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We narrowed to 11,728 results for: cel.2;

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pCMV6-AN-DDK_UBM1*2* POLI

Plasmid

#131232

Purpose

Mammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domains

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains inactivating mutations in both ubiquitin…

Promoter

CMV enhancer + CMV promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

Plasmid

#172066

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-CRISPRv2-sgIl1r1_#2-hygro

Plasmid

#231990

Purpose

Knockout mouse Il1r1

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance (Il1r1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-100RPU.2-ETN-BGHpA-cHS4-100RPU.2-ETN-lox2272

Plasmid

#154834

Purpose

Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of etanercept

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Inserts

ETN
ETN

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

100RPU.2

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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PGK1p-Csy4-pA (Construct 2)

Plasmid

#55196

Purpose

Expresses Csy4 in Mammalian cells for site-specific RNA processing

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

Csy4/Cas6

Use

Synthetic Biology

Tags

6xHIS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro shSlp4-a #2

Plasmid

#40070

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp4-a shRNA-2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA4 CoV-2 nsp1 3xFLAG

Plasmid

#176057

Purpose

Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHMTc-FBF-2 (121-632)

Plasmid

#218664

Purpose

Expression of MBP:6xHIS:FBF-2(121-632)

Depositor

Judith Kimble

Article

Carrick et al Dev Cell. 2024 Mar 11;59(5):661-675.e7.

Insert

fbf-2 (fbf-2 Nematode)

Use

Tags

MBP-6xHIS

Expression

Bacterial

Mutation

amino acids 121-632 only

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272

Plasmid

#154829

Purpose

Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of erythropoietin

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Inserts

coEPO
coEPO

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Codon-optimized (Cricetulus griseus)

Promoter

100RPU.2

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75235

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75239

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Helios-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75247

Purpose

CRISPR/Cas9 NICKASE plasmid against human Helios (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Helios (IKZF2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shPRDM14-2

Plasmid

#193695

Purpose

Constitutive lentiviral expression of PRDM14 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-ML1N*2

Plasmid

#67665

Purpose

Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-ML1N*2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Notch1 #2 GFP

Plasmid

#106951

Purpose

Lentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

gRNA targeting Notch1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shSLC2A1-2

Plasmid

#193703

Purpose

Constitutive lentiviral expression of SLC2A1 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

SLC2A1 (SLC2A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shYAP1-2

Plasmid

#193693

Purpose

Constitutive lentiviral expression of YAP1 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNkx2-1#2/Cre

Plasmid

#193222

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Nkx2-1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNkx2-1#2 (Nkx2-1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shCALM2-2

Plasmid

#193700

Purpose

Constitutive lentiviral expression of CALM2 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

CALM2 (CALM2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2 TetO(fl)-2

Plasmid

#24416

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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