We narrowed to 11,021 results for: ENA;

1. pCI Cas9 H2B-RFP

   Plasmid

   #92397

   Purpose

   CAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRES

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF-ABEmax

   Plasmid

   #164415

   Purpose

   EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   ABEmax
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. G1333 DddAtox-N–SaKKH-Cas9(D10A)

   Plasmid

   #157837

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1333 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. ND4 right TALE–G1397 DddAtox C

   Plasmid

   #158097

   Purpose

   expresses the right-side ND4 TALE–DddAtox half in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA tox-C–1xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. ND4 left TALE–G1397 DddAtox N

   Plasmid

   #158094

   Purpose

   expresses the right-side ND4 TALE–DddAtox half in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   SOD2 MTS–3xHA–ND4 left TALE–G1397 DddAtox-N–1xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMJ923

   Plasmid

   #78313

   Purpose

   Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli

   Depositor

   Martin Jinek

   Article

   Burger et al Development. 2016 Apr 29. pii: dev.13480

   Insert

   SpCas9
   

   Use

   Tags

   HA-2xNLS-mCherry-NLS and His6-MBP-TEV

   Expression

   Bacterial

   Mutation

   human codon-optimized gene sequence

   Promoter

   T7

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

   Plasmid

   #105043

   Purpose

   Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Stk11 (Stk11 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_B2101

   Plasmid

   #91060

   Purpose

   Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   35S

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET-pr-mEos3.2 (mEosFP3.2-A69T)

   Plasmid

   #99224

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEos3.2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A69T for primed conversion applications

   Promoter

   T7

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSyn-ChRger1-TS-EYFP

    Plasmid

    #127243

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger1-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127321

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFD5-U6-3-t-attP40

    Plasmid

    #133561

    Purpose

    gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    attP40 region guide RNAs
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRDB_181

    Plasmid

    #216102

    Purpose

    CRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-RBP-LOV2

    Plasmid

    #185792

    Purpose

    Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-LOV2
    

    Use

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiCRISPR v2-sgAMPK alpha1 clone1

    Plasmid

    #162119

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. LacI-pLacI-pII pm

    Plasmid

    #218099

    Purpose

    Phagemid that expresses LacI, and pII under the Lac promoter

    Depositor

    Kevin Esvelt

    Insert

    pII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AA179

    Plasmid

    #215939

    Purpose

    Fragmid fragment: (guide cassette) short sequence with no known function

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Filler
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mCherry-NES-SspB-MCS-P2A-iLIDcaax

    Plasmid

    #173869

    Purpose

    Mammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-MCS-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Dead Streptavidin-SpyCatcher

    Plasmid

    #59547

    Purpose

    pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

    Insert

    Dead-dloop-Streptavidin-SpyCatcher
    

    Use

    Tags

    N-terminally shortened SpyCatcher

    Expression

    Bacterial

    Mutation

    PolyAsp in L3/4 loop for ion-exchange

    Promoter

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

20. EGFP-Ahi1

    Plasmid

    #30494

    Depositor

    Joseph Gleeson

    Article

    Lancaster et al Nat Med. 2009 Sep . 15(9):1046-54.

    Insert

    Ahi1 (AHI1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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