Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 10,024 results for: CAG

Showing: 4561 - 4580 of 10024 results

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pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1.16

Plasmid

#212532

Purpose

Encodes SARS-CoV-2 variant XBB.1.16 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike XBB.1.16 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 HK.3

Plasmid

#212572

Purpose

Encodes SARS-CoV-2 spike for HK.3 pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike HK.3 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Ai65(RCFL-tdT) targeting vector

Plasmid

#61577

Purpose

Target a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

pCAG-FSF-LSL-tdTomato

Use

Cre/Lox and Mouse Targeting

Promoter

CAG

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 JN.1

Plasmid

#216526

Purpose

Encodes SARS-CoV-2 spike for JN.1 pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike JN.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1.5

Plasmid

#212523

Purpose

Encodes SARS-CoV-2 variant XBB.1.5 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike XBB.1.5 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.5

Plasmid

#212505

Purpose

Encodes SARS-CoV-2 variant BA.5 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.5 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-DIO-mCherry-nega miR

Plasmid

#137732

Purpose

Lentivirus vector for control miR in a Cre-dependent manner

Depositor

Tomoyuki Furuyashiki

Article

Nie et al Neuron. 2018 Aug 8;99(3):464-479.e7. doi

Insert

control microRNA

Use

Lentiviral

Tags

mChherry

Expression

Mammalian

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 LB.1

Plasmid

#223277

Purpose

Encodes SARS-CoV-2 spike LB.1 for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike LB.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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PB-PE SpRY

Plasmid

#179082

Purpose

NOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…

Promoter

CAG

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 JN.1.11.1

Plasmid

#223272

Purpose

Encodes SARS-CoV-2 spike JN.1.11.1 for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike JN.1.11.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1

Plasmid

#212522

Purpose

Encodes SARS-CoV-2 variant XBB.1 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike XBB.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1025

Plasmid

#235705

Purpose

Expression of FL human VPS34 and human VPS15-ZZ in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Inserts

VPS34 (PIK3C3 Human)
VPS15 (PIK3R4 Human)

Tags

3xTEV-ZZ

Expression

Mammalian

Promoter

CAG and CMV

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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Ai27

Plasmid

#34630

Purpose

Cre-dependent expression of hChR2(H134R)-tdTomato, for optogenetic excitation

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

Insert

Floxed hChR2(H134R)-tdTomato

Use

Mouse Targeting

Tags

tdTomato

Mutation

His 134 has been mutated to Arg

Promoter

CAG

Available Since

Feb. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 EG.5.1

Plasmid

#212454

Purpose

Encodes SARS-CoV-2 variant EG.5.1 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike EG.5.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 XBB

Plasmid

#212521

Purpose

Encodes SARS-CoV-2 variant XBB Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike XBB (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.2

Plasmid

#212487

Purpose

Encodes SARS-CoV-2 variant BA.2 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.2 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.2.86

Plasmid

#212538

Purpose

Encodes SARS-CoV-2 variant BA.2.86 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.2.86 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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PB-PE SpG

Plasmid

#179081

Purpose

NOTE: An improved version of this plasmid is now available. See Addgene plasmid #242072. All-in-one prime editor piggyBac transposon, SpG variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R

Promoter

CAG

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pPiggyBac_NTVE(PABP)

Plasmid

#254463

Purpose

Transposon encoding doxycycline-inducible NTVE(PABP). Co-expression of PiggyBac transposase required. Tet-On 3G and green fluorescent protein mGreenLantern are co-expressed via the CAG promoter.

Depositor

Gil Westmeyer

Article

Armbrust et al Nat Commun. 2026 Apr 25;17(1):3812. doi:

Insert

NTVE(PABP)

Expression

Mammalian

Promoter

TRE3G

Available Since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pPiggyBac_NTVE(PABP)-dVSVG-HA

Plasmid

#254462

Purpose

Transposon encoding doxycycline-inducible NTVE(PABP) with HA tag. Co-expression of PiggyBac transposase required. Tet-On 3G and puromycin N-acetyltransferase are co-expressed via CAG promoter.

Depositor

Gil Westmeyer

Article

Armbrust et al Nat Commun. 2026 Apr 25;17(1):3812. doi:

Insert

NTVE(PABP)_P2A_dVSV-G-HA

Expression

Mammalian

Promoter

TRE3G

Available Since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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Showing: 4561 - 4580 of 10024 results

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