We narrowed to 611 results for: prime editing

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HEK3_pegF_18bp

Plasmid

#174852

Purpose

Express forward pegRNA mediating 991-bp deletion and 18-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegF_18bp insertion

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegR_18bp

Plasmid

#174853

Purpose

Express reverse pegRNA mediating 991-bp deletion and 18-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegR_18bp

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegF_44bp

Plasmid

#174854

Purpose

Express forward pegRNA mediating 991-bp deletion and 44-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegF_44bp insertion

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegR_44bp

Plasmid

#174855

Purpose

Express forward pegRNA mediating 991-bp deletion and 44-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegR_44bp insertion

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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ATP1A1_T804N_hPGK1_mScarlet-I_Donor

Plasmid

#173207

Purpose

HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I cassette to ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1-T804N mScarlet-I donor

Use

CRISPR

Expression

Mammalian

Promoter

hPGK1

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pU6nH1-HD10kb

Plasmid

#172657

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-HD10kbA/pegRNA-HD10kbB

Use

CRISPR

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-L858R-T790M-dual-epegRNA

Plasmid

#214100

Purpose

Lentiviral vector expressing epegRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two epegRNAs using independent U6 promoters.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR L858R epegRNA/EGFR T790M epegRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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AID-NG-CBE

Plasmid

#227416

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

AID-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AID-N51A-NG-CBE

Plasmid

#227417

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

AID-N51A-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-L227R-ngRNA+15_EF1a-puroR

Plasmid

#207360

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

L227R-CFTR +15 ngRNA

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-hP53DD (TET+CTD)

Plasmid

#214082

Purpose

Mammalian expression tetramerization domain (TET) and C-terminal negative regulatory domain (CTD) of human TP53

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

hP53DD (TP53 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP

Plasmid

#172670

Purpose

For expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

SDHB pegRNA and SDHB_nick-sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry

Plasmid

#177183

Purpose

plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

sgRNA targeting the PEAR-GFP plasmid

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pX330_ATP1A1_G7

Plasmid

#173204

Purpose

Expresses the ATP1A1 G7 sgRNA in combination with SpCas9 to target ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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ATP1A1_T804N_hPGK1_EBFP_Donor

Plasmid

#187455

Purpose

HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the EBFP cassette to ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1-T804N EBFP donor (ATP1A1 Human)

Expression

Mammalian

Promoter

hPGK1

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_ATP1A1-G7

Plasmid

#173203

Purpose

Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pegRNA_LMNA-1

Plasmid

#199273

Purpose

pegRNA used to induce LMNA (c.1824C > T) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LMNA 1824CtoT pegRNA

Expression

Mammalian

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pegRNA_LRRK2-3

Plasmid

#199272

Purpose

pegRNA used to induce LRRK2 (c. 6055 G > A) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LRRK2 6055GtoA pegRNA

Expression

Mammalian

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Rosa26-twinPE-attB

Plasmid

#207859

Purpose

AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PEmaxDRNaseH-dualU6-Rosa26-twinPE-attB

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pvPE-V3-Puro

Plasmid

#240289

Purpose

Encodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene in to mammalian cells for gene editing.

Depositor

Lusheng Huang

Article

Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

Insert

pvPE-V3-Puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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