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pAAVLINK-EF1alpha-5'-GRIN2B

Plasmid

#244254

Purpose

5' AAVLINK plasmid for GRIN2B expression

Depositor

Zhonghua Lu

Publication

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

GRIN2B (GRIN2B Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVLINKv1-pCALM1-Cre-3' NEXMIF

Plasmid

#236669

Purpose

3' AAVLINK plasmid for NEXMIF expression

Depositor

Zhonghua Lu

Publication

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

NEXMIF (NEXMIF Human)

Use

AAV and Cre/Lox

Tags

FLAG

Promoter

pCALM1

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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DRH015_ AAV-hSyn-WT-THR

Plasmid

#225088

Purpose

Expression of wild-type thyroid receptor beta (THRB, human) with an HA tag in neurons driven by human synapsin promoter.

Depositor

Bernardo Sabatini

Publication

Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

Insert

WT-THRB (THRB Human)

Use

AAV

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCAIP-hXRCC1pd-eGFP

Plasmid

#206033

Purpose

Mammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under the control of a CAG promoter

Depositor

Primo Schaer

Publication

Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

Insert

hXRCC1

Tags

eGFP

Expression

Mammalian

Mutation

S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

Promoter

CAG

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pUDE1084

Plasmid

#168500

Purpose

expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

Depositor

Jean-Marc Daran

Publication

Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

Inserts

Fncas12a
T7RNA polymerase

Use

CRISPR

Promoter

TDH3 and TEF1

Available since

Dec. 16, 2023

Availability

Academic Institutions and Nonprofits only

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Stx5L(m55V)-GFP

Plasmid

#209845

Purpose

Expresses Stx5 M55V mutant fused to GFP in mammalian cells

Depositor

Geert van den Bogaart

Publication

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

STX5 (STX5 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Changed Methionine 55 to Valine, causes loss of S…

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pWPI-LUC7L-3xFLAG

Plasmid

#201640

Purpose

Over-expression of 3xFLAG-tagged human LUC7L

Depositor

Alexis Jourdain, Vamsi Mootha

Publication

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L (LUC7L Human)

Use

Lentiviral

Tags

3xFLAG

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-BACH1t_RFP

Plasmid

#199214

Purpose

BACH1 C-terminus truncated isoform - BACH1t expression plasmid with a RFP reporter on the backbone.

Depositor

Gabor Balazsi

Publication

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Inserts

BACH1t (BACH1 Human)
RFP

Expression

Mammalian

Promoter

CMV and PGK

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-ACSL4-(-706)

Plasmid

#202513

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -706 with respect to exon1 start site)

Depositor

Ming Chen

Publication

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Expression

Mammalian

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S227A]-FLAG

Plasmid

#199318

Purpose

mutated phosphorylation site (MLKL interaction inhibited)

Depositor

Adrian Press

Publication

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 227 to Alanine

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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vYM012_phiC31-nTRE-Ins(high)-pEF1s(high)-H2b-Citine

Plasmid

#197680

Purpose

"Insert(high)-pEF1s(high)" reporter plasmid

Depositor

Michael Elowitz

Publication

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-MBP-CBX1-eReader

Plasmid

#135089

Purpose

Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

Depositor

Marcey Waters

Publication

Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

Insert

CBX1 chromobox protein 1, engineered

Tags

6xhis-tag and MBP

Expression

Bacterial

Mutation

lysine 43 mutated to alanine; aspartic acid 59 mu…

Promoter

T7

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-IDR1-mCherry-Cry2

Plasmid

#177125

Purpose

Express IDR1 fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

Depositor

Wenbo Li

Publication

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (IDR1 domain) (YTHDC1 Human)

Tags

mCherry-CRY2

Expression

Mammalian

Mutation

IDR1 domain only

Promoter

SFFV

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-AviMta3-3xFiBsd

Plasmid

#140962

Purpose

full-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Publication

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis associated 3 (Mta3 Mouse, Synthetic)

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Promoter

CAG

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE053 ZF43x6-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138734

Purpose

Integration Vector, assembled with pHIE045 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pInducer20_SLC38A2_wt

Plasmid

#156182

Purpose

Dox-inducible expression of sgRNA-resistant SLC38A2 wild-type cDNA.

Depositor

Alec Kimmelman

Publication

Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

Insert

SLC38A2 (SLC38A2 Human)

Expression

Mammalian

Mutation

Silent mutations to prevent targeting by sgRNA: T…

Promoter

TRE

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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p35S::mCh-HA-P2A-lifeact-mCh

Plasmid

#135234

Purpose

constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

Depositor

Armin Djamei

Publication

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

mCh-HA-P2A-lifeact-mCh

Tags

HA, lifeact, and p2a

Expression

Plant

Promoter

35S

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-S18

Plasmid

#127827

Purpose

Production of recombinant human ribosomal protein S18

Depositor

Ivanhoe Leung

Publication

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS18 (RPS18 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-L49V

Plasmid

#125844

Purpose

express mutant BAP1-L49V

Depositor

Boyi Gan

Publication

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

L49V

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-BASIC- ARHGAP29 Mutant PROMOTER

Plasmid

#104156

Purpose

luciferase reporter with ARHGAP29 mutant promoter

Depositor

Marius Sudol

Publication

Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

Insert

ARHGAP29 promoter (ARHGAP29 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

delete two TEAD binding sites

Promoter

ARHGAP29

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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