164,852 results

1. pCMV_ABE8e–superNLS

   Plasmid

   #232429

   Purpose

   Expression plasmid for the ABE8e base editor with superNLS

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   ABE8e–superNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUSEss-CHIg-mG1_M18

   Plasmid

   #82357

   Purpose

   Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

   Insert

   mouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   none

   Promoter

   hEF1-HTLV prom

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9-CBE6d

   Plasmid

   #215822

   Purpose

   Express CBE6d (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6d-SpCas9-2xUGI (cas9 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMH-HA

   Plasmid

   #101767

   Purpose

   Gateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion protein

   Depositor

   Michael Huen

   Article

   An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1A-CD19.8H.8TM.BB.3z

   Plasmid

   #200671

   Purpose

   Modular CD8hinge-CD8transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD8HD-CD8TM-41BB-CD3z-P2A-EGFP (CD8A, TNFRSF9, CD247 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-TDP-43 (Q331K)

   Plasmid

   #235488

   Purpose

   Expression of human TDP-43-EGFP with human ALS-causing Q331K mutation

   Depositor

   Maxime Rousseaux

   Article

   Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q331K

   Promoter

   Available since

   April 30, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAAV-FLEX-SaCas9-U6-sgRNA

   Plasmid

   #124844

   Purpose

   Vector for Cre-dependent expression of SaCas9

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SaCas9, gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS and NLS-3xHA

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-MTOR-L1460P

   Plasmid

   #69006

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-L1460P (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Leucine 1460 to Proline

   Promoter

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PKT2/CLP-AKT

   Plasmid

   #20281

   Depositor

   John Ohlfest

   Article

   Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

   Insert

   AKT (AKT1 Human)
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro

    Plasmid

    #205631

    Purpose

    Expression of TnpBmax in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    ISDra2-TnpB optimized for mammalian expression
    

    Use

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. MinLibCas9

    Pooled Library

    #164896

    Purpose

    Human genome-wide CRISPR-Cas9 gRNA library, optimised by mining existing large-scale screening datasets, which provides a >60% reduction in size while preserving key information content

    Depositor

    Mathew Garnett

    Article

    Gonçalves et al Genome Biol. 2021 Jan 21;22(1):40. doi:

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

12. AAV2-hSyn-G-Flamp1

    Plasmid

    #188569

    Purpose

    green biosensor for cAMP in neurons

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1
    

    Use

    AAV

    Tags

    His tag

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-EF1a-Luciferase-IRES-Blast-WPRE

    Plasmid

    #108542

    Purpose

    Lentivirus vector expressing Luciferase with blasticidin resistance

    Depositor

    Javier Alcudia

    Inserts

    Luciferase
    Neomycin
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1apha and PGK

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV pCAG-FLEX-mScarlet-WPRE

    Plasmid

    #99280

    Purpose

    Can be used to generate AAV virus that will express mScarlet in the presence of Cre

    Depositor

    Rylan Larsen

    Insert

    mScarlet
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-Con/Fon-GCaMP6M (AAV8)

    Viral Prep

    #137119-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-EF1a-Con/Fon-GCaMP6M (#137119). In addition to the viral particles, you will also receive purified pAAV-EF1a-Con/Fon-GCaMP6M plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of GCaMP6M. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Tags

    None

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

16. pLAMP1-emiRFP670

    Plasmid

    #136570

    Purpose

    LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    LAMP1-emiRFP670
    

    Use

    Tags

    emiRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF.STAT3C.Ubc.GFP

    Plasmid

    #24983

    Purpose

    3rd generation lentiviral expression of constitutively active Stat3

    Depositor

    Linzhao Cheng

    Article

    Hillion et al Cancer Research 68, 10121, December 2008

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    A662C, N664C (constitutively active)

    Promoter

    Available since

    May 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. Anti-RGS14 [N133/21.2R] (Recombinant Antibody)

    Antibody

    #180088-rAb

    Purpose

    Anti-RGS14 (Rat) recombinant mouse monoclonal antibody

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Recommended Applications

    Immunohistochemistry and Western Blot

    Reactivity

    Mouse and Rat

    Source Species

    Mouse

    Isotype

    IgG2a

    Trial Size

    Available to purchase

    Available since

    March 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

19. pRSF-G1(7FTrp)RS

    Plasmid

    #177310

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV6-Neo-ISG15-LRAA

    Plasmid

    #80405

    Purpose

    overexpression of nonconjugative ISG15

    Depositor

    Isidoro Martinez

    Article

    Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    nonconjugative ISG15, LRAA

    Promoter

    CMV

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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