We narrowed to 20,059 results for: ato

1. pL0_EF-HO

   Plasmid

   #202170

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 5902 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   HO terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pL0_EF-NRT

   Plasmid

   #202169

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 54101 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   NRT terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pL0_EF-sit4

   Plasmid

   #202168

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 48707 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   sit4 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pL0_EF-CaMK2

   Plasmid

   #202167

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 39236 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   CaMK2 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pL0_EF-fbaC5

   Plasmid

   #202166

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 41423 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   fbaC5 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pL0_EF-OEE3

   Plasmid

   #202165

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 54499 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   OEE3 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pL0_EF-27877

   Plasmid

   #202162

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 27877 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   27877 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pL0_EF-51183

   Plasmid

   #202161

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 51183 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   51183 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pL0_EF-GEF

   Plasmid

   #202160

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 41365 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   GEF terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pL0_EF-47740

    Plasmid

    #202159

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 47740 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    47740 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pL0_EF-BCA

    Plasmid

    #202158

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 51305 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    BCA terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. FUW-MD-mTurquoise

    Plasmid

    #193094

    Purpose

    constitutive expression of the mitosis degradation (MD) domain of cyclin B1 fused to mTurquoise in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

    Insert

    MD-mTurquoise
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTP-PCR C1

    Plasmid

    #189618

    Purpose

    Cloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

    Insert

    Mitochondrial Genome of Thalassiosira pseudonana
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Minor additional mutations described in manuscript

    Promoter

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-RIPK3[K5A]-FLAG

    Plasmid

    #199326

    Purpose

    mutated ubiquitination site (ubiquitination inhibited)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Lysine 5 to Alanine

    Promoter

    Available since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-RIPK3[K5R]-FLAG

    Plasmid

    #199325

    Purpose

    mutated ubiquitination site (ubiquitination active)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Lysine 5 to Arginine

    Promoter

    Available since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA 3.1-nsp7

    Plasmid

    #201026

    Purpose

    Expresses SARS-CoV-2 nsp7 in mammalian.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CMV EGFP-PPM1H-H153D

    Plasmid

    #200432

    Purpose

    Mammalian expression of H153D mutation in PPM1H

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    PPM1H protein phosphatase, Mg2+/Mn2+ dependent 1H
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    H153D

    Promoter

    CMV

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBAD-P19-EGFP

    Plasmid

    #196609

    Purpose

    siRNA vector expressing TMV P19 and a 720-long EGFP sequence as inverted repeat

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Insert

    p19 + EGFP inverted repeat sequence
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    AraC

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBAD-P19-NP

    Plasmid

    #196608

    Purpose

    siRNA vector expressing TMV P19 and a 425bp-long NP sequence as inverted repeat

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Insert

    p19 + Influenza virus NP inverted repeat
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTP-PCR C4

    Plasmid

    #189621

    Purpose

    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

    Insert

    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Repeat region removed, and minor additional mutat…

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTP-PCR C2

    Plasmid

    #189619

    Purpose

    Cloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

    Insert

    Mitochondrial Genome of Thalassiosira pseudonana
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Minor additional mutations described in manuscript

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMX-Mef2c-puro

    Plasmid

    #198228

    Purpose

    Retroviral expression of Mef2c

    Depositor

    Li Qian

    Article

    Wang et al Cell Stem Cell. 2022 Oct 6;29(10):1491-1

    Insert

    Mef2c (Mef2c Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lenti-guide-puro mYwhae -2

    Plasmid

    #198498

    Purpose

    lentiviral stable expression of mYwhae gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mYwhae (Ywhae Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Lenti-guide-puro mYwhae - 1

    Plasmid

    #198497

    Purpose

    lentiviral stable expression of mYwhae gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mYwhae (Ywhae Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDGO186N-KS1

    Plasmid

    #174301

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDGO186N-KS2

    Plasmid

    #174302

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. OA-1085L

    Plasmid

    #191376

    Purpose

    pBac-U6-crGFP-array-UTR-opie2-eGFP-3xp3-tdTomato

    Depositor

    Omar Akbari

    Article

    Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

    Insert

    4 gRNAs targeting GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pXD70Tet(DadRmt4)-DadR(linker)

