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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,798 results for: IRES

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Showing: 4441 - 4460 of 4798 results

  1. myc-hIFITM3-S61A,N64A,T65A

    Plasmid
    #104362
    Purpose
    Expresses human IFITM3-S61A,N64A,T65A with an N-terminal myc tag in mammalian cells.
    Depositor
    Jacob Yount
    Publication
    Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IFITM3 (IFITM3 Human)
    Tags
    myc
    Expression
    Mammalian
    Mutation
    mutated the following amino acids: S61A,N64A,T65A
    Promoter
    CMV
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    104362-plasmid-map-sequence-id-199999

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  3. ST-SBP-mCherry_puromycin

    Plasmid
    #65271
    Purpose
    ST reporter only ( no hook) (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    truncated sialyltransferase (ST6GAL1 Human)
    Tags
    Streptavidin Binding Protein (SBP) and mCherry
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 43 of ST
    Promoter
    CMV
    Available since
    June 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    65271-plasmid-map-sequence-id-110479

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  6. pcDNA3.1-mPCSK3 S368A

    Plasmid
    #122680
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)
    Depositor
    Liming Pei
    Publication
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Expression
    Mammalian
    Mutation
    changed Serine 368 to Alanine
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122680-plasmid-map-sequence-id-244743

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  7. pcDNA3.1-mPCSK3 H194L

    Plasmid
    #122689
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)
    Depositor
    Liming Pei
    Publication
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Expression
    Mammalian
    Mutation
    Changed Histidine 194 to Leucine
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122689-plasmid-map-sequence-id-244742

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  10. pENTR1A mKate2-hu_ncOGT 5N5A

    Plasmid
    #154285
    Purpose
    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)
    Depositor
    Suzanne Walker
    Publication
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Gateway Cloning
    Tags
    2x FLAG and mKate2
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations (HCF-…
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154285-plasmid-map-sequence-id-304861

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  12. pDEST-Myc-FBW7 WD40 (All deleted)

    Plasmid
    #197455
    Purpose
    The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 All are deleted. Used for mammalian overexpression and detection by western blotting.
    Depositor
    Omar Khan
    Publication
    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:
    Insert
    FBW7 (FBXW7 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FBW& dimerization domain deleted.
    Promoter
    CMV
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    197455-plasmid-map-sequence-id-394905

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  16. pTT5-hSDC1-CS1

    Plasmid
    #52365
    Purpose
    expresses human Syndecan-1 point mutant at S184A in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Publication
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SDC1 (SDC1 Human)
    Expression
    Mammalian
    Mutation
    R95Q and S184A (according to numbering in Fig. 2A…
    Promoter
    CMV
    Available since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)

    Plasmid
    #171101
    Purpose
    CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.
    Depositor
    Joseph Brzezinski
    Publication
    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9 (Olig2 S. pyogenes, Mouse)
    Use
    CRISPR
    Promoter
    Ef1a
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    171101-plasmid-map-sequence-id-336130

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  20. pTT5-hSDC1-N20

    Plasmid
    #52355
    Purpose
    expresses human syndecan 1 'del 1- 20' truncation mutant in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Publication
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SDC1 (SDC1 Human)
    Expression
    Mammalian
    Mutation
    Deletion of SDC1 aa2-21 (according to numbering i…
    Promoter
    CMV
    Available since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
Showing: 4441 - 4460 of 4798 results