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We narrowed to 17,419 results for: She

Showing: 8761 - 8800 of 17419 results

  1. pT7_circRAJ31_C873U

    Plasmid
    #69932
    Purpose
    Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter.
    Depositor
    Alfonso Jaramillo
    Article
    Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do
    Insert
    pT7_circRAJ31_C873U
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    69932-plasmid-map-sequence-id-124224

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  2. pUASp YFP Rac1 CA

    Plasmid
    #52235
    Purpose
    Drosophila expression of YFP-Rac1 constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Rac1 (Rac1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q61L; CA mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. Hs.KRAS4a Q61L

    Plasmid
    #83171
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61L mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Q61L
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83171-plasmid-map-sequence-id-158134

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  4. LMNB2

    Plasmid
    #36896
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    LMNB2 (LMNB2 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains Laminb2 Ig-like domain, amino acid resid…
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pPNT6-eGFP

    Plasmid
    #71303
    Purpose
    This vector can be used for homologous recombination and allows to obtain recombinant neomycin resistant fluorescent cells that can loose fluorescence after Cre expression
    Depositor
    Gian Luca Forni, Marco Musso
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    71303-plasmid-map-sequence-id-130367

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  6. MAT2B

    Plasmid
    #39080
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAT2B (MAT2B Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. Hs.KRAS4b A146T

    Plasmid
    #83150
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), A146T mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    A146T
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83150-plasmid-map-sequence-id-158113

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  8. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid
    #174136
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174136-plasmid-map-sequence-id-341914

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  9. pLPS-hAkt1-gfp

    Plasmid
    #49388
    Purpose
    mammalian expression of human Akt1 fused to EGFP
    Depositor
    Randall McKinnon
    Insert
    Akt1 (AKT1 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    tga to cca at nt 1725 with respect to GenBank ID …
    Promoter
    CMV
    Available since
    Nov. 21, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. DHRS4

    Plasmid
    #39071
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DHRS4 (DHRS4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. Hs.KRAS4a Q61R

    Plasmid
    #83172
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61R mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Q61R
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83172-plasmid-map-sequence-id-158135

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  12. ASPH

    Plasmid
    #39094
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ASPH (ASPH Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pTXB1-CRP/CAP

    Plasmid
    #51563
    Purpose
    Expression of E. coli CRP/CAP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Francisco Malagon, Sankar Adhya
    Insert
    CRP/CAP
    Use
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51563-plasmid-map-sequence-id-78803

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  14. NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1

    Plasmid
    #163319
    Purpose
    Encodes for first COVID protein - NSP1 fused to nano-luciferase
    Depositor
    Dawn Chandler
    Insert
    Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Luciferase
    Tags
    NanoLuc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    163319-plasmid-map-sequence-id-322364

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  15. Myc-gamma3 (in pCDNA3.1)

    Plasmid
    #118041
    Purpose
    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG3 (Gabrg3 Rat)
    Use
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    118041-plasmid-map-sequence-id-228642

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  16. Myc-gamma1 (in pCDNA3.1)

    Plasmid
    #118038
    Purpose
    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG1 (Gabrg1 Rat)
    Use
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118038-plasmid-map-sequence-id-228560

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  17. Myc-gamma3 (in pCAGGS)

    Plasmid
    #118042
    Purpose
    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG3 (Gabrg3 Rat)
    Use
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118042-plasmid-map-sequence-id-228557

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  20. pEF1-puro/hpb1(short)

    Plasmid
    #59973
    Purpose
    integrin b1 short headpiece expression (beta-I, hybrid, PSI domains) expression
    Depositor
    Timothy Springer
    Insert
    human integrin b1 (short) (ITGB1 Human)
    Use
    Tags
    Base coiled-coil and His-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a promoter
    Available since
    Sept. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. Hs.KRAS4b G12A

    Plasmid
    #83142
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12A mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12A
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83142-plasmid-map-sequence-id-158105

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  23. ANKRA2

    Plasmid
    #36904
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ANKRA2 (ANKRA2 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    amino acid residues 148-313 (ANK repeats)
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. MS2_SC2_S_Lambda

    Plasmid
    #175048
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Lambda SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Lambda (Amino Acid 319-869)
    Promoter
    T7
    Available since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175048-plasmid-map-sequence-id-343943

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  26. pJK371

    Plasmid
    #73596
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 3)
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Point mutants in FAD2-3 ORF relative to deposited…
    Promoter
    GAL1 and GAL10
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73596-plasmid-map-sequence-id-139406

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  27. pMT-DREDD-V5-His

    Plasmid
    #63749
    Purpose
    Expresses drosophila DREDD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
    Depositor
    Cyril Gueydan
    Insert
    Death related ced-3/Nedd2-like caspase isoform E (Dredd Fly)
    Use
    Tags
    V5-6XHis
    Expression
    Insect
    Mutation
    Promoter
    pMT
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63749-plasmid-map-sequence-id-108845

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  28. pTRIPZ (M)-HT-Cbx7-ATLm

    Plasmid
    #82522
    Purpose
    Lentivirus vector. Expresses mutant Halotag-Cbx7-ATLm fusion proteins in mammalian cells. Cbx7 ATL mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    mutation of RKR70-72, K75, and KR77-78 to AGA70-7…
    Promoter
    CMV
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82522-plasmid-map-sequence-id-156808

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  30. BMPR2

    Plasmid
    #38972
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BMPR2 (BMPR2 Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  33. pUASp YFP Rho1 DN

    Plasmid
    #52231
    Purpose
    Drosophila expression of YFP-Rho1 dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Rho1 (Rho1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    T19N; DN mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  34. MS2_SC2_S_Alpha

    Plasmid
    #175045
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Alpha SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Alpha (Amino Acid 319-869)
    Promoter
    T7
    Available since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175045-plasmid-map-sequence-id-343939

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  35. MS2_SC2_S_Beta

    Plasmid
    #175046
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Beta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Beta (Amino Acid 319-869)
    Promoter
    T7
    Available since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175046-plasmid-map-sequence-id-343944

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  36. pGEX2TK-YAP-WW1

    Plasmid
    #104305
    Purpose
    bacterial expression of WW domain 1 of YAP; binds PPxY where Y is not phosphorylated
    Depositor
    Marius Sudol
    Insert
    YAP WW1 domain (YAP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    WW domain 1
    Promoter
    tac
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104305-plasmid-map-sequence-id-201233

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  37. pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

    Plasmid
    #159913
    Purpose
    Mutagenesis of Kcnj6
    Depositor
    Larry Zweifel
    Insert
    Kcnj6 (Kcnj6 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159913-plasmid-map-sequence-id-317320

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  39. circRAJ31-Toehold_mutant

    Plasmid
    #69930
    Purpose
    Expresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.
    Depositor
    Alfonso Jaramillo
    Article
    Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do
    Insert
    plTet-circRAJ31_toeholdmutant
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    4 bases mutated in toehold of riboregulator
    Promoter
    PltetO1-2
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    69930-plasmid-map-sequence-id-124217

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  40. 4IA9

    Plasmid
    #51320
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5 (WDR5 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 8761 - 8800 of 17419 results