We narrowed to 31,602 results for: REP

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pBS-KS-attB2-SA(0)-T2A-Gal4DBD-Hsp70

Plasmid

#62902

Purpose

Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4DBD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

Plasmid

#168107

Purpose

PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry

Depositor

Erich Jarvis

Article

Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

Insert

Cas9-P2A-mCherry

Use

CRISPR and Cre/Lox

Tags

HA

Promoter

CAG

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pTet-O-NEUROG1-T2A-PuroR

Plasmid

#162339

Purpose

Lentiviral expression of NEUROG1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

NEUROG1 (NEUROG1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGria2

Plasmid

#124854

Purpose

Mutagenesis of Gria2

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria2 (Gria2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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ACTIN-SOX2-PGK Cre

Plasmid

#59019

Purpose

Bicistronic Lentivirus expressing sox2 and Cre

Depositor

Trudy Oliver

Article

Mukhopadhyay et al Cell Rep. 2014 Jul 10;8(1):40-49. doi: 1

Insert

Sox2 (Sox2 Mouse)

Use

Lentiviral

Promoter

B-actin

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
CRY2clust-mCherry-RANK

Plasmid

#208612

Purpose

Mammalian expression of CRY2clust-mCherry-RANK (Opto-RANKc)

Depositor

Tomohiro Ishii, Takao Nakata

Article

Takada et al Sci Rep. 2024 Jan 19;14(1):1749. doi: 10

Insert

CRY2clust-mCherry-RANK (Cry2 Mouse, Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET17b-yebST

Plasmid

#175804

Purpose

Complementation of yebST knockout in E. coli K-12

Depositor

Gira Bhabha, Damian Ekiert, Ian Henderson

Article

Isom et al Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.

Insert

yebST

Tags

None

Expression

Bacterial

Mutation

silent mutations to introduce restriction sites

Promoter

T7

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

Plasmid

#115789

Purpose

Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

NDP (NDP Human)

Tags

alkaline phosphatase and flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-Sestrin3

Plasmid

#72592

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN3 (SESN3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only
pBS-KS-attB2-SA(1)-T2A-LexA::QFAD-Hsp70

Plasmid

#62948

Purpose

Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-LexA::QFAD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pPB12A

Plasmid

#210035

Purpose

Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas12a
rtta-T2A-puro

Use

CRISPR

Tags

nucleoplasmin NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)

Plasmid

#197893

Purpose

Can be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

mCherry

Use

AAV

Mutation

N/A

Promoter

EF1a

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

Plasmid

#168106

Purpose

PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning

Depositor

Erich Jarvis

Article

Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

Insert

mCherry-KASH

Use

CRISPR and Cre/Lox

Promoter

CAG

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGrin2a

Plasmid

#124850

Purpose

Mutagenesis of Grin2a

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2a (Grin2a Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA

Plasmid

#154775

Purpose

plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Expression

Mammalian

Mutation

150TAA in GFP reporter, UUA anticodon and G10A, …

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S32-KD

Plasmid

#140311

Purpose

Expresses Flag-tagged human Src-S32-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

Contains only the SH3, SH2, and KD regions

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-KD

Plasmid

#140297

Purpose

Expresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

contains only the SH4 and KD regions

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6

Plasmid

#124860

Purpose

Mutagenesis of Slc17a6

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a6 (Slc17a6 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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PARP1-c009

Plasmid

#216872

Purpose

Bacterial expression vector for E. coli producing PARP1 catalytic protein (without Regulatory domain); with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

Insert

PARP1 catalytic domain (no Regulatory Domain) (PARP1 Human)

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0 miR-27a-3p

Plasmid

#82438

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

hsa-miR-27a-3p (MIR27A Human)

Mutation

Three copies hsa-miR-27a-3p Complementary region …

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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