We narrowed to 16,918 results for: Emb;

1. TDP-43_NTD1-80_E17R

   Plasmid

   #104479

   Purpose

   bacterial expression of TDP-43 NTD E17R

   Depositor

   Nicolas Fawzi

   Article

   Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E17R

   Promoter

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKTop::tse5-CT (1169-1281)

   Plasmid

   #192955

   Purpose

   For membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   tse5-CT (1169-1281)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1281)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDH_CMV_HMGA_linker_mKate2

   Plasmid

   #167335

   Purpose

   Expresses mKate2 fused to TFAM HMGA+linker mutant in mammalian cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Lentiviral

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   Truncation mutant containing MTS (1-42) as well a…

   Promoter

   CMV

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKTop::sptse5-CT (1169-1229)

   Plasmid

   #192948

   Purpose

   For membrane insertion study; expresses Tse5-CT (1169-1229)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT (1169-1229)
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1229)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKTop::tse5-CT (1169-1300)

   Plasmid

   #192956

   Purpose

   For membrane insertion study; Expresses tse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   tse5-CT (1169-1300)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1300)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKTop::tse5-CT (1169-1317)

   Plasmid

   #192957

   Purpose

   For membrane insertion study; Expresses tse5-CT (1169-1317) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   tse5-CT (1169-1317)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1317)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKTop::tse5-CT (1169-1269)

   Plasmid

   #192954

   Purpose

   For membrane insertion study; Expresses tse5-CT (1169-1269) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   tse5-CT (1169-1269)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1269)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKTop::tse5-CT (1169-1229)

   Plasmid

   #192953

   Purpose

   For membrane insertion study; Expresses tse5-CT (1169-1229) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   tse5-CT (1169-1229)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1229)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKTop::sptse5-CT (1169-1281)

   Plasmid

   #192950

   Purpose

   For membrane insertion study; Expresses sptse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT (1169-1281)
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1281)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKTop::sptse5-CT (1169-1269)

    Plasmid

    #192949

    Purpose

    For membrane insertion study; Expresses Tse5-CT (1169-1269)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    sptse5-CT (1169-1269)
    

    Use

    Tags

    PelB signal peptide

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1269)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKTop::sptse5-CT (1169-1300)

    Plasmid

    #192951

    Purpose

    For membrane insertion study; Expresses sptse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    sptse5-CT (1169-1300)
    

    Use

    Tags

    PelB signal peptide

    Expression

    Bacterial

    Mutation

    encodes for sptse5 (1169-1300)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-Duet1-6xHis-TEV-NDP52

    Plasmid

    #190194

    Purpose

    Plasmid for the protein expression of 6xHis-TEV-NDP52 in a bacterial expression system. Internal reference: SMC401

    Depositor

    Sascha Martens

    Article

    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDH_CMV_dimer_mKate2

    Plasmid

    #167333

    Purpose

    Expresses mKate2 fused to TFAM no-dimerization mutant in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Changed K95A, Y99F, E106A, E112A and R116A

    Promoter

    CMV

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDH_CMV_HMGA_mKate2

    Plasmid

    #167334

    Purpose

    Expresses mKate2 fused to TFAM HMGA mutant in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Truncation mutant containing MTS (1-42) and HMGA …

    Promoter

    CMV

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDH_CMV_dC_mKate2

    Plasmid

    #167336

    Purpose

    Expresses mKatet2 fused to TFAM dC-tail mutant in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Truncation mutant containing MTS (1-42) as well a…

    Promoter

    CMV

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDH_CMV_L6_mKate2

    Plasmid

    #167332

    Purpose

    Expresses mKate2 fused to TFAM L6 mutant in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Changed K136, H137, K139, R140, K146 and K147 to …

    Promoter

    CMV

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti C-MYC-DDK Puro PNRC1-W300A

    Plasmid

    #123301

    Purpose

    Mammalian expression of PNRC1-W300A mutant C-MYC-FLAG

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    PNRC1 (PNRC1 Human)
    

    Use

    Lentiviral

    Tags

    MYC-FLAG

    Expression

    Mammalian

    Mutation

    W300A

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX6p1-Rbck1

    Plasmid

    #63135

    Purpose

    Expression of human RBCK1/HOIL-1L

    Depositor

    Titia Sixma

    Article

    Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

    Insert

    HOIL-1L (RBCK1 Synthetic, Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synthetic based on human protein sequence

    Promoter

    tac

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCAG-FLAG-puro-PTPIP51(1-150_175-470)

    Plasmid

    #170537

    Purpose

    Expresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfection

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 151-175

    Promoter

    CAG promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

20. pCENPTΔC-dCas9-3xGFP

    Plasmid

    #198325

    Purpose

    Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    CENPTΔC (CENPT Human)
    

    Use

    Lentiviral

    Tags

    EGFP x 3 and dCas9

    Expression

    Mammalian

    Mutation

    truncation - encodes only amino acids 1-375

    Promoter

    CMV-TetO

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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