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We narrowed to 12,110 results for: NSI

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Showing: 9241 - 9280 of 12110 results

  3. Split 'n coexpress CUL3-RBX1

    Plasmid
    #52293
    Purpose
    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1
    Depositor
    Brenda Schulman
    Article
    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1
    Inserts
    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    Tags
    GST, IRES, and TEV
    Expression
    Bacterial
    Mutation
    contains aa 1-309, contains aa 310-768, and conta…
    Promoter
    IRES and tac
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

    Plasmid
    #177335
    Purpose
    bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177335-plasmid-map-sequence-id-348601

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  5. pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE

    Plasmid
    #172908
    Purpose
    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence
    Depositor
    GENIE Project
    Article
    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-
    Has Service
    AAV1
    Insert
    Ace2N-4AA-mNeon-ST A122D
    Use
    AAV and Cre/Lox
    Tags
    soma localization targeting sequence
    Expression
    Mammalian
    Mutation
    A122D
    Promoter
    hSynapsin1
    Available Since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172908-plasmid-map-sequence-id-341477

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  7. MIP 227 CoMiP 4in1 wo shRNA p53

    Plasmid
    #63727
    Purpose
    To express Oct4, Klf4, Sox2, and c-Myc. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).
    Depositor
    Mark Kay, Joseph Wu
    Article
    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103
    Insert
    Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC Human)
    Tags
    IRES-dtomato
    Expression
    Mammalian
    Mutation
    codon-optimised
    Promoter
    SFFV
    Available Since
    Aug. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    63727-plasmid-map-sequence-id-114454

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  8. MIP 247 CoMiP 4in1 with shRNA p53

    Plasmid
    #63726
    Purpose
    To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).
    Depositor
    Mark Kay, Joseph Wu
    Article
    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103
    Insert
    Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC, TP53 Human)
    Tags
    IRES-dtomato
    Expression
    Mammalian
    Mutation
    codon-optimised
    Promoter
    SFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53
    Available Since
    Aug. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    63726-plasmid-map-sequence-id-438433

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  11. Ma-PylRS-lib1

    Pooled Library
    #224585
    Purpose
    Pyrrolysine tRNA synthetase library to generate new genetic code expansion (GCE) systems for site-specific incorporation of non-canonical amino acids (ncAAs) into proteins.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Expression
    Bacterial
    Species
    Methanomethylophilus alvus
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only

  12. pTUBmCardinalSagCAT.dna

    Plasmid
    #166236
    Purpose
    mCardinal consituitive reporter, CAT marker
    Depositor
    David Sibley
    Insert
    mCardinal
    Mutation
    none
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166236-plasmid-map-sequence-id-329836

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  13. pLEW100cre_del_tetO

    Plasmid
    #24019
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Trypanosome transient expression
    Tags
    Cre Recombinase
    Available Since
    May 11, 2010
    Availability
    Academic Institutions and Nonprofits only
    24019-plasmid-map-sequence-id-11290

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  14. pcDNA3.1-ccdB-V5

    Plasmid
    #214985
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal V5 tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Tags
    V5
    Expression
    Mammalian
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214985-plasmid-map-sequence-id-440020

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  15. pJC226

    Plasmid
    #198637
    Purpose
    MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system
    Depositor
    Shaun Curtin
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    June 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198637-plasmid-map-sequence-id-394770

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  16. pTJK459

    Plasmid
    #138008
    Purpose
    Empty sgRNA expression vector for expression of sgRNA for Sp Cas9
    Depositor
    Tami Kingsbury
    Type
    Empty backbone
    Use
    Lentiviral
    Mutation
    U-A flip and hairpin extension: cgtttAagagctaTGCT…
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138008-plasmid-map-sequence-id-275002

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  17. pGLU_103

    Plasmid
    #225785
    Purpose
    This is the promoter region taken from upstream of Tn5 transposase in pBAM1. It can be considered the "default" promoter to use.
    Depositor
    Nili Ostrov
    Insert
    Ptn5_[Tn5]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225785-plasmid-map-sequence-id-446997

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  18. pcDNA3.1-ccdB-3xFLAG

    Plasmid
    #214984
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xFLAG tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Tags
    3xFLAG
    Expression
    Mammalian
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214984-plasmid-map-sequence-id-440018

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  19. pcDNA3.1-ccdB-3xHA

    Plasmid
    #214986
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xHA tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Tags
    3xHA
    Expression
    Mammalian
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214986-plasmid-map-sequence-id-440021

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  20. pcDNA3.1-ccdB-TwinStrep

    Plasmid
    #214988
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal TwinStrep tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Tags
    TwinStrep
    Expression
    Mammalian
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214988-plasmid-map-sequence-id-440022

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  21. pGLU_85

    Plasmid
    #225800
    Purpose
    This is the promoter region taken from upstream of Himar transposase in pMarC9-Kan. It can be considered the "default" promoter to use.
    Depositor
    Nili Ostrov
    Insert
    PmarC9_[Himar]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    225800-plasmid-map-sequence-id-446917

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  26. pLY1244-T4gp44/62

    Plasmid
    #228205
    Purpose
    Expression in BL21(DE3) of WT T4 clamp loader consisting of gp44 and gp62
    Depositor
    Stephen Benkovic
    Insert
    44/62
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    228205-plasmid-map-sequence-id-472133

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  27. pT7-NSP5SClow

    Plasmid
    #220829
    Purpose
    Express the Rotavirus NSP5 protein with a potentially lower propensity to form viroplasms
    Depositor
    Alex Borodavka
    Insert
    Rotavirus NSP5SClow
    Use
    Other
    Available Since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    220829-plasmid-map-sequence-id-443938

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  28. pET15b (I-WcaINhis) K226I

    Plasmid
    #195620
    Purpose
    Expresses I-WcaI homing endonuclease from Wickerhamomyces canadensis containing the K226I mutation
    Depositor
    Frederick Gimble
    Insert
    I-WcaI
    Expression
    Bacterial
    Mutation
    A1930T, A1931T
    Promoter
    T7
    Available Since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    195620-plasmid-map-sequence-id-384568

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  29. pET15b (I-WcaINhis) K125I

    Plasmid
    #195619
    Purpose
    Expresses I-WcaI homing endonuclease from Wickerhamomyces canadensis containing the K125I mutation
    Depositor
    Frederick Gimble
    Insert
    I-WcaI
    Expression
    Bacterial
    Mutation
    A2030T, A2031T
    Promoter
    T7
    Available Since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    195619-plasmid-map-sequence-id-384567

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Showing: 9241 - 9280 of 12110 results