We narrowed to 6,971 results for: crispr cas9 plasmids

1. NAX_CAGGS_CAS9_VPR_KanR neo

   Plasmid

   #167893

   Purpose

   PiggyBac compatible plasmid expressing Cas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

   Plasmid

   #184294

   Purpose

   A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

   Depositor

   Bernardo Sabatini

   Insert

   sgRNA-Adrb1
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Grp

   Plasmid

   #184292

   Purpose

   A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Grp

   Depositor

   Bernardo Sabatini

   Insert

   sgRNA-Grp
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Oprl1

   Plasmid

   #184291

   Purpose

   A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Oprl1

   Depositor

   Bernardo Sabatini

   Insert

   sgRNA-Oprl1
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Rxfp3

   Plasmid

   #184290

   Purpose

   A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Rxfp3

   Depositor

   Bernardo Sabatini

   Insert

   sgRNA-Rxfp3
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

   Plasmid

   #184295

   Purpose

   A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

   Depositor

   Bernardo Sabatini

   Insert

   sgRNA-Adrb1
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti-Cas9-gRNA-GFP

   Plasmid

   #124770

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-Cas9-gRNA-TagBFP2

   Plasmid

   #124774

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EC2_2_dCas9_VP64_sgRNA

   Plasmid

   #163707

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VP64+SV40 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-SMVP-Cas9N

    Plasmid

    #80930

    Purpose

    Expresses Cas9N in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

    Plasmid

    #74010

    Purpose

    Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

    Depositor

    Filippo Del Bene

    Article

    Di Donato et al Genome Res. 2016 Mar 8.

    Inserts

    5xUAS
    Cas9
    Cre
    U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9-EcRT-Z3-Hyg

    Plasmid

    #232095

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    Z3 promoter and Z3EV transcription factor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Z3

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. SETD2(SET)-Cas9

    Plasmid

    #186700

    Purpose

    Plasmid encoding SETD2-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    SETD2(SET)-Cas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCXLE-dCas9GFP-shP53

    Plasmid

    #102906

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9GFP-shP53
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCas9-EcRT-Z3-Nat

    Plasmid

    #232094

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    Z3 promoter and Z3EV transcription factor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Z3

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCas9-EcRT-Z3-Kan

    Plasmid

    #232096

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    Z3 promoter and Z3EV transcription factor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Z3

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Native promoter dCas9

    Plasmid

    #113656

    Purpose

    dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    Native promoter and dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    S. pyogenes Cas9 native promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adra1b

    Plasmid

    #184293

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adra1b

    Depositor

    Bernardo Sabatini

    Inserts

    sgRNA-Adra1b
    sgRNA-Adra1b
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

    Plasmid

    #221385

    Purpose

    Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene

    Depositor

    Junwei Shi

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-RSV-SpCas9

    Plasmid

    #85450

    Purpose

    Express SpCas9 in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pRSV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pRSV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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