We narrowed to 10,383 results for: Coli

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pBAD-pnGFP-Ultra

Plasmid

#157923

Purpose

Peroxynitrite sensor pnGFP-Ultra in E. coli (use with pEvol-BoF)

Depositor

Huiwang Ai

Article

Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

Insert

pnGFP-Ultra, a high performance peroxynitrite sensor

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL80016

Plasmid

#50332

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS no stop codon, beta-glucuronidase (E. coli) with two introns

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFLIP43

Plasmid

#65618

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-edAFPt9-attR1 and attR2-edCer-cMyc

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pIA1408

Plasmid

#172405

Purpose

Expression of SARS-COV-2 Nsp12 D760A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Mutation

D760A

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_P3-ehaA_LacI

Plasmid

#186315

Purpose

Plasmid for inducible E coli expression of the P3 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

P3-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_AP4-ehaA_LacI

Plasmid

#186316

Purpose

Plasmid for inducible E coli expression of the AP4 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

AP4-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pReg-ROTU4

Plasmid

#190669

Purpose

Expresses an evolved rotundine biosensor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

ROTU4

Use

Synthetic Biology

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pGfOMT1-GEN4

Plasmid

#190612

Purpose

Expresses an evolved methyltransferase for tetrahydropapaverine biosynthesis in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

GfOMT-GEN4

Use

Synthetic Biology

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pReg-GLAU4

Plasmid

#190667

Purpose

Expresses an evolved glaucine biosensor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

GLAU4

Use

Synthetic Biology

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pReg-PAP4

Plasmid

#190668

Purpose

Expresses an evolved papaverine biosensor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

PAP4

Use

Synthetic Biology

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pReg-NOS4

Plasmid

#190670

Purpose

Expresses an evolved noscapine biosensor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

NOS4

Use

Synthetic Biology

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-MseLig1

Plasmid

#107071

Purpose

Expresses M. sedula DNA Ligase 1 in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

DNA ligase 1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pVY-3 sfGFP-tag3

Plasmid

#194618

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

sfGFP-K(GGSKKRKKR)3

Tags

KGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFLIP37

Plasmid

#65505

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-Cit-attR1 and attR2-Cer-cMyc

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pET-G2K

Plasmid

#70169

Purpose

bacterial expression of G2K (d-glycerate 2-kinase)

Depositor

George Reed, Paul Sims

Article

et al Journal of Molecular Catalysis B: Enzyma

Insert

garK/G2K

Tags

6xHIS and V5

Expression

Bacterial

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-HT_RcCHAD

Plasmid

#127782

Purpose

Expresses RcCHAD in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

RcCHAD

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAtLCYbSK

Plasmid

#53588

Purpose

Produces an active Arabidopsis thaliana lycopene beta-cyclase enzyme in E. coli. Also known as pAt9SK.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

Insert

lcyB (LYC Mustard Weed)

Expression

Bacterial

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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MS2-ecDHFR

Plasmid

#65859

Purpose

Expresses RNA-binding protein MS2 with eDHFR fusion tag

Depositor

Aaron Hoskins

Article

Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

Inserts

MS2
E. coli Dihydrofolate Reductase (folA )

Tags

HA and NLS

Expression

Bacterial and Yeast

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pCDW115

Plasmid

#70062

Purpose

artificial transcription template "pause ablation"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' A+2G T+6G - repeat cassette - E. coli rpoB - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-4T1 mouse Tankyrase1 5ARC (171–961)

Plasmid

#132638

Purpose

expresses mouse Tankyrase1 5ARC (171–961) in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Protein Sci. 2018 Jun;27(6):1057-1067. d

Insert

Tankyrase1 (171–961)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-P70a-Bottom-T

Plasmid

#87313

Purpose

Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Bottom" transcript

Use

Synthetic Biology

Expression

Bacterial

Promoter

P70a

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pTH07

Plasmid

#70063

Purpose

artificial transcription template "distal pause"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' - repeat cassette - E. coli rpoB with inserted pause - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
36INS-J23100_GB

Plasmid

#78669

Purpose

36INS-J23100_GB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [G:36INS-J23100:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated J23100 Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS-J23100_FB

Plasmid

#78668

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [F:36INS-J23100:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated J23100 Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS-J23100_EB

Plasmid

#78667

Purpose

36INS-J23100_EB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [E:36INS-J23100:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated J23100 Promoter

Use

Synthetic Biology

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pHBFD1

Plasmid

#53565

Purpose

Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

hbfd1 cDNA

Expression

Bacterial

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pRL1058

Plasmid

#70686

Purpose

Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.

Depositor

Peter Wolk

Article

Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

Insert

Transposon Tn5-1058

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pET-28a-CylA

Plasmid

#48198

Purpose

Expresses CylA (fatty acyl-AMP ligase) in E. coli

Depositor

Emily Balskus

Article

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylA

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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T7-AglK-His

Plasmid

#89714

Purpose

Expresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

AglK

Tags

His6 and T7

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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p70rg 1

Plasmid

#17828

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only