We narrowed to 1,931 results for: cas9 expression vector

1. pENTR-L3-Csy4-Cas9n-EGFP-L2

   Plasmid

   #141046

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Csy4-2A-Cas9n-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

   Plasmid

   #141052

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   eSpCas9(1.1)-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-L3-Csy4-Cas9-EGFP-L2

   Plasmid

   #141045

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Csy4-2A-Cas9-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pENTR-L3-eSpCas9(1.1)-2A-EGFP-L2

   Plasmid

   #141051

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   eSpCas9(1.1)-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-L3-Csy4-FokI-dCas9-L2

   Plasmid

   #141042

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Csy4-2A-FokI-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-L3-SpCas9-HF4-2A-EGFP-L2

   Plasmid

   #141049

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   SpCas9-HF4-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-L3-SpCas9-HF2-2A-EGFP-L2

   Plasmid

   #141050

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   SpCas9-HF2-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-EF1alpha-VP64-dCas9-p300_Blast

   Plasmid

   #192654

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-EF1alpha-VP64-dCas9-VP64_Blast

   Plasmid

   #192650

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

    Plasmid

    #190794

    Purpose

    Template vector to amplify pieces for creating multiplex sgRNA-arrays.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA-scaffold only
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23117

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB_Mut(D1399Y)_p300_core-dCas9-EGFP_hygro

    Plasmid

    #226427

    Purpose

    PiggyBac transposon vector containing a dox inducible mutant p300 core-dCas9 fusion protein (p300 core mutation: D1399Y) and EGFP reporter. Hygromycin selection marker.

    Depositor

    Alon Goren

    Inserts

    Mutant p300 core-dCas9-EGFP fusion protein
    rtTA3-IRES-Hygro
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    HA tag, Mutant p300 core, and P2A-EGFP

    Expression

    Mammalian

    Mutation

    p300 core mutation (D1399Y)

    Promoter

    Dox inducible minimal CMV and UbC Promoter

    Available since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCjeCas9

    Plasmid

    #172215

    Purpose

    Expresses Campylobacter jejuni Cas9 (CjeCas9) in bacterial cells

    Depositor

    John van der Oost

    Article

    Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

    Insert

    Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
    

    Use

    Tags

    Histag

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCjedCas9

    Plasmid

    #172216

    Purpose

    Expresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cells

    Depositor

    John van der Oost

    Article

    Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

    Insert

    catalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
    

    Use

    Tags

    Histag

    Expression

    Mutation

    changed Asp 8 to Ala and His 559 to Ala

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-SpCas9-GFP-U6-gRNA

    Plasmid

    #79144

    Purpose

    All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

    Depositor

    Jizhong Zou

    Article

    Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. xCas9(3.7)-ABE(7.10)

    Plasmid

    #108382

    Purpose

    Mammalian expression vector for xCas9(3.7)-ABE

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.7)-ABE(7.10)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. xCas9(3.6)-ABE(7.10)

    Plasmid

    #108387

    Purpose

    Mammalian expression vector for xCas9(3.6)-ABE

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.6)-ABE(7.10)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSH231-EFS-Cas9-BlastR

    Plasmid

    #115146

    Purpose

    Safe harbor site 231 knock-in vector with Cas9-BlastR expression cassette

    Depositor

    Raymond Monnat, Michael Phelps

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    Cas9-T2A-BlastR
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-SauriCas9-Puro

    Plasmid

    #135965

    Purpose

    Expresses SauriCas9 and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL3.7-hSt3Cas9 (pXK-1721)

    Plasmid

    #208828

    Purpose

    CRISPR/hSt3Cas9 expression vector for Animal gene editing, harboring the humanized St3Cas9 gene and the corresponding optimized SP1.gRNA. Key Construct: mU6- SP1.gRNA-CMV-hSt3Cas9.

    Depositor

    Kun Xu

    Article

    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99

    Type

    Empty backbone

    Use

    CRISPR; Stcas9

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ1658-dCas9-EGFP

    Plasmid

    #51023

    Purpose

    Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)

    Depositor

    Bo Huang, Stanley Qi

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    dCas9 fuse to EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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