    Plasmid

    #191639

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(linker region shuffled) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Linker region sequence shuffled

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pXD70Tet(DadR-NFLAG)-N-3xFLAG-DadR

    Plasmid

    #191640

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXD70Tet(DadRmt5)-N-3xFLAG-DadR(ΔDBD)

    Plasmid

    #191641

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔDBD) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    ΔDBD

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pXD70Tet(DadRmt6)-N-3xFLAG-DadR(ΔTM)

    Plasmid

    #191642

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔTM) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    ΔTM

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pXD70Tet(DadRmt7)-N-3xFLAG-DadR(linker)

    Plasmid

    #191643

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(linker region shuffled) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Linker region sequence shuffled

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pXD70Tet(DadH)-Pdadh-DadhABC(A2)

    Plasmid

    #191644

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    Dopamine dehydroxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDe_Cas9_KANA_R160a

    Plasmid

    #173756

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160a

    Depositor

    Kristina Gruden

    Article

    Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

    Insert

    Cas9_sgRNA1/R160a_sgRNA2/R160a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6 &StU6

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDe_Cas9_KANA_R160b

    Plasmid

    #173757

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160b

    Depositor

    Kristina Gruden

    Article

    Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

    Insert

    Cas9_sgRNA1/R160b_sgRNA2/160b
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6 &StU6

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDe_Cas9_KANA_R390a

    Plasmid

    #173758

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA390a

    Depositor

    Kristina Gruden

    Article

    Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

    Insert

    Cas9_sgRNA1/390a_sgRNA2/390a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6 &StU6

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. nPPARg429-gs10-NLS-VPR

    Plasmid

    #188266

    Purpose

    Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    nPPARg429 (PPARG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDGO186N-KS3

    Plasmid

    #174303

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pYPQ265N2

    Plasmid

    #174001

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    rAPOBEC1-zCas9(D10A)-SpRY-UNG
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pYPQ265O2

    Plasmid

    #174002

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pYPQ265L2

    Plasmid

    #174000

    Purpose

    Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLenti-Zyxin-E (aa1-138)-EGFP

    Plasmid

    #187697

    Purpose

    Visualization of Zyxin

    Depositor

    Mary Beckerle

    Article

    Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

    Insert

    Zyxin (Zyx Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa1-138

    Promoter

    CMV

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLenti-Zyxin-G (aa1-372)-EGFP

    Plasmid

    #187699

    Purpose

    Visualization of Zyxin

    Depositor

    Mary Beckerle

    Article

    Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

    Insert

    Zyxin (Zyx Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa1-372

    Promoter

    CMV

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLenti-Zyxin-F (aa373-564)-EGFP

    Plasmid

    #187698

    Purpose

    Visualization of Zyxin

    Depositor

    Mary Beckerle

    Article

    Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

    Insert

    Zyxin (Zyx Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa373-564

    Promoter

    CMV

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLenti-Zyxin-C (aa139-371)-EGFP

    Plasmid

    #187695

    Purpose

    Visualization of Zyxin

    Depositor

    Mary Beckerle

    Article

    Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

    Insert

    Zyxin (Zyx Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa139-371

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLenti-Zyxin-A (aa139-564)-EGFP

    Plasmid

    #187693

    Purpose

    Visualization of Zyxin

    Depositor

    Mary Beckerle

    Article

    Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

    Insert

    Zyxin (Zyx Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa139-564

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pLenti-Zyxin-B (aa309-564)-EGFP

    Plasmid

    #187694

    Purpose

    Visualization of Zyxin

    Depositor

    Mary Beckerle

    Article

    Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

    Insert

    Zyxin (Zyx Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa309-564

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pTE1069

    Plasmid

    #186629

    Purpose

    SCB-GFP E. coli reporter plasmid. Encodes repressor ScbR and ScbAp promoter upstream of GFP.

    Depositor

    Eriko Takano

    Article

    Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10

    Inserts

    scbR
    scbAp promoter
    

    Use

    Synthetic Biology

    Tags

    6xArg

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23100 and scbAp

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pILGFP4D41

    Plasmid

    #185841

    Purpose

    Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pILGFP10CF72 (B11)

    Plasmid

    #185859

    Purpose

    Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge