We narrowed to 2,375 results for: pcas9

1. pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL

   Plasmid

   #107305

   Purpose

   Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2x NLS and FKBP

   Expression

   Mammalian

   Mutation

   R1335K and fused to FKBP

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-Flag-eSpCas9-H840A

   Plasmid

   #80454

   Purpose

   enhanced SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   ehSpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9-Puro HLAG-2B-sgRNA

   Plasmid

   #182552

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2B-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH

   Plasmid

   #148855

   Purpose

   Mammalian Expression of HsAGO3-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsAGO3-sgRNA (AGO3 S.pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-SpCas9_sgRNA-site2 (RTW448)

   Plasmid

   #160137

   Purpose

   T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX544 SpCas9(del1098-1368)

   Plasmid

   #58889

   Purpose

   Truncated form of SpCas9 without C' domain

   Depositor

   Feng Zhang

   Article

   Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   del amino acids 1098-1368

   Promoter

   Available Since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-dHeFSpCas9-VP64-6xHis

   Plasmid

   #92119

   Purpose

   Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   T7

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAWp63-clone32 (Opti-SpCas9)

   Plasmid

   #131736

   Purpose

   Expresses Opti-SpCas9 in mammalian cells

   Depositor

   Alan Siu Lun Wong

   Article

   Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

   Insert

   Opti-SpCas9
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   R661A+K1003H

   Promoter

   EFS

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

   Plasmid

   #98976

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 1 and Cas9(N863A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-FLAG-eSpCas9-plus

    Plasmid

    #126774

    Purpose

    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-Puro-Exon4-PAFAH1B1

    Plasmid

    #92433

    Purpose

    expresses Cas9 and gRNA targeting PAFAH1B1

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

    Insert

    PAFAH1B1 (PAFAH1B1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

    Plasmid

    #141052

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    eSpCas9(1.1)-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX543 SpCas9(del175-307)

    Plasmid

    #58888

    Purpose

    Truncated form of SpCas9 without REC2 domain

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    del amino acids 175-307

    Promoter

    Available Since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-Flag-HeFSpCas9-H840A

    Plasmid

    #80456

    Purpose

    high fidelity enhanced SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    HeFSpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-2)

    Plasmid

    #236613

    Purpose

    Knockouts ANKRD11 in human cells

    Depositor

    Tadashi Nakagawa

    Article

    Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

    Insert

    ANKRD11 (ANKRD11 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-1)

    Plasmid

    #236612

    Purpose

    Knockouts ANKRD11 in human cells

    Depositor

    Tadashi Nakagawa

    Article

    Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

    Insert

    ANKRD11 (ANKRD11 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9(BB)-2A-puro-mAnkrd11 (intron 3)

    Plasmid

    #236608

    Purpose

    Knockouts Ankrd11 in mouse cells

    Depositor

    Tadashi Nakagawa

    Article

    Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

    Insert

    Ankrd11 (Ankrd11 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-U6-MYC-Guide-hSpCas9

    Plasmid

    #228574

    Purpose

    Expression of human MYC gRNA; CRISPR mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    MYC gRNA (MYC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-U6-E690_guide-CBh-hSpCas9

    Plasmid

    #188545

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-U6-W308_guide-CBh-hSpCas9

    Plasmid

    #188546

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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