We narrowed to 10,034 results for: Pol
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Plasmid#137104PurposeiFLinkC Linker cloningDepositorInsertGGA(EAAAK)2AGG
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK187
Plasmid#156423PurposeInsect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein
TagssfGFP-HisExpressionInsectPromoterPolyhedrinAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP
Plasmid#112631PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral RGDSP integrin-binding sequencePromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
MX01
Plasmid#158624PurposeGATEWAY and BacMam compatible mammalian expression vector with eGFP expression reporter (eGFP is expressed by same promotor but as a separate polypeptide chain).DepositorTypeEmpty backboneUseGateway destination vector ; bacmam acceptor vect…TagsP2AExpressionMammalianPromoterCMV-IEAvailable SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDB036d
Plasmid#99216PurposeAccessory plasmid for PACE of MjTyrRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
ExpressionBacterialMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK, and PproAAvailable SinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-amidase
Plasmid#128135PurposeThe cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertamidase
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-nCas9n-A71
Plasmid#125644Purposemicro-injection of poly-adenylated nCas9n RNADepositorInsertnCas9n
UseRna in vitro transcription vectorPromoterT3Available SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_BE3_IVT
Plasmid#177015PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymeraseDepositorInsertBE3
UseCRISPR; Vector for in-vitro transcriptionPromoterT3 promoterAvailable SinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB036a
Plasmid#99215PurposeAccessory plasmid for PACE of MjTyrRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
ExpressionBacterialMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK and PproDAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS4
Plasmid#55751PurposeProduces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
ExpressionBacterialMutationResidues 1-59 of KanRPromoterT7-lacAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a
Plasmid#216385PurposeBaculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)DepositorInsertNorrin (NDP Human)
UseBaculovirusTagsGP64 signal sequence-MBP-3C and Rho1D4ExpressionInsectMutationC93A/C95A/C131APromoterPolyhedrinAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pV2A-T
Plasmid#153022PurposeSingle-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoterDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOCC114
Plasmid#118904Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase siteDepositorInsertNcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with TEV protease, two glycinesExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
3924-55
Plasmid#176499PurposepTRE-EGFP-T2A-TFEB(S3E)-polyADepositorInsertEGFP-T2A-TFEB(human; full-length; S3E mutation)-
ExpressionMammalianPromoterTREAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
3923-51
Plasmid#176498PurposepTRE-EGFP-T2A-TFEB(S3A)-polyADepositorInsertEGFP-T2A-TFEB(human; full-length; S3A mutation)
ExpressionMammalianPromoterTREAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092686
Plasmid#153895PurposeBacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTRDepositorInsertfragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionBacterialMutationNearly native encoding;inactivating mutations in …PromoterPromoter | t7 consensus from NEB, G is +1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p3PA-Puro-iTk
Plasmid#109067Purposeinserts three polyA sites to end transcription of a targetted gene and a puromycin cassette for selectionDepositorTypeEmpty backboneExpressionMammalianPromoterEFSAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 P186C
Plasmid#66173PurposeExpresses the P186C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
TagsHis6 tagExpressionBacterialMutationP186CPromoterT7 promoterAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRJPaph-eBFP2
Plasmid#67030PurposePlasmid for Paph-eBFP2 integration downstream of B. diazoefficiens (B. japonicum) scoIDepositorInsertB. diazoefficiens DNA from scoI downstream region (comprising blr1132')
UseConjugative bacterial vectorAvailable SinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT eGFP-RVFV NSs HhRz
Plasmid#91801PurposeExpresses a non-polyadenylated, translatable EGFP mRNA in DrosophilaDepositorInsertEGFP
ExpressionInsectPromoterMetallothionein Promoter (pMT)Available SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 WT
Plasmid#66139PurposeExpresses the wild type of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRUSH
Plasmid#54479PurposeMouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to drive short RNAs, pgkneo, cassette flanked by loxP sitesDepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and RNAiTagsSA-EGFP-polyA, U6 promoter, loxP, and pgk-neoExpressionMammalianPromoterU6Available SinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
FAK
Plasmid#186141PurposeFor expresseion of recombinant FAK using baccolovirus in SF9 cellsDepositorAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WF
Plasmid#108337PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PQBP1
Plasmid#16174DepositorInsertPolyglutamine binding protein 1 (PQBP1 Human)
ExpressionMammalianAvailable SinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
C239-E03: attB2r-IRES-ffLuc2-pA-attB3
Plasmid#162919PurposeGateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2 reporter using an internal ribosome entry sequence (IRES)DepositorInsertfirefly luciferase with upstream IRES sequence
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS1
Plasmid#55749PurposeProduces aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
ExpressionBacterialPromoterT7-lacAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
MTK4b_001
Plasmid#123840PurposeEncodes Bovine Growth Hormone polyadenylation signal as a Type 4b part to be used in the MTK systemDepositorInsertBgh pA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBac(3xP3-gTc’v; pUb:lox-mYFP-lox-H2BmCherry)
Plasmid#119064PurposeCre/lox flip-out construct for marking cell clones in Tribolium castaneumDepositorInsertspBacL
pUb regulatory sequence
loxN
mYFP
loxN
H2B-mcherry
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR
ExpressionInsectAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z globin NB Probe
Plasmid#120390PurposeAllows in vitro SP6 Polymerase driven transcription of a beta globin northern blot probeDepositorInsertbeta globin partial 3' UTR
UseUnspecifiedAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BR
Plasmid#108336PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WF
Plasmid#108333PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BF
Plasmid#108334PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BF
Plasmid#108338PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WR
Plasmid#108339PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BR
Plasmid#108340PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 L171C
Plasmid#66177PurposeExpresses the L171C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
TagsHis6 tagExpressionBacterialMutationL171CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 G108C
Plasmid#66140PurposeExpresses the G108C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
TagsHis6 tagExpressionBacterialMutationG108CPromoterT7 promoterAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 WT
Plasmid#66179PurposeExpresses the wild type of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 WT
Plasmid#66175PurposeExpresses the wild type of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only