We narrowed to 59,444 results for: cloning

1. p663-UBC-miniTurbo-V5-TLK1_IDG-K

   Plasmid

   #170588

   Purpose

   Gateway destination clone of TLK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-TLK1 (TLK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBlueScript NCX1.1

   Plasmid

   #66799

   Depositor

   Michela Ottolia, Kenneth Philipson

   Article

   Nicoll et al Science. 1990 Oct 26;250(4980):562-5.

   Insert

   NCX1.1
   

   Use

   T3 transcription of gene

   Mutation

   there are 3 silent mutations within the open read…

   Promoter

   T3

   Available Since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. p663-UBC-miniTurbo-V5-CSNK1A1L_IDG-K

   Plasmid

   #170591

   Purpose

   Gateway destination clone of CSNK1A1L (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CSNK1A1L (CSNK1A1L Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p663-UBC-miniTurbo-V5-VRK2_IDG-K

   Plasmid

   #158196

   Purpose

   Gateway destination clone of VRK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-VRK2 (VRK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEYFPpA-ACN

   Plasmid

   #20344

   Depositor

   Mario Capecchi

   Article

   Wu et al Nat Protoc. 2008 . 3(6):1056-76.

   Insert

   EYFPpA-ACN
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Available Since

   March 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-L3-HCN1miR-EGFP-L2

   Plasmid

   #32585

   Depositor

   Matthew Nolan

   Article

   White et al Front Mol Neurosci. 2011;4:8. Epub 2011

   Insert

   Intronic HCN1miRNA and EGFP
   

   Use

   Gateway cloning vector

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS Coupler 1

   Plasmid

   #185824

   Purpose

   Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

   Depositor

   Atsushi Miyawaki

   Article

   Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

   Insert

   (GlyGlyGlyGlySer)x3 linker
   

   Use

   Cloning vector

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFH80

   Plasmid

   #128191

   Purpose

   Level1 ZmUBIp::xCas9 3.7:NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::xCas9 3.7:NOSt
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pECFPpA-ACN

   Plasmid

   #20301

   Depositor

   Mario Capecchi

   Article

   Wu et al Nat Protoc. 2008 . 3(6):1056-76.

   Insert

   ECFPpA-ACN
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Available Since

   March 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. R5 MCS

    Plasmid

    #51732

    Purpose

    Dicistronic vector with cloning site

    Depositor

    Vincent Mauro

    Article

    Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

    Insert

    MCS
    

    Promoter

    SV40

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. CHKB (3FEG)

    Plasmid

    #25302

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CHKB:r35-s395 (CHKB Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids R35-S395

    Available Since

    Aug. 16, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

12. HOXD8 (human,partial) HIS-tag pET

    Plasmid

    #8569

    Depositor

    Corey Largman

    Insert

    HOXD8 (HOXD8 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Partial protein containing 4 AA N-terminal to HD …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMK-mEmerald

    Plasmid

    #123556

    Purpose

    MoChlo kit

    Depositor

    Scott Lenaghan, C. Neal Stewart

    Article

    Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

    Insert

    mEmerald
    

    Use

    Synthetic Biology

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p663-UBC-miniTurbo-V5-TESK2_IDG-K

    Plasmid

    #170615

    Purpose

    Gateway destination clone of TESK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-TESK2 (TESK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    July 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. NMT1 (3IU2)

    Plasmid

    #25272

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NMT1:R115-Q496 (NMT1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids R115-Q496

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pFH76

    Plasmid

    #125815

    Purpose

    Level0 ScCas9, wheat codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ScCas9, wheat codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-L5-EGFP-L2

    Plasmid

    #32593

    Depositor

    Matthew Nolan

    Article

    White et al Front Mol Neurosci. 2011;4:8. Epub 2011

    Insert

    EGFP (eGFP )
    

    Use

    Gateway cloning vector

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. p667-UBC-SBK2-V5-miniTurbo_IDG-K

    Plasmid

    #189899

    Purpose

    Gateway destination clone of SBK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    SBK2 (SBK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVYD

    Plasmid

    #61302

    Purpose

    Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to SYFP2

    Depositor

    Julia Vorholt

    Article

    Kaczmarczyk et al Sci Rep. 2014 Sep 29;4:6453. doi: 10.103

    Type

    Empty backbone

    Tags

    SYFP2

    Expression

    Bacterial

    Promoter

    PV10

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. p663-UBC-miniTurbo-V5-PHKG2_IDG-K

    Plasmid

    #158186

    Purpose

    Gateway destination clone of PHKG2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-PHKG2 (PHKG2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge