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pAAV-hSyn-GRAB_CRF1.0

Plasmid

#208658

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0

Use

AAV

Promoter

hSyn

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDragon-Ctgf

Plasmid

#155015

Purpose

For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to connective tissue growth factor in the presence of Cre and (r)tTA activity.

Depositor

Alberto Rosello-Diez

Article

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Insert

Ccn2 (Ccn2 Mouse)

Use

Mouse Targeting

Mutation

M24T (please see depositor comments)

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gad65-WPRE-HGHpA

Plasmid

#184631

Purpose

Expresses Gad65, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gad65 (Gad2 Mouse)

Use

AAV

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Nsp4-mCherry

Plasmid

#165132

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp4 (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-H2BmGreenLantern

Plasmid

#177332

Purpose

To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

Depositor

Murray Blackmore, Gregory Petsko, Pantelis Tsoulfas

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

H2B (Hist1h2bq Rat)

Use

AAV

Tags

mGreenLantern

Expression

Mammalian

Mutation

This fusion protein was optimized to the Human co…

Promoter

CAG

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Orf3a-mCherry

Plasmid

#165138

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Orf3a (ORF3a codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry C1 Omp25

Plasmid

#157758

Purpose

Expression of mcherry-labelled mitochondrial outer membrane protein 25 in cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

SYNJ2bp (SYNJ2BP Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TREtight-mTagBFP2-B19G

Plasmid

#100799

Purpose

helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

Depositor

Ian Wickersham

Article

Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

Has Service

AAV1

Insert

mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)

Use

AAV

Expression

Mammalian

Promoter

TREtight

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-AIM2

Plasmid

#73958

Purpose

Express c-Myc-tagged human AIM2 in mammalian cells

Depositor

Christian Stehlik

Article

Khare et al Nat Immunol. 2014 Apr;15(4):343-53. doi:

Insert

absent in melanoma 2 (AIM2) (AIM2 Human)

Tags

c-myc

Expression

Mammalian

Promoter

CMV iE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pFN18A-HaloTag-Biotin

Plasmid

#206039

Purpose

HaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTag

Depositor

Sergi Garcia-Manyes

Article

Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

Insert

Talin R3-IVVI (Tln1 Mouse)

Tags

10 x His-AviTag and Halo-Tag

Expression

Bacterial

Mutation

Threonine 809 to Isoleucine, Threonine 833 to Val…

Promoter

T7

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VP64-Puro

Plasmid

#99371

Purpose

3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-VP64-T2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-Ikkb-FLAG WT

Plasmid

#23298

Depositor

Warner Greene

Article

Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

Insert

Ikkbeta (IKBKB Human)

Tags

Flag

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-his-BACH1 K52R

Plasmid

#17643

Depositor

Ronny Drapkin

Article

Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

Insert

BACH1 (BRIP1 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

The lysine residue at position 52 was changed to …

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only
PCMV-Tag4A-MMP8 (E138Q)

Plasmid

#29549

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

E138Q

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pMR506-EF1a-H2B-EGFP

Plasmid

#186627

Purpose

Expresses nuclear-localised EGFP under EF1a promoter. For insertion of genetic barcodes into 3'UTR of EGFP.

Depositor

Jonas Frisén

Article

Ratz et al Nat Neurosci. 2022 Mar;25(3):285-294. do

Insert

EF1a (EEF1A1 Synthetic)

Use

Lentiviral

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-HA_N-human Prkd2

Plasmid

#137850

Purpose

Express Prkd2 in mammalian cells

Depositor

Bruce Beutler

Article

Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

Insert

Protein kinase d2 (PRKD2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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Nsp6-mCherry

Plasmid

#165133

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp6 (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6.3 GCN1 R2312A-3xFlag_IRES-iRFP

Plasmid

#198389

Purpose

GCN1 R2312A expresson

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

GCN1 (GCN1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

R2312A mutation in RWDBD (RWD binding domain)

Promoter

CMV

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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sc-WDR24-SEH1L

Plasmid

#237418

Purpose

Transient expression of human WDR24-SEH1L "single chain" in the GATOR2 complex

Depositor

David Sabatini

Article

Valenstein et al Nature. 2025 Aug 20. doi: 10.1038/s41586

Insert

WDR24-SEH1L fusion (WDR24, SEH1L Human)

Expression

Mammalian

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b(+)_rPD-L1nb

Plasmid

#226715

Purpose

Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Recombinant PD-L1 nanobody

Tags

HA tag and Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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