We narrowed to 1,707 results for: abat

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pLentiCRISPR sgFTCD_2

Plasmid

#102313

Purpose

genetic depletion of FTCD

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgFTCD_2 (FTCD Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-Kaptin

Plasmid

#100511

Purpose

Expresses FLAG tagged Kaptin in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

KPTN (KPTN Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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HcRed-pcw107

Plasmid

#64646

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Promoter

PGK

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv1_COX4

Plasmid

#177983

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting COX4

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting COX4

Use

Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgCTRL-1

Plasmid

#83934

Purpose

Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCTRL-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgCTRL-2

Plasmid

#83935

Purpose

Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCTRL-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgCTRL-3

Plasmid

#83936

Purpose

Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCTRL-3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgAMDHD1

Plasmid

#102316

Purpose

genetic depletion of AMDHD1

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgAMDHD1 (AMDHD1 Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pK RagA(Q66L)-RagC(Q120L)

Plasmid

#99702

Purpose

Bacterial expression of RagA(Q66L)-RagC(Q120L)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

Q120L and Q66L

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLJC5-Tmem192-3xHA

Plasmid

#102930

Purpose

Lentiviral expression of a lysosomal tag (Tmem192-HA)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

Tmem192 (TMEM192 Human)

Use

Lentiviral

Tags

HA

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLJC6-3XHA-TMEM192

Plasmid

#104434

Purpose

Expresses 3XHA-TMEM192 in mammalian cells

Depositor

David Sabatini

Article

Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

3XHA

Expression

Mammalian

Promoter

Ubiquitin

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLJC5-Tmem192-2xFlag

Plasmid

#102929

Purpose

Lentiviral expression of a lysosomal tag (Tmem192-Flag)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

Tmem192 (TMEM192 Human)

Use

Lentiviral

Tags

Flag

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-E1799K

Plasmid

#69009

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-E1799K (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Glutamate 1799 to Lysine

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-F1888L

Plasmid

#69010

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-F1888L (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Phenylalanine 1888 to Leucine

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only
pLJM60 FLAG-RAB35 Q67L

Plasmid

#107105

Purpose

Lentiviral vector that codes for FLAG-RAB35 Q67L (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

Depositor

Charles Sawyers

Article

Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

Insert

RAB35 (RAB35 Human)

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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Flag WDR24 pRK5

Plasmid

#46334

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR24 (WDR24 Human)

Tags

FLag

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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LAMP1-mRFP-FLAG

Plasmid

#34611

Purpose

3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag

Depositor

David Sabatini

Article

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

2xFlag and mRFP

Expression

Mammalian

Available Since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FNIP2

Plasmid

#72294

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP2 (FNIP2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
DPYD-FLAG

Plasmid

#61616

Purpose

The human DPYD was used to study the effect of DPYD expression on EMT

Depositor

David Sabatini

Article

Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

Insert

Dihydropyrimidine dehydrogenase (DPYD Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Cterminal HA WDR59 pRK5

Plasmid

#46328

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR59 (WDR59 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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