We narrowed to 10,236 results for: ada

1. pX330-EN479

   Plasmid

   #86234

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nickase and sgRNA against mouse rosa26 locus
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAM4787

   Plasmid

   #120088

   Purpose

   Cyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).

   Depositor

   Susan Golden

   Article

   Chen et al Microbiology. 2016 Dec;162(12):2029-2041

   Insert

   Plasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
   

   Use

   Other

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTF023_His_hsDDA1

   Plasmid

   #174480

   Purpose

   Expresses DDA1 in insect cells

   Depositor

   Eric Fischer

   Article

   Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

   Insert

   DDA1 (DDA1 Human)
   

   Tags

   His-TEV

   Expression

   Insect

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF5B-FRT-control-APEX

   Plasmid

   #73187

   Purpose

   Expresses "control"-APEX (cytosolic) in mammalian cells

   Depositor

   Maxence Nachury

   Article

   Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

   Insert

   control-APEX (Nphp3 Mouse, Glycine max)
   

   Tags

   internal GFP tag

   Expression

   Mammalian

   Mutation

   changed glycine 2 to alanine

   Promoter

   EF1alpha

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXL001-TQM IL13 CAR

   Plasmid

   #154054

   Purpose

   Lentiviral expression of TQM IL13 CAR for CAR-T therapy

   Depositor

   Xiaojun Lian

   Article

   Kim et al Bioact Mater. 2020 May 7;5(3):624-635. d

   Insert

   TQM IL13 CAR
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherry-C1-mTrim21ΔRING-Box

   Plasmid

   #105517

   Purpose

   Expression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cells

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   deletion of RING domain (aa124-462)

   Promoter

   CMV

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

   Plasmid

   #71719

   Purpose

   Expressing a GFP-tagged degron in the soma of C. elegans

   Depositor

   Abby Dernburg

   Article

   Zhang et al Development. 2015 Nov 9. pii: dev.129635

   Insert

   auxin-responsive protein IAA17 (AXR3 Mustard Weed)
   

   Tags

   EmGFP

   Expression

   Worm

   Mutation

   minimal degron sequence (71-114aa) with start cod…

   Promoter

   eef-1A.1 (eft-3)

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRX-IBU-pSu9-HaloTag7-SNAP

   Plasmid

   #217319

   Purpose

   Expresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.

   Depositor

   Noboru Mizushima

   Article

   Yang et al EMBO J. 2024 Nov;43(22):5613-5634. doi:

   Insert

   Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
   

   Use

   Retroviral

   Tags

   HaloTag and SNAP

   Promoter

   CMV

   Available Since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDisplay-GRAB_OT1.0

   Plasmid

   #185384

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   GPCR activation based oxytocin sensor GRAB_OT1.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-C1-mTrim21ΔPRYSPRY

    Plasmid

    #105518

    Purpose

    Expression of mCherry-tagged mouse Trim21 with a deleted PRYSPRY domain in mammalian cells

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deletion of PRYSPRY domain (aa1-281)

    Promoter

    CMV

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid

    #117687

    Purpose

    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

    Depositor

    Erik Sontheimer

    Article

    Gao et al Methods Enzymol. 2019;616:365-383. doi:

    Inserts

    Kanamycin cassette
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET42b-ABE7.10

    Plasmid

    #120398

    Purpose

    Expresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expression

    Depositor

    Junjiu Huang

    Article

    Liang et al Nat Commun. 2019 Jan 8;10(1):67. doi: 10

    Insert

    ABE7.10
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Mammalian codon-optimized

    Promoter

    T7

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3/er-(n2)oxStayGold(c4)

    Plasmid

    #185822

    Purpose

    A soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.

    Depositor

    Atsushi Miyawaki

    Article

    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

    Insert

    er-(n2)oxStayGold(c4)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPS

    Plasmid

    #85801

    Purpose

    Backbone for pooled cloning of Perturb-seq libraries

    Depositor

    Aviv Regev

    Article

    Dixit et al Cell. 2016 Dec 15;167(7):1853-1866.e17.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-HA-DEPTOR-WT

    Plasmid

    #159159

    Purpose

    Expresses DEPTOR in mammalian cells

    Depositor

    Michele Pagano

    Article

    Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

    Insert

    DEPTOR (DEPTOR Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-AviTag-DogTag-MBP

    Plasmid

    #171773

    Purpose

    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    AviTag-DogTag-MBP
    

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Peptide tag added to N-terminus of MBP via a GSGE…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. vDip-C: pAAV-Pcp2-mGreenLantern-KASH

    Plasmid

    #207613

    Purpose

    vDip-C AAV vector with mouse Pcp2 (also known as L7) promoter driving green fluorophore mGreenLantern with a KASH nuclear membrane tag to isolate cell nuclei of cerebellar Purkinje cells

    Depositor

    Longzhi Tan

    Article

    Tan et al Science. 2023 Sep 8;381(6662):1112-1119.

    Insert

    mGreenLantern
    

    Use

    AAV

    Tags

    KASH

    Promoter

    mouse Pcp2 promoter (L7-6)

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC_N_GATA4

    Plasmid

    #167758

    Purpose

    Expresses GATA4 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    GATA4 (GATA4 Human)
    

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMK395 (AAVS1 CMV-OsTIR1(F74A))

    Plasmid

    #159992

    Purpose

    Expression of OsTIR1(F74A) in human cells

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-OsTIR1(F74A)
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. XE251 pCDNA3.1(zeo)-hDsh3

    Plasmid

    #16758

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-hDsh3 (DVL3 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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