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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,812 results for: cloning

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Showing: 4441 - 4460 of 63812 results

  4. pCMV-CD63-L7Ae

    Plasmid
    #180503
    Purpose
    Human CD63-L7Ae fusion protein
    Depositor
    Masaharu Somiya
    Publication
    Somiya et al bioRxiv 2022.01.25.477620
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human CD63-L7Ae fusion protein
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    180503-plasmid-map-sequence-id-357065

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  5. pX458-Dual-Guide-Donor-ShuttleOnly

    Plasmid
    #171098
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Publication
    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. GtoC mu…
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    171098-plasmid-map-sequence-id-336112

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  7. PGK plasmid

    Plasmid
    #169744
    Purpose
    Unregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.
    Depositor
    Francois St-Pierre
    Publication
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    PGK promoter
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169744-plasmid-map-sequence-id-334452

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  8. SpyCas9 PE2*

    Plasmid
    #169850
    Purpose
    Mammalian Expression, SpyCas9 prime editor, optimized NLS
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9 PE*
    Tags
    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    June 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169850-plasmid-map-sequence-id-332905

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  9. pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX

    Plasmid
    #167583
    Purpose
    Express the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cells
    Depositor
    Yulong Li
    Publication
    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    167583-plasmid-map-sequence-id-330893

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  11. pUCM-CLYBL-NGN2-BRN3A

    Plasmid
    #165597
    Purpose
    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.
    Depositor
    Carsten Bönnemann
    Publication
    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Promoter
    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…
    Available since
    March 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165597-plasmid-map-sequence-id-326012

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  13. pExp-GB1

    Plasmid
    #112565
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-GB1 and Strep tag
    Expression
    Bacterial
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112565-plasmid-map-sequence-id-217228

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  14. Yeast Secrete and Detect

    Plasmid kit
    #1000000166
    Purpose
    Assembly of transcription units for protein secretion in the yeasts Saccharomyces cerevisiae and Pichia pastoris.
    Depositor
    Sylvestre Marillonnet
    Application
    Cloning and Synthetic Biology
    Vector type
    Yeast Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  19. pMTL3

    Plasmid
    #127966
    Purpose
    constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-CAG-KRAB-dCas9-BFP-KRAB
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    127966-plasmid-map-sequence-id-249160

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  20. pMTL5

    Plasmid
    #127967
    Purpose
    DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    127967-plasmid-map-sequence-id-249184

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Showing: 4441 - 4460 of 63812 results