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Bxb1-surrogate-reporter

Plasmid

#65624

Purpose

Fluorescent reporter to monitor the activty of Bxb1; If Bxb1 and attB donor plasmid containing GFP is present in the cell, recombination of the donor into the reporter leads to expression of GFP.

Depositor

Sebastian Bultmann

Publication

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attP

Use

Bxb1

Expression

Mammalian

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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FCK-QuasAr2-Citrine

Plasmid

#59172

Purpose

Fluorescent membrane voltage sensor

Depositor

Adam Cohen

Publication

Zou et al Nat Commun. 2014 Aug 13;5:4625. doi: 10.

Insert

QuasAr2-Citrine eFRET

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-G-Rac1

Plasmid

#180575

Purpose

ddGFP sensor of Rac1

Depositor

Won Do Heo

Publication

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Rac1

Expression

Mammalian

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-R-Rac1

Plasmid

#180576

Purpose

ddRFP sensor of Rac1

Depositor

Won Do Heo

Publication

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Rac1

Expression

Mammalian

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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Hygro-iDEST

Plasmid

#75339

Purpose

GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

Depositor

Danwei Huangfu

Publication

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

TRE-DEST

Expression

Mammalian

Promoter

TRE-tight

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-DUET-GST-eIF4G1-His6, eIF4E

Plasmid

#37232

Depositor

Jon Lorsch

Publication

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Inserts

TIF4631 (TIF4631 Budding Yeast)
CDC33 (CDC33 Budding Yeast)

Tags

GST and His6

Expression

Bacterial

Promoter

T7

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pUC57-CmYLCV

Plasmid

#205245

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Publication

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site

Use

CRISPR

Expression

Plant

Promoter

CmYLCV

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Geminin-mStrawberry reporter

Plasmid

#158690

Purpose

This plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Publication

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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FCK-QuasAr2-mOrange2 eFRET

Plasmid

#59173

Purpose

Fluorescent membrane voltage sensor

Depositor

Adam Cohen

Publication

Zou et al Nat Commun. 2014 Aug 13;5:4625. doi: 10.

Insert

QuasAr2-mOrange2 eFRET

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomEos2-Kan

Plasmid

#44944

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mEos2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomEos2

Expression

Yeast

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-OsU3m/LacZ

Plasmid

#66192

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Publication

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

OsU3m (SpeI site destroyed)

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pU6-sgGAL4-4

Plasmid

#46916

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)

Depositor

Stanley Qi, Jonathan Weissman

Publication

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgGAL4-4
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV and U6

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTYF-G4BS-2TPH-EGFP-IRES-GAL4p65

Plasmid

#32569

Depositor

Sergey Kasparov

Publication

Benzekhroufa et al Gene Ther. 2009 May;16(5):681-8. Epub 20

Inserts

EGFP
Gal4p65

Use

Lentiviral

Promoter

2TPH

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pAJE3-E7

Plasmid

#214359

Purpose

Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

Depositor

Ryan Mehl

Publication

Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

Inserts

2nd generation Tet2 "E7" aminoacyl tRNA synthetase
Lpp promoted M. jannaschii tRNA
Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
Orthogonal "D4II" ColE1 origin

Expression

Bacterial

Promoter

AmpR and lpp

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDule-Tet2.0

Plasmid

#85496

Purpose

Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Publication

Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044

Insert

Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Tags

None

Expression

Bacterial

Mutation

Y32G L65Q F108S Q109D D158S L162N

Promoter

lpp (constitutive)

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSA110

Plasmid

#59784

Purpose

drives mCherry in C. elegans intestinal cells

Depositor

Jeremy Nance

Publication

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

elt-2 promoter (elt-2 Nematode)
mCherry (worm)
unc-54 3' UTR

Expression

Bacterial

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pGEP163

Plasmid

#137881

Purpose

PiggyBac donor vector for expression of mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Publication

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

mRFP-T2A-BlasticidinR

Expression

Mammalian

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMA-SpCas9-g4

Plasmid

#80787

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array

Depositor

Yonglun Luo

Publication

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-MS2-PP7

Plasmid

#75392

Purpose

sgRNA1-MS2-PP7

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XMS2-PP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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TRUPATH Triple Ga11

Plasmid

#196059

Purpose

Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

Depositor

Justin English

Publication

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Inserts

GAlpha11-RLuc8 (GNA11 Human)
GGamma13-GFP2 (GNG13 Human)
GBeta3 (GNB3 Human)

Use

Luciferase

Tags

GFP2 and GSAG linker

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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