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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,030 results for: Nes

Showing: 4481 - 4500 of 25030 results

  1. pCIG1_UL136_25kDa_Myc

    Plasmid
    #74954
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)
    Expression
    Mammalian
    Mutation
    delta 1-77, M100A M128A
    Promoter
    CMV, Lentiviral LTR
    Available Since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74954-plasmid-map-sequence-id-141774

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  6. YopH (residues 164-468)

    Plasmid
    #79749
    Purpose
    This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.
    Depositor
    John Chodera, Markus Seeliger, Nicholas Levinson
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    YopH (residues 164-468)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79749-plasmid-map-sequence-id-443904

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  8. pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

    Plasmid
    #218509
    Purpose
    integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mKate2
    Use
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Deletion of Plac_mrfp cassette, introduction of a…
    Promoter
    P23
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218509-plasmid-map-sequence-id-438730

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  9. iOn-CAG∞MCS

    Plasmid
    #154013
    Purpose
    Expression vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with an MCS to clone-in genes of interest and express it from a CAG promoter
    Depositor
    Jean Livet
    Article
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    MCS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    July 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154013-plasmid-map-sequence-id-307168

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  11. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-485302

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  13. pEasyG2_hph

    Plasmid
    #184915
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184915-plasmid-map-sequence-id-367948

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  15. pMax_ScaI_BFP

    Plasmid
    #177824
    Purpose
    Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).
    Depositor
    Leona Samson, Zachary Nagel
    Article
    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111
    Insert
    tagBFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5' inserted ScaI RE cut site
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177824-plasmid-map-sequence-id-348950

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  17. pAcBac1.tR4-AcdRS82

    Plasmid
    #174101
    Purpose
    Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.
    Depositor
    Ryan Mehl
    Article
    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.
    Insert
    M. barkeri tRNA synthetase for acridone82
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N311S, C313G, V366A, W382T
    Promoter
    CMV
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174101-plasmid-map-sequence-id-345718

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  18. dCas9-dMSK1

    Plasmid
    #165602
    Purpose
    Expresses Sp dCas9 fused to truncated human MSK1 (42-802)
    Depositor
    Isaac Hilton
    Article
    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1
    Insert
    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 S. Pyogenes, Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    165602-plasmid-map-sequence-id-327024

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Showing: 4481 - 4500 of 25030 results