We narrowed to 14,084 results for: cas9

1. CD55-Delta3

   Plasmid

   #220872

   Purpose

   Expresses CD55 variant in mammalian cells

   Depositor

   Ferenc Scheeren

   Insert

   CD55 Delta-3
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Delta-3, short consensus repeat (SCR) domains 1, …

   Promoter

   CMV

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CD55-Delta4

   Plasmid

   #220873

   Purpose

   Expresses CD55 variant in mammalian cells

   Depositor

   Ferenc Scheeren

   Insert

   CD55 Delta-4
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Delta-4, short consensus repeat (SCR) domains 1, …

   Promoter

   CMV

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CD55-Delta1

   Plasmid

   #220870

   Purpose

   Expresses CD55 variant in mammalian cells

   Depositor

   Ferenc Scheeren

   Insert

   CD55 Delta-1
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Delta-1, the first short consensus repeat (SCR) d…

   Promoter

   CMV

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CD55-Delta2

   Plasmid

   #220871

   Purpose

   Expresses CD55 variant in mammalian cells

   Depositor

   Ferenc Scheeren

   Insert

   CD55 Delta-2
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Delta-2, short consensus repeat (SCR) domains 1 a…

   Promoter

   CMV

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPN10

   Plasmid

   #114386

   Purpose

   Empty gRNA

   Depositor

   Vincent Dion

   Article

   Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKozak_mTagBFP2-T2A-sGFP11x7_synCoTC

   Plasmid

   #199495

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   mTagBFP2-T2A-sGFP11x7
   

   Use

   CRISPR

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSA_0_mTagBFP2-T2A-sGFP11x7_synCoTC

   Plasmid

   #199478

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   mTagBFP2-T2A-sGFP11x7
   

   Use

   CRISPR

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSA_1_mTagBFP2-T2A-sGFP11x7_synCoTC

   Plasmid

   #199479

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   mTagBFP2-T2A-sGFP11x7
   

   Use

   CRISPR

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSA_2_mTagBFP2-T2A-sGFP11x7_synCoTC

   Plasmid

   #199480

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   mTagBFP2-T2A-sGFP11x7
   

   Use

   CRISPR

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSD_2xNES-FRB-ZFPTS2-3xFLAG-2xNLS

    Plasmid

    #107303

    Purpose

    Expresses ZFP-VEGFA-TS2 fused to FRB in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    ZFP_VEGFA-TS2
    

    Use

    CRISPR

    Tags

    2xNES, 3x Flag, 2xNLS, and FRB

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSD_2xNES-FRB-ZFPTS3-3xFLAG-2xNLS

    Plasmid

    #107304

    Purpose

    Expresses ZFP-VEGFA-TS3 fused to FRB in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    ZFP_VEGFA-TS3
    

    Use

    CRISPR

    Tags

    2xNES, 3x Flag, 2xNLS, and FRB

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHSN6I01

    Plasmid

    #50587

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-KRAB
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Mutation

    dCas9 that is defective in DNA cleavage; the maiz…

    Promoter

    2×35Sp and AtU6-26p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMEL13

    Plasmid

    #107919

    Purpose

    kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMEL12

    Plasmid

    #107918

    Purpose

    hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pROS10

    Plasmid

    #107924

    Purpose

    URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHSN6A01

    Plasmid

    #50586

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    dCas9-VP64
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG, HA, and NLS

    Mutation

    dCas9 = defective in DNA cleavage; 4×minimal VP16…

    Promoter

    2×35Sp and AtU6-26p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS31-cCARLIN

    Plasmid

    #160928

    Purpose

    Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)

    Depositor

    Fernando Camargo

    Article

    Bowling et al Cell. 2020 Jun 11;181(6):1410-1422.e27.

    Insert

    CARLIN gRNAs and CAG-GFP-CARLIN array
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6 promoter for gRNAs; CAG promoter for GFP

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PEA1-Puro

    Plasmid

    #171991

    Purpose

    Delivers all prime editing nickase components in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Expression

    Mammalian

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPtGE34

    Plasmid

    #107932

    Purpose

    Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

    Depositor

    David Edgell

    Article

    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

    Inserts

    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    

    Use

    CRISPR and Synthetic Biology; Episomal vector for…

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PEA1-GFP

    Plasmid

    #171993

    Purpose

    Delivers all prime editing nickase components in a single, GFP selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Expression

    Mammalian

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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