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TOPO rtTA3-2A-Cre

Plasmid

#68448

Purpose

TOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

rtTA3-2A-Cre

Use

Gmap

Expression

Bacterial

Mutation

Codon optimized for expression in mice

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPReporter

Plasmid

#65007

Purpose

E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site

Depositor

Mattheos Koffas

Article

Cress et al ACS Synth Biol. 2015 Mar 30.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

none

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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pHHSDKXK (#19)

Plasmid

#35964

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Inserts

Tie2 promoter
SDKLacZ
SV40 pA
Tie2 enhancer

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pMJO-7

Plasmid

#31992

Depositor

Mark Osborn

Article

Osborn et al Hum Gene Ther. 2011 Jun 13.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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NpuDnaEc-cGCaMP2

Plasmid

#34851

Depositor

Kevin Truong

Article

Wong et al Cell Calcium. 2011 Nov 29.

Insert

NpuDnaEc-cGCaMP2

Expression

Bacterial and Mammalian

Promoter

CMV, T7 lac

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pPGK-GZF3-DD

Plasmid

#35093

Depositor

David Segal

Article

Bhakta et al Methods Mol Biol. 2010;649:3-30.

Insert

ZFN GZF3-Fok(DD)

Tags

HA and SV40 NLS

Expression

Mammalian

Mutation

contains the "DD" obligate heterodimer …

Promoter

PGK

Available Since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2 TetO(fl)-3

Plasmid

#27107

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

TetO promoter

Use

Gateway Cloning

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pSems-leader-mXFPe-IFNGR2(30-337)

Plasmid

#192787

Purpose

Expression of mXFPe-tagged IFNGR2 at the plasma membrane, e.g. for single molecule fluorescence microscopy

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

IFNGR2 (IFNGR2 Human)

Tags

Ig k-chain leader sequence and mXFPe

Expression

Mammalian

Mutation

mXFPe: tyrosine 66 to phenylalanine, asparagine 1…

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1-0-GFP

Plasmid

#41662

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

GFP

Expression

Mammalian

Mutation

C-terminal hydrophobic isoleucine residue added a…

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pB7WG_ASX

Plasmid

#98891

Purpose

Plant expression vector for multigene cloning

Depositor

Kristina Gruden

Article

Lukan et al PLoS One. 2018 Jan 4;13(1):e0190526. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMDC-pRPS2-HPB

Plasmid

#32077

Depositor

Fumi Katagiri

Article

Qi et al Plant J. 2009 Mar;57(5):932-44. Epub 200

Type

Empty backbone

Use

Gateway destination binary vector

Tags

HA-Prescission-Biotin

Promoter

RPS2 promoter

Available Since

Dec. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pSicoR-mCh-Chd1i1

Plasmid

#21904

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Insert

Chd1-i1

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF70

Plasmid

#13193

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF70

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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InterCatch-3xL

Plasmid

#186909

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)3 linker and the other side expressing EGFP and puromycin resistance gene

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

TfR-(GGGGS)3-SpyCatcher003-sfCherry1-10

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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BL21ΔBF

Bacterial Strain

#102264

Purpose

lamB, ompF double knockout strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Article

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial Resistance

None

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only
pSGH2

Plasmid

#186706

Purpose

an artificial heat-inducible promoter

Depositor

Baubak Bajoghli, Thomas Czerny

Article

Bajoghli et al Dev Biol. 2004 Jul 15;271(2):416-30. doi

Inserts

GFP
luc

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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LI Intron c1

Plasmid

#54433

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Intron element for construction of silencing vectors (intron1 of AtWRKY33)

Use

Cloning vector

Available Since

July 24, 2014

Availability

Academic Institutions and Nonprofits only

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TAL2084

Plasmid

#39452

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-48-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
Jag2ICD-HA pBacPAK8-3

Plasmid

#17332

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Jagged2 (Jag2 Rat)

Tags

HA

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pPS2351

Plasmid

#1352

Depositor

Pamela Silver

Article

Hitchcock et al Mol Biol Cell 2001 Oct;12(10):3226-41.

Insert

MGA2 (MGA2 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only