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mCardinal-H2B-C-10

Plasmid

#56162

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H2B (H2BC11 Human)

Tags

mCardinal

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pSMALB

Plasmid

#161786

Purpose

Gateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and TagBFP.

Depositor

John Dick, Peter van Galen

Article

van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Promoter

Bidirectional minhCMV and SFFV

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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PB-U6-DR-SgRNA-DR-EF1a-mCherry-SV40

Plasmid

#154002

Purpose

Empty SgRNA expression system matching CasRx

Depositor

Hui Yang

Article

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Insert

SgRNA expression system

Use

CRISPR

Promoter

U6, EF1a

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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amilCP_Pink chromoprotein

Plasmid

#117851

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilCP_Pink

Use

Synthetic Biology; Escherichia coli

Mutation

64A,65C, BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti HRE mutant-Luc pGK Hygro

Plasmid

#118707

Purpose

reporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferase

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

HREmutant-Luc

Use

Lentiviral

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-DEST

Plasmid

#106348

Purpose

Destination vector for gateway cloning. Developed for Flp-In T-REx system.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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I48 ELP

Plasmid

#68937

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residue

Depositor

Andrew MacKay

Article

Shi et al J Control Release. 2013 Nov 10;171(3):33

Insert

I48 ELP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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P2-ECFP-pA (Construct 6)

Plasmid

#55198

Purpose

Synthetic promoter responsive to gRNA2

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

mCerulean

Use

Synthetic Biology

Expression

Mammalian

Promoter

Minimal Ade MLP

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pHTIKA

Plasmid

#22062

Depositor

Maria Castro

Article

Xiong et al J Virol. 2006 Jan . 80(1):27-37.

Insert

hCMV-TetOn expression cassette

Expression

Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
ovoD1 D1B2H (7.2 kb)

Plasmid

#37282

Depositor

Norbert Perrimon

Article

Chou et al Development. 1993 Dec;119(4):1359-69.

Insert

ovoD1 genomic DNA fragment

Expression

Insect

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
ovoD1 D1B2R#225 (10 kb)

Plasmid

#37281

Depositor

Norbert Perrimon

Article

Chou et al Development. 1993 Dec;119(4):1359-69.

Insert

ovoD1 genomic DNA fragment

Expression

Insect

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pFL-TK-AID3'UTR-MUT

Plasmid

#19987

Depositor

Nina Papavasiliou

Article

Teng et al Immunity. 2008 May . 28(5):621-9.

Insert

AID 3' UTR (Aicda Mouse)

Expression

Mammalian

Mutation

Mutation

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pFL-TK-AID3'UTR-DEL

Plasmid

#19988

Depositor

Nina Papavasiliou

Article

Teng et al Immunity. 2008 May . 28(5):621-9.

Insert

AID 3' UTR (Aicda Mouse)

Expression

Mammalian

Mutation

Deletion

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-eGFP-2A-mCherry_D4H

Plasmid

#194875

Purpose

Ratiometric D4H sensor for in vivo neuron-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-D4H

Use

AAV

Expression

Mammalian

Promoter

human Synapsin

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt-S473A

Plasmid

#49190

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

S473A

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only
pFA6a-GST-kanMX6

Plasmid

#39293

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-GFP

Plasmid

#39290

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-RapR-p38

Plasmid

#25935

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-p38 (Mapk14 Mouse, Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 26, 2010

Availability

Academic Institutions and Nonprofits only

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BFP

Plasmid

#201383

Purpose

Control nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

BFP

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pF1-Hs Cdk1/cyclin B1

Plasmid

#177330

Purpose

Co-expression of the human active Cdk1/cyclin B1 kinase complex

Depositor

Andreas Boland

Article

Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

Inserts

Cyclin-dependent kinase 1 (CDK1 Human)
G2/mitotic-specific cyclin-B1 (CCNB1 Human)

Tags

2 x Strep-tag and 8 x His

Expression

Insect

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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