We narrowed to 70,800 results for: nin

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pREXC3GH

Plasmid

#47077

Purpose

FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Lactococcus lactis expression

Tags

3C protease site, GFP, and His

Promoter

NIS (nisin)

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMEXC3H

Plasmid

#110080

Purpose

Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-HA-OTUB2

Plasmid

#22552

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

OTUB2 (OTUB2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pWS481

Plasmid

#123029

Purpose

LexA UAS

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (6x) [2a]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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p8266 LentiCRISPR v2 hygro sgNT-1

Plasmid

#193977

Purpose

Expression of spCas9 and non-targeting control sgRNA

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9

Use

Lentiviral

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1a-CRISPRoffv2.1

Plasmid

#207174

Purpose

Gateway Cloning Vector for CRISPRoffv2.1

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

CRISPRoffv2.1

Use

CRISPR

Tags

TagBFP

Expression

Mammalian

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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PB-hEF1a-MG

Plasmid

#175504

Purpose

All-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elements

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

hEF1a

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1310

Plasmid

#61253

Purpose

Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

U6 promoter

Use

CRISPR; Cloning template

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mRFP-wGBD

Plasmid

#26733

Depositor

William Bement

Article

Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Insert

Neuronal Wiskott-Aldrich Syndrome protein (WASL Human)

Use

Reporter for cdc42

Tags

mRFP

Expression

Mammalian

Mutation

Cdc42 binding domain of N-WASP

Available Since

April 21, 2011

Availability

Academic Institutions and Nonprofits only
pPB-EF1a-MegaGate-DD-Hygro

Plasmid

#175267

Purpose

MegaGate destination with C-terminal DD tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

Destabilization Domain

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pOSIP-TH (TetR, HK022)

Plasmid

#45988

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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EcAvs4

Plasmid

#188794

Purpose

Avs4 from Escherichia coli NCTC11132

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs4

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIP-EGFP-APEX2-V5-Giantin

Plasmid

#236404

Purpose

Expresses a fluorescent Golgi-localized APEX2

Depositor

Jared Rutter

Article

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

EGFP-APEX2-V5-Giantin

Use

Retroviral

Tags

APEX2, EGFP, and V5

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGGF007

Plasmid

#48847

Purpose

Provides a kanamycin resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pNOS::KanamycinR::tNOS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-HA-PSMD7

Plasmid

#22558

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

PSMD7 (PSMD7 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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isce rfp/cfp

Plasmid

#83323

Purpose

fluorescent Isce joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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EpAvs1

Plasmid

#188788

Purpose

Avs1 from Erwinia piriflorinigrans CFBP5888

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EpAvs1

Expression

Bacterial

Promoter

bla

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-HA-Hygro

Plasmid

#175268

Purpose

MegaGate destination with C-terminal HA tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-3P2A

Plasmid

#111772

Purpose

Quadcistronic construct: Co-express four genes by 3 P2A peptides

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Type

Empty backbone

Use

Cloning intermediate

Promoter

T7

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRF-AmtR

Plasmid

#49355

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

AmtR repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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