We narrowed to 17,077 results for: Por

1. pAPV-DsRed2-FLAG-ER-5

   Plasmid

   #170622

   Purpose

   Expresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   APV-DsRed2-ER-5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC57-Kan_FR13_APikL2

   Plasmid

   #123648

   Purpose

   Level 0 Golden-Gate compatible vector

   Depositor

   Sophien Kamoun

   Article

   Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

   Insert

   FR13_APikL2
   

   Use

   Golden gate

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Myog'-2A-mCherryNLS-PGK-Puro

   Plasmid

   #69548

   Purpose

   Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

   Depositor

   Charles Gersbach

   Inserts

   2A-mCherry-NLS
   Myogenin 5' homology arm
   Myogenin 3' homology arm (Myog Mouse)
   

   Use

   Mouse Targeting

   Tags

   2x nuclear localization signal and PVT1-2A "…

   Expression

   Mutation

   Silent V205V mutation in myogenin gene to prevent…

   Promoter

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Golgi-DKAR

   Plasmid

   #49790

   Purpose

   FRET-based, Golgi-targeted kinase activity reporter for PKD

   Depositor

   Alexandra Newton

   Article

   Kunkel et al J Biol Chem. 2010 Jul 23;285(30):22748-5

   Insert

   D kinase activity reporter
   

   Use

   Tags

   CFP, YFP, and eNOS(1-33)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pJAT13

   Plasmid

   #18986

   Depositor

   Jay Keasling

   Article

   Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

   Insert

   promoter CP13
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pF(UG) CamKII sf iGluSnFR

   Plasmid

   #158769

   Purpose

   iGluSnFR glutamate sensor containing a Golgi export sequence and and an ER exit motif for improved trafficking to the plasma membrane.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   iGluSnFR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Additional ER and Golgi export sequences

   Promoter

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. M-tdTom-ST1

   Plasmid

   #48678

   Purpose

   Mammalian tdTomato activation reporter for ST1 with GTCCCCTCCACCCCACAGTG protospacer

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, ST1-PAM, and tdTomato reporter. Compatible with S. thermophilus #1 Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Sp6

   Available Since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pBSU6-shScr/CMV-eGFP

   Plasmid

   #89912

   Purpose

   Express shRNA Scrambled control

   Depositor

   Robert Friedlander

   Article

   Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

   Insert

   shRNA Scrambled
   

   Use

   Tags

   eGFP under separate promoter

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBN303

   Plasmid

   #86730

   Purpose

   Tissue-specific FLP expression plasmid for C. elegans

   Depositor

   Peter Askjaer

   Article

   Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

   Insert

   Punc-47::FLP D5
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBI121-Pro-Adh::TaPCS1:cmyc

    Plasmid

    #49765

    Purpose

    Expresses wheat phytochelatin synthase (TaPCS1) in plant expression vector

    Depositor

    Julian Schroeder

    Article

    Gong et al Proc Natl Acad Sci U S A. 2003 Aug 19;10

    Insert

    TaPCS1 (PCS1 Triticum aestivum)
    

    Use

    Plant expression

    Tags

    c-myc

    Expression

    Mutation

    Promoter

    Adh1

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pBN260

    Plasmid

    #86722

    Purpose

    Tissue-specific FLP expression plasmid for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Pmyo-3::FLP D5
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SuperTopAP

    Plasmid

    #20334

    Depositor

    Connie Cepko

    Article

    Cho et al Development. 2006 Aug . 133(16):3167-77.

    Insert

    beta catenin reporter (CTNNB1 Human)
    

    Use

    Ap reporter

    Tags

    AP

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pTXTL-T7max-LuxB

    Plasmid

    #188306

    Purpose

    Expression of LuxB via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    luxB
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. P442

    Plasmid

    #139728

    Purpose

    Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz, promoter is ABA responsive

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pMOD_D_pAtRAB18:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtRAB18

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-C1-PRKAA2ΔC

    Plasmid

    #30307

    Depositor

    Jay Brenman

    Article

    Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

    Insert

    AMP-activated Protein Kinase α2 ΔC (Prkaa1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids Ser 527 to Arg 552

    Promoter

    Available Since

    Aug. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pBN283

    Plasmid

    #86728

    Purpose

    Tissue-specific FLP expression plasmid for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Pdat-1::FLP D5
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAct-dCBEevoAPOBEC1

    Plasmid

    #195514

    Purpose

    Ubiquitous expression of dCBEevoAPOBEC1 in Drosophila

    Depositor

    Michael Boutros

    Article

    Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

    Insert

    dCBE-evoAPOBEC1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCHA1.1-Rap2a-V5

    Plasmid

    #149509

    Purpose

    Stable expression of human Rap2a.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    RAP2 (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Codon Optimized Sequence

    Promoter

    Ubc

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTXTL-T7max-HisGFPHiBiT

    Plasmid

    #188316

    Purpose

    Expression of GFP-HiBiT via T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    GFP-HiBiT fusion
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTXTL-T7max-HiBiTGFPHis

    Plasmid

    #188317

    Purpose

    Expression of HiBiT-GFP via T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    HiBiT-GFP fusion
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge