W105M

   Promoter

   EF1α

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-DIO-PPO-Venus

   Plasmid

   #139504

   Purpose

   AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Has Service

   AAV5

   Insert

   parapinopsin
   

   Use

   AAV

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon)

   Plasmid

   #85800

   Purpose

   donorAB-UBC-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2Bneon

   Depositor

   Roland Friedel

   Article

   Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

   Insert

   PPP1R12C (PPP1R12C Human)
   

   Use

   Human targeting

   Mutation

   homology arms for knock-in into first intron

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-JEDI-2P-WPRE

   Plasmid

   #179466

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Insert

   JEDI-2P
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-FRT/TO-FLAG-SHLD3

   Plasmid

   #114123

   Purpose

   Expresses N-terminally tagged FLAG-SHLD3 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD3 (SHLD3 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV/TetO2

   Available Since

   Aug. 16, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA5-FRT/TO-FLAG-SHLD1

   Plasmid

   #114115

   Purpose

   Expresses N-terminally tagged FLAG-SHLD1 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD1 (SHLD1 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV/TetO2

   Available Since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. Mouse Alpha2 delta-2 pMT2

    Plasmid

    #58732

    Purpose

    expression of mouse Alpha2 delta-2 in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Barclay et al J Neurosci. 2001 Aug 15;21(16):6095-104.

    Insert

    alpha2 delta-2 (Cacna2d2 Mouse)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

    Plasmid

    #185706

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    Cas9
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. R620-M13-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.1.7

    Plasmid

    #170197

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    Δ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…

    Promoter

    CMV51

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUW TetO HA-AMPKa2 WT

    Plasmid

    #69826

    Purpose

    Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    minimal CMV promoter with tetracycline operator

    Available Since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT/TO-V5-SHLD3

    Plasmid

    #114124

    Purpose

    Expresses N-terminally tagged V5-SHLD3 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD3 (SHLD3 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV/TetO2

    Available Since

    Aug. 23, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. VARP GFP-pLXIN

    Plasmid

    #62950

    Purpose

    full length human VARP with C-terminal EGFP tag cloned into pLXIN

    Depositor

    Paul Luzio

    Article

    Hesketh et al Dev Cell. 2014 Jun 9;29(5):591-606. doi:

    Insert

    VARP (ANKRD27 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silent mutations of amino acids 422-428 to produc…

    Promoter

    LTR

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCJ209

    Plasmid

    #162682

    Purpose

    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Replacement of G251-T472 with W185-F191 from PETa…

    Promoter

    T7/lac

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW57-mCherry-2A-BRD4 Iso AdelCTD

    Plasmid

    #137722

    Purpose

    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)

    Depositor

    Scott Floyd

    Article

    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

    Insert

    BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Truncated at amino acid 1328

    Promoter

    Tight TRE promoter

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. TMEM230-WT-pEGFP(isoform 2)

    Plasmid

    #99562

    Purpose

    mammalian expression of TMEM230-GFP (isoform 2)

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. R620-M14-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.351

    Plasmid

    #170198

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

    Promoter

    CMV51

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST-N2H-F2-empty vector

    Plasmid

    #125552

    Purpose

    N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment2 alone without any attached prot…

    Expression

    Mammalian and Yeast

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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