We narrowed to 15,997 results for: LEA

1. mCerulean3-Bik-s2193

   Plasmid

   #177425

   Purpose

   To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bik, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

   Depositor

   David Andrews

   Insert

   Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
   

   Tags

   mCerulean3

   Expression

   Mammalian

   Promoter

   Human ferritin

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRE3GV-somBiPOLES-mCerulean

   Plasmid

   #192578

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of tetTag-positive neurons

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Promoter

   TRE3GV

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PvLEA4_repeats_k3_26_mCh-SspB (pBS1075)

   Plasmid

   #185299

   Purpose

   Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   pvLEA22mer_shuffle_3
   

   Expression

   Mammalian

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pvLEA22mer_shuffle_3_mCh-2xFKBP (pBS1119)

   Plasmid

   #185314

   Purpose

   For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   pvLEA22mer_shuffle_3
   

   Expression

   Mammalian

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. LEA1_APHAV_Chakrabortee pSecTag (pBS0254)

   Plasmid

   #185143

   Purpose

   For the mammalian expression of the nematode (Aphelenchus avenae) protein LEA1_APHAV. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   LEA1_APHAV
   

   Expression

   Mammalian

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pvLEA22mer_mutant_3_mCh-2xFKBP (pBS1123)

   Plasmid

   #185318

   Purpose

   For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   pvLEA22mer_mutant_3
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PvLEA4_repeats_k5_1_mCh-2xFKBP (pBS1122)

   Plasmid

   #185317

   Purpose

   For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   PvLEA4_repeats_k5_1
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pvLEA22mer_mutant_10_mCh-2xFKBP (pBS1121)

   Plasmid

   #185316

   Purpose

   For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   pvLEA22mer_mutant_10
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PvLEA4_repeats_k3_26_mCh-2xFKBP (pBS1118)

   Plasmid

   #185313

   Purpose

   For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   PvLEA4_repeats_k3_26
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. miniCMV-mCerulean-tDeg

    Plasmid

    #185401

    Purpose

    mCerulean-tDeg fluorogenic protein

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

    Insert

    mCerulean-tDeg
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pvLEA22mer_mutant_3_mCh-SspB (pBS1080)

    Plasmid

    #185303

    Purpose

    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_mutant_3
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PvLEA4_repeats_k5_1_mCh-SspB (pBS1079)

    Plasmid

    #185302

    Purpose

    For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k5_1
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pvLEA22mer_mutant_10_mCh-SspB (pBS1078)

    Plasmid

    #185301

    Purpose

    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_mutant_10
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Scarlet I-FRET 10-Cerulean3

    Plasmid

    #139374

    Purpose

    FRET Standard

    Depositor

    Richard Day

    Article

    Day et al PLoS One. 2021 Dec 23;16(12):e0261660. d

    Insert

    Cerulean3 N1
    

    Expression

    Mammalian

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTK-EdnrbE2-Cerulean

    Plasmid

    #127771

    Purpose

    Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Cerulean activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    ednrbE2
    

    Use

    Enhancer reporter construct

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK-T7G10 leader

    Plasmid

    #123548

    Purpose

    MoChlo kit

    Depositor

    Scott Lenaghan, C. Neal Stewart

    Article

    Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

    Insert

    T7G10 leader
    

    Use

    Synthetic Biology

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK-PpsbD-leader

    Plasmid

    #123482

    Purpose

    MoChlo kit

    Depositor

    Scott Lenaghan, C. Neal Stewart

    Article

    Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

    Insert

    PpsbD-leader
    

    Use

    Synthetic Biology

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS2-his-24::Cerulean

    Plasmid

    #124324

    Purpose

    Expressing Nuclear Localized Cerulean

    Depositor

    Andrew Leifer

    Insert

    Cerulean (his-24 Nematode)
    

    Expression

    Worm

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKK-TEV-mCerulean

    Plasmid

    #105790

    Purpose

    Expression of your protein of interest in fusion with cyan fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCerulean

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKK-mCerulean-TEV

    Plasmid

    #105773

    Purpose

    Expression of your protein of interest in fusion with cyan fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mCerulean-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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