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pHH0103_CRK-2/2-SV2/2

Plasmid

#91343

Purpose

Protein expression and purification of human SH3 domain construct CRK-2/2-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CRK-2/2-SV2/2 (CRK Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES:mTREK-1(K271Q)

Plasmid

#133273

Purpose

Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tag

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Tags

HA

Expression

Mammalian and Yeast

Mutation

K271Q

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SRC-1/1-IS1/2

Plasmid

#91349

Purpose

Protein expression and purification of human SH3 domain construct SRC-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRC-1/1-IS1/2 (SRC Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Mouse GFP-PRMT7 LDIG(69-72)AAAA

Plasmid

#65044

Purpose

Expresses Catalytically inactive GFP-PRMT7 LDIG(69-72)AAAA mutant

Depositor

Steven Clarke

Article

Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

Insert

PRMT7 (Prmt7 Mouse)

Use

Bacterial propagation

Tags

GFP

Expression

Mammalian

Mutation

L69A D70A I71A G72A

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pHH0103_ASAP1-1/1

Plasmid

#91501

Purpose

Protein expression and purification of human SH3 domain construct ASAP1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ASAP1-1/1 (ASAP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCSIIneo-TRE-hPhyB621- mCherry-HRasCT

Plasmid

#139482

Purpose

Expresses PhyB621-mCherry-HRasCT in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

PhyB621 (PHYB Mustard Weed)

Use

Lentiviral

Tags

mCherry-HRasCT

Expression

Mammalian

Mutation

G229L and deletion of aa Y at position 235 in mch…

Promoter

Tet responsible element

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_DNMBP-6/6

Plasmid

#91489

Purpose

Protein expression and purification of human SH3 domain construct DNMBP-6/6

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DNMBP-6/6 (DNMBP Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_KALRN-1/4

Plasmid

#91283

Purpose

Protein expression and purification of human SH3 domain construct KALRN-1/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

KALRN-1/4 (KALRN Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN1-1/5-IS2/3

Plasmid

#91318

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-1/5-IS2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-1/5-IS2/3 (ITSN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_LASP1-1/1-SV2/2

Plasmid

#91392

Purpose

Protein expression and purification of human SH3 domain construct LASP1-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LASP1-1/1-SV2/2 (LASP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MIA3-1/1

Plasmid

#91327

Purpose

Protein expression and purification of human SH3 domain construct MIA3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MIA3-1/1 (MIA3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_PLCG1-1/1

Plasmid

#91540

Purpose

Protein expression and purification of human SH3 domain construct PLCG1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PLCG1-1/1 (PLCG1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBI121::chitGFP5:AQ

Plasmid

#240450

Purpose

Expression of indicator protein fusion (soluble modified GFP5 & Aequorin) for monitoring calcium concentrations in cell walls of higher plants. See Resource Information.

Depositor

Christoph Plieth

Article

Gao et al Plant Physiol. 2004 Mar;134(3):898-908.

Insert

Fusion of Chitinase signal, smGFP5, and Aequorin

Expression

Bacterial and Plant

Mutation

GFP5 for expression plants (PMID 9122158); Solubl…

Promoter

Cauliflower mosaic virus 35S promoter (GenBank AJ…

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-InPGrp

Plasmid

#236002

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to lysosome surface.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Lyso-InPGrp (Cyth3 Rat, Synthetic)

Tags

Cerulean, Full-length LAMP1, and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-WIPI2d IDR (364-425aa)

Plasmid

#223790

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI2d IDR (WIPI2 Human)

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Mutation

aa 364-425 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-WIPI2d (K87A/K88A)

Plasmid

#223751

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI2d (WIPI2 Human)

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Mutation

K87A/K88A

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)

Plasmid

#223724

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FIP200-CTR (RB1CC1 Human)

Tags

6xHis-TEV-EGFP

Expression

Bacterial

Mutation

aa 1429-1591 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-LC3C

Plasmid

#223727

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

LC3C (MAP1LC3C Human)

Tags

GST

Expression

Bacterial

Mutation

aa 2-126 only, with Glycine exposed

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_mCherry-ATG13 (WT)

Plasmid

#223735

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 (ATG13 Human)

Tags

mCherry

Expression

Mammalian

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_GST-TEV-WIPI1

Plasmid

#223798

Purpose

Expression of recombinant protein for purification

Depositor

James Hurley, Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI1 (WIPI1 Human)

Tags

GST-TEV

Expression

Mammalian

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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