We narrowed to 12,282 results for: LEA
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Plasmid#186271PurposeExpression vector of His-RanT24N in E.coliDepositorInsertGTP-binding nuclear protein Ran (Ran Mouse)
TagsHis tagged and His-tagged at N terminalExpressionBacterialMutationChanged T24 into NPromoterT7 promoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10333
Plasmid#183955PurposeCAG-dCas12a(WT)-HA-miniVPR-mCherry-T2A-HygroRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNuclease-dead Lb Cas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD832APromoterCAGAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A1.2-c002
Plasmid#173088PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A1 (MACROH2A1 Human)
TagsmCeruleanExpressionBacterialMutationQ162-N372, codon optimizedAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1
Plasmid#176537PurposeNon-protein coding locus components from KraIscB-1DepositorInsertnon-coding locus components from KraIscB-1 locus
ExpressionBacterialAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-OspC
Plasmid#159998PurposeExpresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cellsDepositorInsertSpyTag003-OspC (20-209) C129S
TagsHis6-tag and Thrombin cleavage siteExpressionBacterialMutationC129S mutation improves expression level.PromoterT7Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG
Plasmid#155131PurposeExpresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
TagsHisExpressionBacterialMutationinsert codes for GlpG residues 87-276PromoterT7Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pASPIre1
Plasmid#154842PurposeDerivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sitesDepositorInsertBxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
ExpressionBacterialPromoterPrhaAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-Myb
Plasmid#139826Purposedoxycycline-inducible expression of mouse Myb in mammalian cellsDepositorInsertMyb (Myb Mouse)
UseLentiviralExpressionMammalianMutationP94H and Q619R- Please see depositor comment belo…PromoterTRE-mCMVAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE2-TetOminiCMV-Klf4
Plasmid#136613PurposeDox-inducible lentiviral vector expressing mouse Klf4DepositorAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Emerin
Plasmid#120246PurposeExpresses V5-tagged Emerin in lentiviral vectorDepositorAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rag2 T490A
Plasmid#115650Purposeexpression of full length Rag2 with T490A mutationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRag2 (Rag2 Mouse)
Tags8XHis-MBP-PreScissionExpressionMammalianMutationT490AAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP
Plasmid#112631PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral RGDSP integrin-binding sequencePromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB416
Plasmid#68681PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsNuclear localization signal-tagged tdTomato (tdTo…ExpressionPlantAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
5C7M (ITCH)
Plasmid#69966PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertITCH:SDC094-D06:C29524 (ITCH Human)
TagsHis and TEV cleavage siteExpressionBacterialPromoterT7-lacOAvailable sinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdEos-Nucleus-7
Plasmid#57650PurposeLocalization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSV40 T-Antigen Nuclear Localization Signal (NLS)
TagstdEos-NLSExpressionMammalianPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBT296_pBS-U-attP-FRT5-pSV40-Neo-pA-FRT5
Plasmid#52537PurposeExpression of FRT5-flanked Neo; homologous recombination targeting vector cloningDepositorInsertsPhiC31o attP (full length)
Neo
ExpressionMammalianAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GSSGSS NusA TEV LIC cloning vector (2NTL)
Plasmid#55214DepositorTypeEmpty backboneTags6xHis, GSSGSS linker, NusA, and TEV cleavage site…ExpressionBacterialPromoterT7Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCY 3010-07
Plasmid#36212DepositorInsertStreptoalloteichus hindustanus bleomycin (Sh ble)
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-Cerulean-ZeocinAvailable sinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1418
Plasmid#29214PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPle112 (HCRT Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSAvailable sinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTH278-GST-TEV-SUP45
Plasmid#29743DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
TagsGSTExpressionBacterialMutationwild typeAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only