We narrowed to 15,891 results for: NOL

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pCfB6638

Plasmid

#106165

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G07

Plasmid

#67141

Purpose

Gateway destination vector for cell-free expression with N-term myc-tag mCherry tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His-MYC and mCherry

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pUCBB-ntH6-eGFP

Plasmid

#32557

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST-hPDI

Plasmid

#60555

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Article

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Tags

His6-hPDI

Expression

Bacterial

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pc-GAKIN-Ha

Plasmid

#188246

Purpose

Express GAKIN in mammalian cells with Ha tag

Depositor

Joel Pomerantz

Article

Lamason et al Mol Cell. 2010 Dec 10;40(5):798-809. doi

Insert

GAKIN (KIF13B Human)

Tags

Ha

Expression

Mammalian

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMB1-spect-PthrC-eGFP

Plasmid

#107408

Purpose

This plasmid contain promoter PthrC, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Article

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Expression

Bacterial

Promoter

PthrC

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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HIR Y1158F

Plasmid

#24051

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Expression

Mammalian

Mutation

Y1158F

Available Since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only
HIR Y1162F

Plasmid

#24052

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Expression

Mammalian

Mutation

Y1162F

Available Since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only
HIR Y1163F

Plasmid

#24053

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Expression

Mammalian

Mutation

Y1163F

Available Since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only
pEU3-NII-GLICNot-C-His

Plasmid

#140187

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

6xHis and GST

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMJ915v2+Nterm Spe1 +Cterm Age1 site

Plasmid

#88914

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pTH

Plasmid

#56659

Purpose

Dual-expression vector for P. tricornutum

Depositor

Philip Weyman

Article

Weyman et al Plant Biotechnol J. 2014 Oct 10. doi: 10

Insert

KanR

Use

Synthetic Biology; Diatom expression

Available Since

Oct. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pVAX-Help-SIINFEKL

Plasmid

#141350

Purpose

DNA vaccination plasmid containing SIINFEKL and several CD4+ T cell epitopes

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

HELP-SIINFEKL-KDEL

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly-RpS12

Plasmid

#71504

Purpose

flySTARR-seq screening vector with RpS12 core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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LLP743_L2pV1_OR_B3RT_FRT_Rluc_0I_lacZ

Plasmid

#192387

Purpose

To act as the 0-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 IL2

Plasmid

#67053

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL2 (IL2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 RUVBL1

Plasmid

#67091

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

RUVBL1 (RUVBL1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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EK0302 SFFV-mCherry-SG(Bdnf.s2)SG-EGFP (FLP-IN)

Plasmid

#191179

Purpose

Negative control sensor in human cells.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s2:EGFP (negative control)

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-LbCas12a

Plasmid

#213743

Purpose

For circular RNA-mediated prime editor using LbCas12a in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

LbCas12a

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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TAL2385

Plasmid

#36794

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BRCA1-TALEN_Right (Brca1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
Cherry-Myo1b

Plasmid

#187366

Purpose

Expresses rat myosin 1b isoform b domain labelled with Cherry

Depositor

Evelyne Coudrier

Article

Iuliano et al J Cell Biol. 2018 Jun 4;217(6):2033-2046

Insert

myosin 1b isoform b (Myo1b Rat)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pX601‐mhCMV‐miniABEmax‐N2‐ E53ogRNA

Plasmid

#187065

Purpose

Gp41-1 Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

miniABEmax-Gp41-1

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mhCMV

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pX601‐mhCMV‐ABEmaxNGA‐C2‐ E53ogRNA

Plasmid

#187066

Purpose

Gp41-1 Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

Gp41-1-ABEmaxNGA

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mhCMV

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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CP1300-ABEmax

Plasmid

#119810

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA5

Plasmid

#64985

Purpose

Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, D160N, D165A, K19…

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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YPRCΔ15α1

Plasmid

#101712

Purpose

GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Type

Empty backbone

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST-hPDIb′a′

Plasmid

#60556

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Article

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Tags

His6-hPDIb'a' tag

Expression

Bacterial

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pOCC114

Plasmid

#118904

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6, cleavable with TEV protease, two glycines

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET16b-Trx C35S

Plasmid

#36947

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin-1 C35S mutation (TXN Human)

Tags

His tag

Expression

Bacterial

Mutation

cys 35 to serine

Promoter

T7

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
pML3009

Plasmid

#193256

Purpose

Gamma-aminobutyric acid sensor expressing eYFP reporter

Depositor

Lauren Andrews

Article

Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

Inserts

gabR
eYFP

Use

Synthetic Biology

Promoter

synthetic promoter P2 and synthetic promoter PGab…

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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