We narrowed to 75,518 results for: LAS

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lentiCRISPRv2-Blast-mU6

Plasmid

#206806

Purpose

pLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassette

Depositor

Jolanda Van Leeuwen

Article

Unlu et al Genome Med. 2023 Oct 12;15(1):78. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHH29_32 series plasmid

Plasmid

#120901

Purpose

Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

Inserts

Ec-TTL-P109
sRNA-A targeting sequence
RBS TIR=974 of GFPop1 gene
GFPop1
RBS TIR=6474 of ECF32mut gene
ECF32mut
T7 terminator
resource competitor

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCas9-sgCh2-2-Blast

Plasmid

#199645

Purpose

Expresses Cas9 and sgRNA control guide targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-ERK2 D321N

Plasmid

#53173

Purpose

Expresses HA tagged human ERK2 D321N from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human, Mustard Weed)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

D321N

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pWZL blast HA goosecoid

Plasmid

#14096

Depositor

Bob Weinberg

Article

Hartwell et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

Insert

goosecoid (Gsc Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast shGAC

Plasmid

#110410

Purpose

shGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.

Depositor

Sandra Martha Gomes Dias

Insert

GLS glutaminase (GLS Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 (RNA Pol III)

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mCherry

Plasmid

#179789

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mCherry

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 blast EF1a

Plasmid

#206223

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR-v2-Blast-Puro

Plasmid

#167186

Purpose

Lentiviral expression of sgRNA with blasticidin and puromycin resistance genes

Depositor

Yin Shen

Article

Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

Insert

Blasticidin S deaminase

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLX304 LacZ-V5 blast

Plasmid

#83099

Purpose

Lentiviral vector for constitutive expression of LacZ with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

2x V5 tag

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLenti-Blast-sgSIK2

Plasmid

#138708

Purpose

Expresses a human SIK2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK2 human (SIK2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgCRTC2-1

Plasmid

#138700

Purpose

Expresses a human CRTC2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgCRTC2 human (CRTC2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgCRTC2-2

Plasmid

#138701

Purpose

Expresses a human CRTC2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgCRTC2 human (CRTC2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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MSCVpic2blast-EGFP-FF2

Plasmid

#164923

Purpose

Expresses EGFP cDNA and FF2 shRNA in mammalian cells

Depositor

Ji Luo

Article

Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

Inserts

Enhanced green fluorescent protein
Neuropeptide FF receptor 2 (NPFFR2 Human)

Use

Retroviral

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mScarlet

Plasmid

#179818

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mScarletI

Plasmid

#201442

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-MEK1 C121S

Plasmid

#53165

Purpose

Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C121S

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
FFiBlast MSCV Puro

Plasmid

#68464

Purpose

Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Blasticidin Resistance

Use

Cre/Lox and Retroviral

Expression

Mammalian

Mutation

Inverted

Promoter

LTR

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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BGGLO QPCR PLASMID

Plasmid

#79905

Purpose

Encodes partial human gamma- and beta-globing cDNA for use as QPCR standard as published in PMID: 14592835..

Depositor

Jeffery Miller

Article

Bhanu et al Blood. 2004 Mar 1;103(5):1929-33. Epub 2

Insert

Human partial HBG and HBB gene

Promoter

No promoter

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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MS2392.EF1a.SLC6A1.WT.P2A.BLAST.JL.p159

Plasmid

#170170

Purpose

Lentiviral Expression of EF1a.SLC6A1.WT

Depositor

Christopher Moxham

Insert

SLC6A1 (SLC6A1 Human)

Use

Lentiviral

Mutation

n/a

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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