We narrowed to 29,560 results for: URE

1. lenti sgRNA(MS2)_zeo backbone

   Plasmid

   #61427

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti sgRNA(MS2)_puro backbone

   Plasmid

   #73795

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV CMV-dSaCas9-KRAB-bGHpA

   Plasmid

   #106219

   Purpose

   AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

   Insert

   dead S. aureus Cas9 KRAB
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   CMV

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

   Plasmid

   #61594

   Purpose

   A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Insert

   hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV hUbC-dSaCas9-KRAB-T2A-PuroR

   Plasmid

   #106249

   Purpose

   Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

   Insert

   dead S. aureus Cas9 KRAB T2A PuroR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA-NLS and NLS-KRAB

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   hUbC

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. DTECT - Plasmid for Standard Curve

   Plasmid

   #139333

   Purpose

   This plasmid is used to generate the unique standard curve required to quantify the capture of genomic signature using DTECT

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   DTECT ligation product
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223-NPR2

   Plasmid

   #23479

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   NPR2 (NPR2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   isoform missing aa905-962

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. MS2-P65-HSF1_GFP

   Plasmid

   #61423

   Purpose

   Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Insert

   MS2(N55K)-P65-HSF1_2A_GFP (HSF1 Synthetic, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N55K in MS2

   Promoter

   EF1A

   Available since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. SV.signed

   Plasmid

   #133933

   Purpose

   To validate the use of digital signatures in DNA.

   Depositor

   Jean Peccoud

   Insert

   lacZ and digital signature cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti MS2-P65-HSF1_Hygro

    Plasmid

    #61426

    Purpose

    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Insert

    MS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1A

    Available since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSaGuide

    Plasmid

    #64710

    Purpose

    Cloning and expression of Staphylococcus aureus guide RNA

    Depositor

    Kiran Musunuru

    Insert

    Staphylococcus aureus guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK554

    Plasmid

    #72453

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pet30b-7M SrtA

    Plasmid

    #51141

    Purpose

    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Insert

    S.aureus SrtA 7M
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, E105K, E108Q, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLI50

    Plasmid

    #13573

    Depositor

    Chia Lee

    Article

    Lee et al Gene. 1991 Jul 15. 103(1):101-5.

    Type

    Empty backbone

    Use

    S. aureus-e. coli shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAIP4_5IHW

    Plasmid

    #234148

    Purpose

    Heterologous protein expression of serine-aspartate repeat-containing protein E in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5IHW
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pAIP4_2OKT

    Plasmid

    #234149

    Purpose

    Heterologous protein expression of o-succinylbenzoate synthase (OSB synthase) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2OKT
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pJK550

    Plasmid

    #72450

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    inserted Val2

    Promoter

    T7

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK322

    Plasmid

    #73494

    Purpose

    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide mutase

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    n/a

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK684

    Plasmid

    #71638

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Val mutant (TdPurE2-S38V)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-38 to valine

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK685

    Plasmid

    #71639

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asn mutant (TdPurE2-S38N)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-38 to asparagine

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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