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W118-1_Flag-hIRAK1

Plasmid

#180405

Purpose

lentiviral transduction of human IRAK1 (with an N-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

IRAK1 (IRAK1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Klf4

Plasmid

#172870

Purpose

Mammalian expression vector with myc-tagged mouse Klf4 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Klf4 (Klf4 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS1991

Plasmid

#49122

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-iCre-WPRE

Use

AAV

Expression

Mammalian

Promoter

FEV

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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CXCR6-DuET

Plasmid

#213223

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CXCR6 (CXCR6 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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SaCas9 GFP V3

Plasmid

#226962

Purpose

CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B

Plasmid

#180380

Purpose

lentiviral transduction of human ZBTB7B gene

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B (ZBTB7B Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Grhl3

Plasmid

#172869

Purpose

Mammalian expression vector with myc-tagged mouse Grhl3 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Grhl3 (Grhl3 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLCKOv3

Plasmid

#209046

Purpose

Lentiviral backbone for expressing U6 driven SpCas9 sgRNAs with BveI cloning sites, SpCas9 tracrRNAv1 (Dang et al., 2015; PMID: 26671237) and puromycin selection marker

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB8

Plasmid

#63109

Purpose

Expression of HSPB8 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb8 (HSPB8 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB5

Plasmid

#63106

Purpose

Expression of HSPB5 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb5 (CRYAB Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 S D614

Plasmid

#158074

Purpose

Expresses SARS-CoV-2 spike protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pPPI4-SNAP-CTLA-4-His6

Plasmid

#223584

Purpose

Protein purification of human CTLA4 with SNAP and 6xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CTLA4 (CTLA4 Human)

Tags

His6 and SNAP

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DEST TNRC6A

Plasmid

#19883

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6A (TNRC6A Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pmCTIP2-N174

Plasmid

#60858

Purpose

Expresses CTIP2 under the EF1a promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

CTIP2 isoform b (Bcl11b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-MDA5-S88D

Plasmid

#174226

Purpose

Expresses human MDA5 S88D mutant

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MDA5 (IFIH1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S88D

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pRB051_LEU2

Plasmid

#196993

Purpose

Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

Depositor

Matthew Maurano

Article

Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.

Type

Empty backbone

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Expression

Mammalian

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.PHF1

Plasmid

#125167

Purpose

Expresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

PHF1 (PHF1 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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Frt-hspB3

Plasmid

#63094

Purpose

Expression of HSPB3 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb3 (HSPB3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-gp135

Plasmid

#222306

Purpose

Synchronize the trafficking of gp135/podocalyxin from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and gp135 fused to SBP-EGFP (PODXL Rabbit)

Expression

Mammalian

Mutation

signal peptide removed

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-YTHDF2

Plasmid

#38089

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

YTHDF2 YTH domain family, member 2 (YTHDF2 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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