We narrowed to 23,749 results for: promoter

1. pZmUbi-5'UTR

   Plasmid

   #154057

   Purpose

   Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   Promoter and 5' UTR Ubiquitin (Zea mays)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   N/A

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFA6a-kanMX6-PGAL1

   Plasmid

   #41605

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Available Since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pT7-SpCas9_sgRNA-site1 (RTW443)

   Plasmid

   #160136

   Purpose

   T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Promoter

   T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GG-EBNA-OCT4

   Plasmid

   #69537

   Purpose

   Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   5 concatenated gRNA transcriptional cassettes targeting OCT4
   

   Use

   CRISPR

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 3xFLAG-dCas9/pMXs-IG

   Plasmid

   #51258

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pL0-MtBCP1Pro

   Plasmid

   #159415

   Purpose

   Medicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Insert

   MtBCP1 Promoter
   

   Use

   Puc19-derived

   Expression

   Bacterial

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHes1-GFP (CC#109)

   Plasmid

   #15133

   Depositor

   Connie Cepko

   Article

   Furukawa et al Neuron. 2000 May . 26(2):383-94.

   Insert

   Hes1 (Hes1 Rat)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pUC-hU6-crScaffold_SV40-BFP

   Plasmid

   #224860

   Purpose

   crRNA scaffold for RfxCas13d expressed from hU6 promoter and reporter BFP protein expressed from SV40 promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRK-flag-USP19 C506S

   Plasmid

   #78584

   Purpose

   Expression flag-USP19 C506S in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 C506S (USP19 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   mutate 506 cysteine residue to serine

   Promoter

   CMV

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pGEX-p53 R175H

    Plasmid

    #39480

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Insert

    TP53 (TP53 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    P72R and R175H

    Available Since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pLPP3

    Plasmid

    #209965

    Purpose

    Contains Level 0 Part: constitutive Promoter (P25) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P25)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pLPP7

    Plasmid

    #209969

    Purpose

    Contains Level 0 Part: autoinducible Promoter (PfabZ) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P_fabZ)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pLPP8

    Plasmid

    #209970

    Purpose

    Contains Level 0 Part: autoinducible Promoter (PgadB) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P_gadB)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pLPP9

    Plasmid

    #209971

    Purpose

    Contains Level 0 Part: constitutive Promoter (PldhD) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P_ldhD)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pLPP1

    Plasmid

    #209963

    Purpose

    Contains Level 0 Part: mock / negative control Promoter (P-nc) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (mock)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pLPP2

    Plasmid

    #209964

    Purpose

    Contains Level 0 Part: constitutive Promoter (P48) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P48)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pXPR_212

    Plasmid

    #133457

    Purpose

    U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-p53

    Plasmid

    #39479

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Insert

    TP53 (TP53 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    P72R

    Available Since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pXPR_213

    Plasmid

    #133456

    Purpose

    H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    Control guide
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. USP19 sgRNA2

    Plasmid

    #78586

    Purpose

    delete USP19 gene in human cell

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    USP19 sgRNA2 (USP19 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only