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pENTR/pSM2(CMV) GFP (w513-1)

Plasmid

#19170

Purpose

Gateway entry vector for miRNA expression under CMV promoter and GFP reporter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and RNAi

Tags

GFP

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pEYFP-C1-p85beta

Plasmid

#1408

Depositor

Lewis Cantley

Insert

PI3K regulatory subunit p85beta (Pik3r2 Mouse)

Tags

eYFP

Expression

Mammalian

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-RIPK3

Plasmid

#78804

Purpose

To overexpress RIPK3 in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-mCherry-Syk

Plasmid

#50045

Purpose

Expression of mCherry-Syk in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

Spleen tyrosine kinase (Syk) (Syk Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-fNPY-GFP

Plasmid

#22912

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu neuropeptide Y promoter driving GFP

Use

AAV

Expression

Mammalian

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pLV-STAT2

Plasmid

#71451

Purpose

3rd generation lentiviral expression of STAT2

Depositor

George Stark

Article

Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

Insert

STAT2 (STAT2 Human)

Use

Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCS-H2B-EGFP

Plasmid

#53744

Purpose

Contains human Histone H2B fused to EGFP in the pCS2+ expression vector

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

EGFP

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

June 27, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-ER Y537S

Plasmid

#49499

Purpose

Expresses HA-tagged ER Y537S mutant in mammalian cells

Depositor

Sarat Chandarlapaty

Article

Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

Insert

ESR1 (ESR1 Human)

Tags

HA

Expression

Mammalian

Mutation

Tyrosine 537 to Serine

Promoter

CMV

Available Since

Dec. 12, 2013

Availability

Academic Institutions and Nonprofits only

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Flag Depdc5 pRK5

Plasmid

#46340

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV4-NIK-HA

Plasmid

#27554

Depositor

Shao-Cong Sun

Article

Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

Insert

NIK (MAP3K14 Human)

Tags

HA

Expression

Mammalian

Mutation

NIK starts at amino acid 3; C52R and R139H mutati…

Promoter

CMV

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
Mito-mEosEM

Plasmid

#132706

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mitochondria targeting sequence

Tags

mEosEM

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Nano-lantern/pcDNA3

Plasmid

#51970

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XMyc-EGFP-PEX26

Plasmid

#139059

Purpose

Myc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Article

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XMyc-EGFP-PEX26

Use

Lentiviral

Tags

Myc

Promoter

UBC

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK-14-3-3 eta GST

Plasmid

#13277

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 eta (YWHAH Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO V5 HSPA8

Plasmid

#19514

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 8 (HSPA8 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pCS2 GFP-GSK3-MAPK

Plasmid

#29689

Purpose

GFP reporter protein fused to three GSK3 phosphorylation sites

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

GFP glycogen synthase kinase 3 recognition site primed by MAPK

Tags

Flag and Flag-EGFP

Expression

Mammalian

Mutation

recognition sites only, and E3 ligase site

Promoter

SP6

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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HA-EglN3-pcDNA3

Plasmid

#18960

Depositor

William Kaelin

Article

Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

Insert

Egg laying Nine - 3 (EGLN3 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-hYAP2

Plasmid

#17844

Depositor

Marius Sudol

Article

Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

Insert

YAP (YAP1 Human)

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-CWB-yellow pre-eGRASP(p32)

Plasmid

#111580

Purpose

An AAV vector that expresses yellow pre-eGRASP(p32) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

yellow pre-eGRASP(p32)

Use

AAV

Promoter

CaMKIIa

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 tdAPX W41F

Plasmid

#40863

Purpose

Tandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

tandem dimer pea APX W41F

Tags

Flag

Expression

Mammalian

Mutation

W41F mutation

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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