We narrowed to 10,234 results for: UTY

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SPLINTR GFP v1 backbone

Plasmid

#179777

Purpose

SPLINTR backbone vector

Depositor

Mark Dawson

Article

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEF Doc2beta WT GFP

Plasmid

#128820

Purpose

To visualize Doc2beta WT in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Tags

EGFP

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2

Plasmid

#113945

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-839

Promoter

CMV

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX

Plasmid

#188991

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

F163L, K249R, G328S, G381V, G429D, D480G

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLX302-JSP1-V5 puro

Plasmid

#87773

Purpose

Expresses V5 tagged human JSP1 (DUSP22)

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

JSP1 (DUSP22 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMCL-HA-MAPKK1-G1C [delta(44-51)/S218E/S222D]

Plasmid

#40807

Depositor

Natalie Ahn

Article

Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

Insert

MAP2K1 (MAP2K1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta(44-51), S218E, S222D

Promoter

CMV

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pLCHKO

Plasmid

#155048

Purpose

Lentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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p35u4

Plasmid

#174786

Purpose

Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system

Depositor

Katherine Berry

Article

Wang et al RNA. 2021 Apr;27(4):513-526. doi: 10.126

Insert

MS2 coat protein

Tags

λCI

Expression

Bacterial

Mutation

V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…

Promoter

Synthetic Constitutive Promoter

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB rhoA QL

Plasmid

#61632

Purpose

mammalian expression of rhoA constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

rhoA (RHOA Human)

Expression

Mammalian

Mutation

Q63L, constitutively active

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
SaCas9 TadCBEa

Plasmid

#193844

Purpose

Express TadCBEa (with SaCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

SaCas9 TadCBEa

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB cdc42 QL

Plasmid

#61636

Purpose

mammalian expression of cdc42 constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

Cdc42 (CDC42 Human)

Expression

Mammalian

Mutation

Q61L, constitutively active

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pHM123DEnv

Plasmid

#137668

Purpose

Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

Depositor

Anjali Joshi

Article

Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

Insert

HIV-1 Envelope Glycoprotein

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn-HpaBC

Plasmid

#100949

Purpose

Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Inserts

RgTALsyn
HpaB
HpaC

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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Dendra2-Vinculin-N-21

Plasmid

#57749

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

NM_003373.3 (VCL Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pEDF-PhdRS

Plasmid

#127445

Purpose

Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage

Depositor

Wenshe Liu

Article

Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:

Insert

Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair

Expression

Bacterial

Mutation

N346A-C348A mutations on PylRS

Promoter

araBAD

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-mTDGa.0

Plasmid

#81050

Purpose

bacterial expression of murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP

Plasmid

#192273

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloning

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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WTTEM1_pSTC0-zeo

Plasmid

#202351

Purpose

Control WT TEM-1 (wild type TEM-1 beta lactamase) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

TEM-1

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

Plasmid

#105039

Purpose

Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tsc2 (Tsc2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W

Plasmid

#105034

Purpose

Lentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Ptpn23 (Ptpn23 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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