We narrowed to 6,891 results for: GFP expression plasmids

1. pmCellFree_KA1_SARS-COV-2_ORF8

   Plasmid

   #169395

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus ORF8 with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _ORF8 (ORF8 SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Expression

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCellFree_KA1_SARS-COV-2 _NSP1

   Plasmid

   #169376

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus NSP1 with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _NSP1
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Expression

   Mutation

   Codon optimized for Saccharomyces cerevisiae

   Promoter

   T7- CMV/CAG

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCellFree_KA1_SARS-COV-2_ORF6

   Plasmid

   #169392

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus ORF6 with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _ORF6 (ORF6 SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Expression

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEG608

   Plasmid

   #40194

   Depositor

   Geraldine Seydoux

   Article

   Griffin et al Cell. 2011 Sep 16;146(6):955-68.

   Insert

   Mex-5 (RNAi resistant) (mex-5 Nematode)
   

   Use

   Tags

   3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…

   Expression

   Worm

   Mutation

   Exon 2 recoded to be RNAi-resistant

   Promoter

   4.4 kb mex-5 promoter fragment

   Available since

   Sept. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pPyPGK-Myc-LaG17-SynNotch-tTA-IRES-Ble

   Plasmid

   #183607

   Purpose

   Mammalian expression vector: mouse Pgk1 promoter driving expression of a SynNotch receptor comprising anti-GFP nanobody (LaG17), mouse Notch1 core, tTA. Confers bleomycin/zeocin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   LaG17 GFP nanobody SynNotch tTA
   

   Use

   Tags

   Myc and human CD8a signal sequence

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Pgk1

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCellFree_KA1_SARS-COV-2_ORF3a

   Plasmid

   #169390

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3a with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _ORF3a (ORF3a SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Expression

   Mutation

   Codon optimized for Saccharomyces cerevisiae

   Promoter

   T7- CMV/CAG

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCellFree_KA1_SARS-COV-2_ORF7b

   Plasmid

   #169394

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7b with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _ORF7b (ORF7b SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Expression

   Mutation

   Codon optimized for Saccharomyces cerevisiae

   Promoter

   T7- CMV/CAG

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p189

   Plasmid

   #65207

   Purpose

   Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.

   Depositor

   LuZhe Sun

   Article

   Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Premature stop codon added to pGEM5Z(+)-EGFP (TGG…

   Promoter

   CMV

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

9. GuideVector_BbsI

   Plasmid

   #110692

   Purpose

   For rapid cloning and expression of sgRNAs in mammalian cells. Contains copGFP for easy FACS sorting.

   Depositor

   Douglas Mahoney

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCellFree_KA1_SARS-COV-2_ORF7a

    Plasmid

    #169393

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7a with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _ORF7a (ORF7a SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAG-Blimp1

    Plasmid

    #73995

    Purpose

    Plasmid expressing mouse Blimp1

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    Blimp1 gene (Prdm1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAB1

    Plasmid

    #62547

    Purpose

    broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.

    Depositor

    Michael Franklin

    Article

    Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47

    Insert

    eGFP
    

    Use

    Broad host range plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMBA337

    Plasmid

    #214750

    Purpose

    Medium copy thyA-infA plasmid containing bicistronic gfp-specR for use in mouse work validation

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3-RBS(4533)-specR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-XL-AncBE4max

    Plasmid

    #136254

    Purpose

    Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max

    Depositor

    Zhaohui Ye

    Article

    McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

    Insert

    AncBE4max_GFP
    

    Use

    CRISPR; Piggybac dna transposon

    Tags

    Expression

    Mammalian

    Mutation

    The AncBE4max_GFP insert was PCR amplified from a…

    Promoter

    TRE3G

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-AAV-sh[control]

    Plasmid

    #75438

    Purpose

    control shRNA vector

    Depositor

    Edward Burton

    Article

    Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

    Insert

    GFP
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR- flag-Lag16-iCAM-1-Tether

    Plasmid

    #205196

    Purpose

    This vector encodes of the synCAM Tether (iCAM1) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM iCAM1 Tether and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. attB53_SNCB+_attB53

    Plasmid

    #183614

    Purpose

    RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Inserts

    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    

    Use

    Rmce

    Tags

    Myc and human CD8a signal sequence

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-flag-Lag16-fibcon-iCAM1-tether

    Plasmid

    #205213

    Purpose

    This vector encodes of the synCAM tether (fibcon - iCAM1) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM fibcon - iCAM1 Tether and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. attB53_SNCB-_attB53

    Plasmid

    #183615

    Purpose

    RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Inserts

    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    

    Use

    Rmce

    Tags

    Myc and human CD8a signal sequence

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmCellFree_KA1_SARS-COV-2_RBD -N501Y

    Plasmid

    #169407

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 mutated RBD domain (N510Y, UK strain) of S protein with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _RBD (N501Y) (S SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    N501Y

    Promoter

    T7- CMV/CAG

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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