We narrowed to 11,193 results for: AGA

1. pCX-SponGee-Kras

   Plasmid

   #134777

   Purpose

   mammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

   Insert

   SponGee-Kras
   

   Tags

   FLAG and mRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTXB1-H2B(1-114)_wt

   Plasmid

   #118252

   Purpose

   Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa

   Depositor

   Cynthia Wolberger

   Article

   Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

   Insert

   xlH2B(1-114)
   

   Tags

   Mxe-GyrA intein fused to chitin binding domain

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF6 VPS51-V5-6His

   Plasmid

   #175502

   Purpose

   V5 tagged VPS51 is expressed in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Perez-Victoria et al Mol Biol Cell. 2010 Oct 1;21(19):3386-95

   Insert

   VPS51 (VPS51 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   A661V- please see depositor comments

   Available Since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST- IP2.0(codon for C.elegans)

   Plasmid

   #109216

   Purpose

   IP2.0 for C. elegans (Codon optimization and introns).

   Depositor

   Takeshi Ishihara

   Article

   Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

   Insert

   IP2.0 (Inverse Pericam 2.0)
   

   Tags

   His-tag

   Expression

   Worm

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-HA-BLMP-1-pcDNA3.1-

   Plasmid

   #52512

   Purpose

   expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   Blmp-1 (blmp-1 Nematode)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   N75S mutation compared GenBank reference sequence…

   Promoter

   CMV

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT15415-BEAR-GFP-target-mCherry

   Plasmid

   #162995

   Purpose

   plasmid expressing an sgRNA targeting the BEAR-GFP plasmid along with an mCherry marker

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   sgRNA targeting the BEAR-GFP plasmid
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. shPBRM1 #122-Tet-pLKO-puro

   Plasmid

   #107401

   Purpose

   Lentiviral expression of shRNA targeting PBRM

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   shRNA targeting PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tol2-LyzC-GFP-U6ac-ctrl-guides

   Plasmid

   #168240

   Purpose

   "neutrophil specific GFP with ubiquitous ctrl sgRNAs"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   control sgRNAs
   

   Use

   CRISPR

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PBLO CasX-gRNA

   Plasmid

   #126419

   Purpose

   E. coli vector for CasX gRNA --- Used for In Vitro Transcription

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   casX gRNA
   

   Use

   CRISPR; In vitro transcription

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. cfmTurquoise-N

    Plasmid

    #139284

    Purpose

    Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.

    Depositor

    Ikuo Wada

    Article

    Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d

    Insert

    cfmTurquoise
    

    Tags

    Multi-cloning sites

    Expression

    Mammalian

    Mutation

    Cys48Ser and Cys70/Met

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmyc-GFP-TNRC6A-NES-mut

    Plasmid

    #42001

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    F951A, F955A, I958A, F960A

    Promoter

    CMV

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV asyn S87A

    Plasmid

    #36056

    Depositor

    Hilal Lashuel

    Article

    Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

    Insert

    alpha-synuclein S87A (SNCA Human)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S87A

    Promoter

    CMV

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3HA-ErbB4CTF

    Plasmid

    #17803

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    ErbB4 (ERBB4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Carboxy-terminal fragment, amino acids 676-1292

    Available Since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

14. GFP-SP110C

    Plasmid

    #65393

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SP110C
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pPGP_scFv

    Plasmid

    #183732

    Purpose

    Expresses anti-lysozyme scFv antibody

    Depositor

    Jun Ishii

    Article

    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

    Insert

    Anti-lysozyme scFv
    

    Tags

    His6-tag

    Expression

    Yeast

    Promoter

    GAP promoter

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PLK1 G5.1 gRNA

    Plasmid

    #90834

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PLK1 (Guide Designation G5.1)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTorPE-GEM-GECO1

    Plasmid

    #32463

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEM-GECO1
    

    Use

    Lab constructed

    Tags

    6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…

    Expression

    Bacterial

    Mutation

    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV-PIG-N3FLAG-TAF10

    Plasmid

    #105990

    Purpose

    retrovirally express mouse TAF10 with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    FLAG tagged TAF10 (Taf10 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Promoter

    MSCV-LTR

    Available Since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1-blast shGAC

    Plasmid

    #110410

    Purpose

    shGAC (Target CCTCTGTTCTGTCAGAGTT), silence glutaminase isoform GAC, blasticidin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 (RNA Pol III)

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-CAG-DIO-erHRP(N175S mutant)

    Plasmid

    #79906

    Purpose

    Adeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)

    Depositor

    Joshua Sanes

    Article

    Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

    Insert

    erHRP(N175S mutant)
    

    Use

    AAV and Cre/Lox

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    N175S mutation

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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