Addgene

We narrowed to 17,042 results for: URE

Showing: 421 - 440 of 17042 results

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pJK510

Plasmid

#71600

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp165>Phe mutant (AaPurE1wwf-H59N)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Use

Tags

Expression

Bacterial

Mutation

changes histidine-59 to asparagine, tryptophan-16…

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Lifeact-EGFP

Plasmid

#58470

Purpose

Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

Lifeact

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pKOR1

Plasmid

#133446

Purpose

Escherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selection

Depositor

Olaf Schneewind, Taeok Bae

Article

Bae et al Plasmid. 2006 Jan;55(1):58-63. doi: 10.1

Type

Empty backbone

Use

E.coli/s.aureus shuttle vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ0135

Plasmid

#11412

Depositor

Sung-Hou Kim

Article

Lai et al Structure. 2000 Aug 15. 8(8):897-904.

Insert

RNase HII (MJ_RS00700 M. jannaschii)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pCasY1

Plasmid

#87687

Purpose

p15A plasmid expressing CasY1

Depositor

Jennifer Doudna

Article

Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

Insert

CasY1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pJK559

Plasmid

#71603

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-Y154F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Use

Tags

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, tyrosine-…

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK562

Plasmid

#71604

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Leu/Trp165>Phe mutant (AaPurE1fwf-Y154L)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Use

Tags

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, tyrosine-…

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK604

Plasmid

#71610

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe mutant (AaPurE1fww-H59N,Y154F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Use

Tags

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, histidine…

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK664

Plasmid

#71613

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-H59N,Y154F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Use

Tags

Expression

Bacterial

Mutation

changes histidine-59 to asparagine, tyrosine-154 …

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK666

Plasmid

#71615

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Use

Tags

Expression

Bacterial

Mutation

changes histidine-59 to asparagine, tryptophan-97…

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK563

Plasmid

#71689

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Ile/Trp165>Phe mutant (AaPurE1fwf-Y154I)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Use

Tags

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, tyrosine-…

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK508

Plasmid

#71598

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp165>Phe mutant (AaPurE1fwf-H59N)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Use

Tags

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, histidine…

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSR1058

Plasmid

#208743

Purpose

Characterization plasmid for PHbs (sfgfp expressed from PHbs on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PHbs

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PHbs

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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GFP_CD47_SU

Plasmid

#65473

Purpose

GFP tagged CD47 with its own short 3'UTR

Depositor

Christine Mayr

Article

Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature

Inserts

Signal peptide of CD47
GFP
CD47 coding region continued and short 3’UTR.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pet26-MBP-ELIC

Plasmid

#39239

Depositor

Raimund Dutzler

Article

Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

Insert

Eriwinia chrysanthemi - pentameric ligand gated ion channel

Use

Tags

His-10 and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pCasX1

Plasmid

#87685

Purpose

p15A plasmid expressing CasX locus from Deltaproteobacteria

Depositor

Jennifer Doudna

Article

Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

Insert

CasX1

Use

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-pIgR cDNA

Plasmid

#12109

Depositor

Pamela Bjorkman

Article

Hamburger et al Structure. 2004 Nov . 12(11):1925-35.

Insert

human polymeric immunoglobulin receptor (PIGR Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
EC.SurA.pTYB1.a21-428.wt

Plasmid

#12606

Depositor

David McKay

Article

Bitto et al Structure. 2002 Nov . 10(11):1489-98.

Insert

Escherichia coli surA (surA E. coli)

Use

Tags

intein

Expression

Bacterial

Mutation

wild type

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pSR1048

Plasmid

#208738

Purpose

Characterization plasmid for PRelA (sfgfp expressed from PRelA on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PRelA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PRelA

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET21b - S. thermophilus DltB

Plasmid

#132607

Purpose

expresses S. thermophilus DltB in E. coli

Depositor

Wenqing Xu

Article

Ma et al Nature. 2018 Oct;562(7726):286-290. doi:

Insert

Dltb

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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