We narrowed to 6,115 results for: arl

1. pENTR CMVie-Lifeact-mTagBFP2 L1-R5

   Plasmid

   #84388

   Purpose

   Multisite Gateway entry vector for expression of mTagBFP2-tagged Lifeact in cells including neurons

   Depositor

   Ghassan Mouneimne

   Insert

   Lifeact
   

   Use

   Gateway

   Tags

   mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV immediate early

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR CMVie-Lifeact-mCardinal L1-R5

   Plasmid

   #84392

   Purpose

   Multisite Gateway entry vector for expression of mCardinal-tagged Lifeact in cells including neurons

   Depositor

   Ghassan Mouneimne

   Insert

   Lifeact
   

   Use

   Gateway

   Tags

   mCardinal

   Expression

   Mammalian

   Promoter

   CMV immediate early

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCMV3

   Plasmid

   #85711

   Purpose

   Expression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequences

   Depositor

   Ulrich Koszinowski, Martin Messerle

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   MIEPmouse CMV MIEP

   Available Since

   April 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-fDIO-EYFP-WPRE

   Plasmid

   #154870

   Purpose

   Human synapsin-1 promoter; Flp-dependent EYFP

   Depositor

   Ulrik Gether

   Insert

   EYFP
   

   Use

   AAV and Mouse Targeting; Flp-frt

   Expression

   Mammalian

   Promoter

   Human synapsin promoter

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabepuro-PDGFB

   Plasmid

   #189611

   Purpose

   Retroviral expression vector for PDGFB. stimulates fibroblastic proliferation (Tchenio T, unpublished, 2008)

   Depositor

   Thierry Tchenio

   Insert

   PDGFB (PDGFB Human)
   

   Use

   Retroviral

   Promoter

   SV40 early promoter

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-nEF Con/Fon DREADD Gq-mCherry

   Plasmid

   #183532

   Purpose

   INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp

   Depositor

   Karl Deisseroth

   Insert

   DREADD Gq-mCherry
   

   Use

   AAV

   Mutation

   None

   Promoter

   nEF

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Plenti-CMV-MCS-RFP-SV-puro

   Plasmid

   #109377

   Purpose

   This plasmid expresses dsRed2 and puromycin resistance

   Depositor

   Jonathan Garlick, Behzad Gerami-Naini

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   dsRed2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pInt_attP1_sacB_kanR

   Plasmid

   #205301

   Purpose

   Integrating plasmid for ORBIT in E. coli with sacB for counter selection. Used for markerless or scarless modifications.

   Depositor

   Scott Saunders

   Article

   Saunders et al bioRxiv 2023

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. tetO-GFPnls-mPGK-rtTA-IRES-Puro

   Plasmid

   #69545

   Purpose

   Tet-On system expressing GFP tagged with 2x nuclear localization signals.

   Depositor

   Charles Gersbach

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   2x nuclear localization sequence and T2A "sk…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-GFAP-hChR2(H134R)-EYFP

    Plasmid

    #27054

    Purpose

    AAV expression of humanized ChR2(H134R) fused to EYFP, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    GFAP

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-GFAP-hChR2(H134R)-mCherry

    Plasmid

    #27055

    Purpose

    AAV expression of humanized ChR2(H134R) fused to mCherry, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    GFAP

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-nEF Coff/Fon DREADD Gq-mCherry

    Plasmid

    #183534

    Purpose

    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Flp but not Cre

    Depositor

    Karl Deisseroth

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV

    Mutation

    None

    Promoter

    nEF

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOpen-T4gene30

    Plasmid

    #165554

    Purpose

    Ligase derived from T4 bacteriophage expressed in the early stage of lytic development. Has been implicated in T4 DNA synthesis and genetic recombination. May also play a role in T4 DNA repair.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    T4-DNA Ligase
    

    Use

    Synthetic Biology

    Available Since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pAAV-nEF Con/Foff DREADD Gq-mCherry

    Plasmid

    #183533

    Purpose

    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre but not Flp

    Depositor

    Karl Deisseroth

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV

    Mutation

    None

    Promoter

    nEF

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabepuro-IL11

    Plasmid

    #189609

    Purpose

    Retroviral expression vector for IL11

    Depositor

    Thierry Tchenio

    Insert

    IL11 (IL11 Human)
    

    Use

    Retroviral

    Promoter

    SV40 early promoter

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJF3H

    Plasmid

    #63897

    Purpose

    For phage display of cDNA libraries

    Depositor

    Carlos Barbas

    Article

    Barbas et al Cold Spring Harbor, NY: Cold Spring Harb

    Insert

    cDNA cloning region
    

    Use

    Phage display

    Tags

    Fos

    Expression

    Bacterial

    Promoter

    lacZ

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-hChR2(H134R)-mCherry

    Plasmid

    #26976

    Purpose

    AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Has Service

    AAV5, AAV8, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    hSyn

    Available Since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-HA-SOX4

    Plasmid

    #110367

    Purpose

    Mammalian expression of HA-tagged SOX4

    Depositor

    Carlos Moreno

    Insert

    SOX4 (SOX4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH

    Plasmid

    #100050

    Purpose

    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV9

    Insert

    hChR2(E123T/H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    humanized, E123T/H134R

    Promoter

    CaMKIIa

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-double floxed-EYFP-WPRE-HGHpA

    Plasmid

    #20296

    Purpose

    Cre-activated AAV expression of EYFP

    Depositor

    Karl Deisseroth

    Insert

    EYFP
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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