Addgene

We narrowed to 876 results for: GGCT;

Showing: 441 - 460 of 876 results

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pk335-CARGO-3mer-30kb-DSF

Plasmid

#227486

Purpose

3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 30kb Downstream Fgf5

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-5mer-Fgf5Pro

Plasmid

#227480

Purpose

5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to Fgf5 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-4mer-PrPro

Plasmid

#227455

Purpose

4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to Prdm8 promoter Upstream Prdm8

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-3mer-PrPro

Plasmid

#227456

Purpose

3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to Prdm8 promoter Upstream Prdm8

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP

Plasmid

#234882

Purpose

L1HS-silencing plasmid (CRISPRi gRNA1)

Depositor

Johan Jakobsson

Article

Adami et al bioRxiv 2025.01.17.633315

Insert

L1HS-silencing plasmid (CRISPRi gRNA1)

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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shRNA_RNF170_1

Plasmid

#215206

Purpose

Supression of shRNF170_1 expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

RNF170 shRNA 1 (RNF170 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDL649

Plasmid

#231164

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlHAIRLESS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

mobile gRNA targeting SlHAIRLESS

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDY2259 hNOLC1 atgRNA Paired Guide 1

Plasmid

#220989

Purpose

hNOLC1 atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

NOLC1 atgRNA (NOLC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDY2261 hACTB atgRNA Paired Guide 1

Plasmid

#220991

Purpose

hACTB atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

ACTB atgRNA (ACTB Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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RhoC gRNA #1

Plasmid

#198724

Purpose

Cas9-mediated knockout of RhoC in mammalian cells #1

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoC (Rhoc Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-2O-crEGFP_EF1a-BFP

Plasmid

#224784

Purpose

EGFP targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and non-target BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

crEGFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

hU6-2xTetO

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-CrEGFP_EF1a-BFP

Plasmid

#224786

Purpose

EGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

crBFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

hU6 and hU6-2xTetO

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-SNRPA_sgRNA1

Plasmid

#201622

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SNRPA (SNRPA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgGPAA1 guide 2

Plasmid

#193609

Purpose

GPAA1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgGPAA1 guide 2 (GPAA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSCL.179

Plasmid

#185000

Purpose

Express -Eco1 HEK4 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Use

Tags

SV40NLS

Expression

Mammalian

Mutation

HEK4 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-v2-ALDH1A3gRNA2

Plasmid

#189747

Purpose

The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

Cas9, ALDH1A3 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-PBD-GFP-U6ac-rac2 guides

Plasmid

#168251

Purpose

"label active Rac under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

PBD GFP

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

LyzC

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides

Plasmid

#168254

Purpose

"label stable F-actin under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Utrophin-GFP

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

LyzC

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mU6-sgCebpa_v2-hu6-sgCebpb_v2

Plasmid

#177252

Purpose

Expresses Cebpa_v2 (mU6), Cebpb_v2(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_v2/sgCebpb_v2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

mU6/hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mU6-sgCebpb_v2-hu6-sgCebpd_v2

Plasmid

#177256

Purpose

Expresses Cebpb_v2 (mU6), Cebpd_v2(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpb_v2/sgCebpd_v2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

mU6/hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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